Collaborative development and dissemination of workflows and techniques for using Correlative Light, Electron and X-ray Microscopy to progress research into the understanding and treatment of diseases

Información del proyecto

CLEXM

Identificador del acuerdo de subvención: 101120151

DOI

10.3030/101120151

Fecha de la firma de la CE 9 Junio 2023

Fecha de inicio 1 Septiembre 2023

Fecha de finalización 31 Agosto 2027

Financiado con arreglo a

• Marie Skłodowska-Curie Actions (MSCA)

Coste total

Sin datos

Aportación de la UE

€ 2 480 594,40

Coordinado por

UNIVERSITY COLLEGE DUBLIN, NATIONAL UNIVERSITY OF IRELAND, DUBLIN
Ireland

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Other (2)

Conference Success

Report on the conferences delivered throughout the project, including copies of papers presented as well as metrics of attendees. Note that this Deliverable will be reopened for update at M48

Workshop Success

Interim Report on the workshops delivered throughout the project, including copies of training materials as well as metrics of attendees. Note that this Deliverable will be reopened for update at M48

Documents, reports (2)

Cell-prep workflow

Develop a cryo-cell preparation workflow that will allow cells to be imaged across a combination of FM/EM/SXT instruments

Micropatterned grids

Report describing the results of testing cryo-cell preparation on micropatterned grids.

Websites, patent fillings, videos etc. (1)

Website

Project Website

Publicaciones

Conference proceedings (4)

Integrating Cryo Soft X-Ray Tomography into CLEM and Volume EM Correlative Workflows

Autores: Sergey Kapishnikov, Paul Sheridan, William Fyans, Fergal O’Reilly, Tony McEnroe, Kenneth Fahy
Publicado en: Microscopy and Microanalysis, Edición 31, 2025, ISSN 1431-9276
Editor: Oxford University Press (OUP)
DOI: 10.1093/MAM/OZAF048.503

Spatially-Informed Multi-Stage Active Learning for Segmentation of Nucleus in Soft X-ray Tomography

Autores: Ayse Erozan, Alexander Zeilmann, Anthoula Chatzimpinou, Fariha Mahzabin Annesha, Vincent Heuveline, Venera Weinhardt
Publicado en: 2025 IEEE Symposium on Computational Intelligence in Image, Signal Processing and Synthetic Media (CISM), 2025
Editor: IEEE
DOI: 10.1109/CISM64958.2025.11060865

Elimination of HCV replication machinery early after antiviral treatment with DAA monitored by multimodal microscopy

Autores: Victoria Castro, Gema Calvo, Ana Pérez-Berna, David Rogers, Stephen O'Connor, Sergey Kapishnikov, Paul Sheridan, Eva Pereiro, Kenneth Fahy, Pablo Gastaminza
Publicado en: BIO Web of Conferences, Edición 129, 2024, ISSN 2117-4458
Editor: EDP Sciences
DOI: 10.1051/BIOCONF/202412918008

SARS-CoV-2 and HCV infection and antiviral treatment monitored by multimodal imaging

Autores: Ana Joaquina Perez-Berna, Kevin Mamprin, Victoria Castro, Gema Calvo, Ricardo Valcárcel, Eva Pereiro, Pablo Gastaminza
Publicado en: BIO Web of Conferences, Edición 129, 2024, ISSN 2117-4458
Editor: EDP Sciences
DOI: 10.1051/BIOCONF/202412916002

Peer reviewed articles (11)

Harnessing 3D cell models and high-resolution imaging to unveil the mechanisms of nanoparticle-mediated drug delivery

Autores: Alannah S. Chalkley, Maëva T. Lopez, Margaritha M. Mysior, Madeleen C. Brink, Suainibhe Kelly, Jeremy C. Simpson
Publicado en: Frontiers in Bioengineering and Biotechnology, Edición 13, 2025, ISSN 2296-4185
Editor: Frontiers Media SA
DOI: 10.3389/FBIOE.2025.1606573

The bacterial type three secretion system induces mechanoporation of vacuolar membranes

Autores: Léa Swistak, Marvin Albert, Camila Valenzuela, Elif Begum Gokerkucuk, François Bontems, Stéphane Tachon, Keith T. Egger, Anastasia D. Gazi, Anna Sartori-Rupp, Cammie F. Lesser, Perrine Paul-Gilloteaux, Jean-Yves Tinevez, Matthijn Vos, Jost Enninga
Publicado en: PLOS Biology, Edición 23, 2025, ISSN 1545-7885
Editor: Public Library of Science (PLoS)
DOI: 10.1371/JOURNAL.PBIO.3003135

<scp>CELS</scp>‐<scp>3D</scp>—Cutting edge light source for exciting fluorescence in microtome‐based <scp>3D</scp> microscopy and targeted correlative microscopy

