MIcrobe-synthesised DNA NAnostructures for DIsplay-controlled Storage Cartridges

Projektinformationen

MI-DNA DISC

ID Finanzhilfevereinbarung: 101115215

DOI

10.3030/101115215

EK-Unterschriftsdatum 21 Juni 2023

Startdatum 1 September 2023

Enddatum 31 August 2027

Finanziert unter

• The European Innovation Council (EIC)

Gesamtkosten

€ 3 999 506,50

EU-Beitrag

€ 3 999 506,50

Koordiniert durch

AIT AUSTRIAN INSTITUTE OF TECHNOLOGY GMBH
Austria

Periodic Reporting for period 2 - MI-DNA DISC (MIcrobe-synthesised DNA NAnostructures for DIsplay-controlled Storage Cartridges)

Berichtszeitraum: 2024-09-01 bis 2026-02-28

Zusammenfassung vom Kontext und den Gesamtzielen des Projekts

The ongoing digitalisation is leading to a massive increase of data generated. Conventional storing technologies can only be upscaled to a certain degree due to increasing material and energy costs. DNA is a promising storing medium due to its high data density and stability. Our vision is to develop a low-cost, energy-efficient, and fast data drive that is able to write, edit, store, and retrieve DNA-based data. The data drive is based on simple and easily available hardware components plus bacterial cells. To achieve this vision, we will exploit bacterial genetic mechanisms that were evolutionarily optimised for billions of years, such as colour-sensitive genetic switches and DNA exchange processes. We have defined two specific objectives to achieve our goal.
The first objective is to create a pipeline that converts digital data into DNA nanostructures with increased biostability compared to conventional duplex DNA.
The second objective is to develop a storing cartridge that uses bacteria for the storage of the DNA-based information.

Arbeit, die ab Beginn des Projekts bis zum Ende des durch den Bericht erfassten Berichtszeitraums geleistet wurde, und die wichtigsten bis dahin erzielten Ergebnisse

In the first 2,5 years of the project, we developed a coding and encoding scheme. To evaluate these process, we further developed a digital twin that simulates the DNA writing and sequencing processes considering writing and sequencing errors as well as mutations in bacteria. We published a publicly available webserver: DNAbyte.ekorefugium.com. In addition, we developed in silico tools to reduce wetlab experiments.
Further, we developed technologies to control the uptake of DNA in bacteria via light-based mechanisms.

Fortschritte, die über den aktuellen Stand der Technik hinausgehen und voraussichtliche potenzielle Auswirkungen (einschließlich der bis dato erzielten sozioökonomischen Auswirkungen und weiter gefassten gesellschaftlichen Auswirkungen des Projekts)

The encoding and writing of data using an error robust oligonucleotide library and the development of optogenetic tools for DNA uptake represent results beyond the state of the art.