MIcrobe-synthesised DNA NAnostructures for DIsplay-controlled Storage Cartridges

Informazioni relative al progetto

MI-DNA DISC

ID dell’accordo di sovvenzione: 101115215

DOI

10.3030/101115215

Data della firma CE 21 Giugno 2023

Data di avvio 1 Settembre 2023

Data di completamento 31 Agosto 2027

Finanziato da

• The European Innovation Council (EIC)

Costo totale

€ 3 999 506,50

Contributo UE

€ 3 999 506,50

Coordinato da

AIT AUSTRIAN INSTITUTE OF TECHNOLOGY GMBH
Austria

Periodic Reporting for period 2 - MI-DNA DISC (MIcrobe-synthesised DNA NAnostructures for DIsplay-controlled Storage Cartridges)

Periodo di rendicontazione: 2024-09-01 al 2026-02-28

Sintesi del contesto e degli obiettivi generali del progetto

The ongoing digitalisation is leading to a massive increase of data generated. Conventional storing technologies can only be upscaled to a certain degree due to increasing material and energy costs. DNA is a promising storing medium due to its high data density and stability. Our vision is to develop a low-cost, energy-efficient, and fast data drive that is able to write, edit, store, and retrieve DNA-based data. The data drive is based on simple and easily available hardware components plus bacterial cells. To achieve this vision, we will exploit bacterial genetic mechanisms that were evolutionarily optimised for billions of years, such as colour-sensitive genetic switches and DNA exchange processes. We have defined two specific objectives to achieve our goal.
The first objective is to create a pipeline that converts digital data into DNA nanostructures with increased biostability compared to conventional duplex DNA.
The second objective is to develop a storing cartridge that uses bacteria for the storage of the DNA-based information.

Lavoro eseguito dall’inizio del progetto fino alla fine del periodo coperto dalla relazione e principali risultati finora ottenuti

In the first 2,5 years of the project, we developed a coding and encoding scheme. To evaluate these process, we further developed a digital twin that simulates the DNA writing and sequencing processes considering writing and sequencing errors as well as mutations in bacteria. We published a publicly available webserver: DNAbyte.ekorefugium.com. In addition, we developed in silico tools to reduce wetlab experiments.
Further, we developed technologies to control the uptake of DNA in bacteria via light-based mechanisms.

Progressi oltre lo stato dell’arte e potenziale impatto previsto (incluso l’impatto socioeconomico e le implicazioni sociali più ampie del progetto fino ad ora)

The encoding and writing of data using an error robust oligonucleotide library and the development of optogenetic tools for DNA uptake represent results beyond the state of the art.