Autores: Vladyslav Denyshchenko, Christopher Evans, Tiina O'Neill, Jakub Krstev, Emilia Filipczak, Silas O'Toole, Vladimir Lobaskin, Dimitri Scholz
Publicado en: Journal of Anatomy, Edición 246, 2024, ISSN 0021-8782
Editor: Wiley
DOI: 10.1111/JOA.14164

Histidine-modified UiO-66(Zr) nanoparticles as an effective pH-responsive carrier for 5-fluorouracil drug delivery system: A possible pathway to more effective brain cancer treatments

Autores: Alexandra Migasová, Ľuboš Zauška, Tomáš Zelenka, Dominik Volavka, Marta Férová, Terézia Gulyásová, Silvia Tomková, Michaela Saláková, Veronika Kuchárová, Tomáš Samuely, Jozef Bednarčík, Cyril Slabý, Mariana Máčajová, Boris Bilčík, Tim Schubert, Andreas Walter, Virginie Hornebecq, Veronika Huntošová, Miroslav Almáši
Publicado en: Chemical Engineering Journal, Edición 522, 2025, ISSN 1385-8947
Editor: Elsevier BV
DOI: 10.1016/J.CEJ.2025.167857

Rapid HCV Replication Machinery Removal after Antiviral Treatment with DAA Monitored by Multimodal Imaging

Autores: Victoria Castro, Gema Calvo, Ana Pérez-Berna, David Rogers, Stephen O’Connor, Sergey Kapishnikov, Paul Sheridan, Eva Pereiro, Kenneth Fahy, Pablo Gastaminza
Publicado en: Microscopy and Microanalysis, Edición 30, 2024, ISSN 1431-9276
Editor: Oxford University Press (OUP)
DOI: 10.1093/MAM/OZAE044.1056

Cryogenic Super-Resolution Fluorescence, Electron, and X-ray Microscopy -- A Novel Correlative Nanoscale Imaging Workflow to Reveal Nanoparticle Internalization into Cancer Cells

Autores: Pavitra Sokke Rudraiah, Louisa Herbsleb, Michaela Salakova, Henriette Gröger, Anna Maria Steyer, Frauke Alves, Claus Feldmann, Andreas Walter
Publicado en: 2025, ISSN 0362-4331
Editor: Wiley
DOI: 10.22541/AU.174798562.25512020/V1

Rapid hepatitis C virus replication machinery removal after antiviral treatment with DAA monitored by multimodal imaging

Autores: Victoria Castro, Gema Calvo, Ana J. Pérez-Berná, Kevin Mamprin, Sergey Kapishnikov, David Rogers, Stephen O'Connor, Paul Sheridan, Kenneth Fahy, Eva Pereiro, Pablo Gastaminza
Publicado en: Structure, Edición 33, 2025, ISSN 0969-2126
Editor: Elsevier BV
DOI: 10.1016/J.STR.2025.05.015

The VapBC Toxin–Antitoxin System Enhances<i>Shigella flexneri</i>Fitness Through Coordination of Metabolic Stress Adaptation and Virulence

Autores: Estelle Saifi, Elisabeth Ageron, Keith Egger, Caroline Reisacher, Eric Frapy, Morgan Lamberioux, Shelley Payne, Jost Enninga, Laurence Arbibe
Publicado en: 2025, ISSN 2692-8205
Editor: Cold Spring Harbor Laboratory
DOI: 10.1101/2025.06.29.662172

Super-resolution mapping of the ankle link proteins ADGRV1 and PDZD7 in developing auditory hair cells

Autores: Baptiste Colcombet-Cazenave, Gaël Moneron, Antonio El Helou, David DiGregorio, Vincent Michel, Nicolas Wolff
Publicado en: iScience, Edición 28, 2025, ISSN 2589-0042
Editor: Elsevier BV
DOI: 10.1016/J.ISCI.2025.113190

Soft X-Ray Reveals Chromatin Remodeling in Human CD4 T Cells

Autores: Fariha Mahzabin Annesha, Ayse Erozan, Swetha Ananth, Oliver T Fackler, Venera Weinhardt
Publicado en: Microscopy and Microanalysis, Edición 31, 2025, ISSN 1431-9276
Editor: Oxford University Press (OUP)
DOI: 10.1093/MAM/OZAF048.393

Soft X-ray tomography reveals variations in B. subtilis biofilm structure upon tasA deletion

Autores: Chatzimpinou, Anthoula; Diehl, Anne; Harhoff, A Tobias; Driller, Kristina; Vanslembrouck, Bieke; Chen, Jian-Hua; Kairišs, Kristaps; Loconte, Valentina; Le Gros, Mark A; Larabell, Carolyn; Turgay, Kürşad; Oschkinat, Hartmut; Weinhardt, Venera
Publicado en: npj Biofilms and Microbiomes, 2025, ISSN 2055-5008
Editor: npj Biofilms Microbiomes
DOI: 10.1038/S41522-025-00659-0