MIcrobe-synthesised DNA NAnostructures for DIsplay-controlled Storage Cartridges

Información del proyecto

MI-DNA DISC

Identificador del acuerdo de subvención: 101115215

DOI

10.3030/101115215

Fecha de la firma de la CE 21 Junio 2023

Fecha de inicio 1 Septiembre 2023

Fecha de finalización 31 Agosto 2027

Financiado con arreglo a

• The European Innovation Council (EIC)

Coste total

€ 3 999 506,50

Aportación de la UE

€ 3 999 506,50

Coordinado por

AIT AUSTRIAN INSTITUTE OF TECHNOLOGY GMBH
Austria

Periodic Reporting for period 2 - MI-DNA DISC (MIcrobe-synthesised DNA NAnostructures for DIsplay-controlled Storage Cartridges)

Período documentado: 2024-09-01 hasta 2026-02-28

Resumen del contexto y de los objetivos generales del proyecto

The ongoing digitalisation is leading to a massive increase of data generated. Conventional storing technologies can only be upscaled to a certain degree due to increasing material and energy costs. DNA is a promising storing medium due to its high data density and stability. Our vision is to develop a low-cost, energy-efficient, and fast data drive that is able to write, edit, store, and retrieve DNA-based data. The data drive is based on simple and easily available hardware components plus bacterial cells. To achieve this vision, we will exploit bacterial genetic mechanisms that were evolutionarily optimised for billions of years, such as colour-sensitive genetic switches and DNA exchange processes. We have defined two specific objectives to achieve our goal.
The first objective is to create a pipeline that converts digital data into DNA nanostructures with increased biostability compared to conventional duplex DNA.
The second objective is to develop a storing cartridge that uses bacteria for the storage of the DNA-based information.

Trabajo realizado desde el comienzo del proyecto hasta el final del período abarcado por el informe y los principales resultados hasta la fecha

In the first 2,5 years of the project, we developed a coding and encoding scheme. To evaluate these process, we further developed a digital twin that simulates the DNA writing and sequencing processes considering writing and sequencing errors as well as mutations in bacteria. We published a publicly available webserver: DNAbyte.ekorefugium.com. In addition, we developed in silico tools to reduce wetlab experiments.
Further, we developed technologies to control the uptake of DNA in bacteria via light-based mechanisms.

Avances que van más allá del estado de la técnica e impacto potencial esperado (incluida la repercusión socioeconómica y las implicaciones sociales más amplias del proyecto hasta la fecha)

The encoding and writing of data using an error robust oligonucleotide library and the development of optogenetic tools for DNA uptake represent results beyond the state of the art.