MIcrobe-synthesised DNA NAnostructures for DIsplay-controlled Storage Cartridges

Informations projet

MI-DNA DISC

N° de convention de subvention: 101115215

DOI

10.3030/101115215

Date de signature de la CE 21 Juin 2023

Date de début 1 Septembre 2023

Date de fin 31 Août 2027

Financé au titre de

• The European Innovation Council (EIC)

Coût total

€ 3 999 506,50

Contribution de l’UE

€ 3 999 506,50

Coordonné par

AIT AUSTRIAN INSTITUTE OF TECHNOLOGY GMBH
Austria

Periodic Reporting for period 1 - MI-DNA DISC (MIcrobe-synthesised DNA NAnostructures for DIsplay-controlled Storage Cartridges)

Période du rapport: 2023-09-01 au 2024-08-31

Résumé du contexte et des objectifs généraux du projet

The ongoing digitalisation is leading to a massive increase of data generated. Conventional storing technologies can only be upscaled to a certain degree due to increasing material and energy costs. DNA is a promising storing medium due to its high data density and stability. Our vision is to develop a low-cost, energy-efficient, and fast data drive that is able to write, edit, store, and retrieve DNA-based data. The data drive is based on simple and easily available hardware components plus bacterial cells. To achieve this vision, we will exploit bacterial genetic mechanisms that were evolutionarily optimised for billions of years, such as colour-sensitive genetic switches and DNA exchange processes. We have defined two specific objectives to achieve our goal.
The first objective is to create a pipeline that converts digital data into DNA nanostructures with increased biostability compared to conventional duplex DNA.
The second objective is to develop a storing cartridge that uses bacteria for the storage of the DNA-based information.

Travail effectué depuis le début du projet jusqu’à la fin de la période considérée dans le rapport et principaux résultats atteints jusqu’à présent

In the first year of the project, we started to develop a coding and encoding scheme. To evaluate these process, we have developed a digital twin that simulates the DNA writing and sequencing processes considering writing and sequencing errors as well as mutations in bacteria. In addition, we developed in silico tools to reduce wetlab experiments.
In a second work package, we started to evaluate bacteria that are most suitable for DNA data storage. We have selected three species that are suited for the development of the proof-of-concept as well as long-term storage. Further, we analysed genetic tools to manipulate bacterial DNA processes.

Progrès au-delà de l’état des connaissances et impact potentiel prévu (y compris l’impact socio-économique et les conséquences sociétales plus larges du projet jusqu’à présent)

Since we started from a very low TRL (1), no results significantly above the state of the art were achieved in the first year.