Project description
Understanding plant transcriptome regulations during environmental changes
Plants constantly adapt to changing environments, with climate change intensifying these stresses. RNA modifications, play a key role in regulating RNA behaviour, aiding plant adaptation and survival. Understanding how non-genetic factors influence the plant transcriptome during heat stress (HS) is crucial for modern agriculture. Supported by the Marie Skłodowska-Curie Actions programme, the MemoryRNA project aims to identify transcriptome-wide m5C and m6A modifications that contribute to cellular responses to HS, characterise these marks on thermomemory mRNAs, and investigate the regulation and function of the m6A reader protein ECT2 in transcriptional regulation at the shoot apical meristem. The project will leverage advanced technologies to validate and characterise m5C- and m6A-containing mRNAs.
Objective
Plants live in constantly changing environments and the increased frequency of extreme weather, as a result of climate change, will exacerbate such stresses. The MemoryRNA project aims to expand the fundamental knowledge of non-genetically-based regulations of the plant transcriptome during heat stress (HS). Post-transcriptional modifications of RNA create an additional layer to reversibly control RNA fate. The sophisticated and poorly understood thermomemory phenomenon represents a powerful system to study the role of the RNA modifications and its high impact on RNA metabolism to reprogram the transcriptome, thus leading plants to adapt and survive. To unravel the role of epitranscriptomic-based reprogramming of the transcriptome of thermomemory RNAs at the shoot apical meristem, the project aims to: i) Reveal the m5C and m6A modifications transcriptome-wide that actively contribute to the global cellular response to HS; ii) Characterize the m5C and m6A marks on thermomemomery mRNAs; and iii) Determine how a m6A reader protein ECT2 is regulated upon HS, and its functional role on transcriptional regulation. Data and biological resources obtained by the team support the MemoryRNA project and incorporate a comprehensive set of cutting-edge technologies and approaches, including: i) Direct sequencing of long-read fragments and computational analysis for accurate mapping of RNA marks; ii) Antibody-based techniques and mutant plants to validate and characterise the function of m5C- and m6A-containing mRNAs; and iii) RNA and fluorescence in situ hybridization, and laser confocal microscopy to investigate the role of ECT2 during thermomemory. Understanding the mechanisms that coordinate transcriptional activity of HS-memory genes in response to environmental stressors is of utmost importance to modern agriculture, and throughout the the funding period, opportunities for translation of our results to biotechnological application will be monitored and explored.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy
- agricultural sciences agriculture, forestry, and fisheries agriculture
- natural sciences biological sciences genetics RNA
- natural sciences physical sciences optics laser physics
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
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Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2022-PF-01
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
14469 Potsdam
Germany
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