Collaborative development and dissemination of workflows and techniques for using Correlative Light, Electron and X-ray Microscopy to progress research into the understanding and treatment of diseases

Projektinformationen

CLEXM

ID Finanzhilfevereinbarung: 101120151

DOI

10.3030/101120151

EK-Unterschriftsdatum 9 Juni 2023

Startdatum 1 September 2023

Enddatum 31 August 2027

Finanziert unter

• Marie Skłodowska-Curie Actions (MSCA)

Gesamtkosten

Keine Daten

EU-Beitrag

€ 2 480 594,40

Koordiniert durch

UNIVERSITY COLLEGE DUBLIN, NATIONAL UNIVERSITY OF IRELAND, DUBLIN
Ireland

CORDIS bietet Links zu öffentlichen Ergebnissen und Veröffentlichungen von HORIZONT-Projekten.

Links zu Ergebnissen und Veröffentlichungen von RP7-Projekten sowie Links zu einigen Typen spezifischer Ergebnisse wie Datensätzen und Software werden dynamisch von OpenAIRE abgerufen.

Leistungen

Other (2)

Conference Success

Report on the conferences delivered throughout the project, including copies of papers presented as well as metrics of attendees. Note that this Deliverable will be reopened for update at M48

Workshop Success

Interim Report on the workshops delivered throughout the project, including copies of training materials as well as metrics of attendees. Note that this Deliverable will be reopened for update at M48

Documents, reports (2)

Cell-prep workflow

Develop a cryo-cell preparation workflow that will allow cells to be imaged across a combination of FM/EM/SXT instruments

Micropatterned grids

Report describing the results of testing cryo-cell preparation on micropatterned grids.

Websites, patent fillings, videos etc. (1)

Website

Project Website

Veröffentlichungen

Conference proceedings (4)

Integrating Cryo Soft X-Ray Tomography into CLEM and Volume EM Correlative Workflows

Autoren: Sergey Kapishnikov, Paul Sheridan, William Fyans, Fergal O’Reilly, Tony McEnroe, Kenneth Fahy
Veröffentlicht in: Microscopy and Microanalysis, Ausgabe 31, 2025, ISSN 1431-9276
Herausgeber: Oxford University Press (OUP)
DOI: 10.1093/MAM/OZAF048.503

Spatially-Informed Multi-Stage Active Learning for Segmentation of Nucleus in Soft X-ray Tomography

Autoren: Ayse Erozan, Alexander Zeilmann, Anthoula Chatzimpinou, Fariha Mahzabin Annesha, Vincent Heuveline, Venera Weinhardt
Veröffentlicht in: 2025 IEEE Symposium on Computational Intelligence in Image, Signal Processing and Synthetic Media (CISM), 2025
Herausgeber: IEEE
DOI: 10.1109/CISM64958.2025.11060865

Elimination of HCV replication machinery early after antiviral treatment with DAA monitored by multimodal microscopy

Autoren: Victoria Castro, Gema Calvo, Ana Pérez-Berna, David Rogers, Stephen O'Connor, Sergey Kapishnikov, Paul Sheridan, Eva Pereiro, Kenneth Fahy, Pablo Gastaminza
Veröffentlicht in: BIO Web of Conferences, Ausgabe 129, 2024, ISSN 2117-4458
Herausgeber: EDP Sciences
DOI: 10.1051/BIOCONF/202412918008

SARS-CoV-2 and HCV infection and antiviral treatment monitored by multimodal imaging

Autoren: Ana Joaquina Perez-Berna, Kevin Mamprin, Victoria Castro, Gema Calvo, Ricardo Valcárcel, Eva Pereiro, Pablo Gastaminza
Veröffentlicht in: BIO Web of Conferences, Ausgabe 129, 2024, ISSN 2117-4458
Herausgeber: EDP Sciences
DOI: 10.1051/BIOCONF/202412916002

Peer reviewed articles (11)

Harnessing 3D cell models and high-resolution imaging to unveil the mechanisms of nanoparticle-mediated drug delivery

Autoren: Alannah S. Chalkley, Maëva T. Lopez, Margaritha M. Mysior, Madeleen C. Brink, Suainibhe Kelly, Jeremy C. Simpson
Veröffentlicht in: Frontiers in Bioengineering and Biotechnology, Ausgabe 13, 2025, ISSN 2296-4185
Herausgeber: Frontiers Media SA
DOI: 10.3389/FBIOE.2025.1606573

The bacterial type three secretion system induces mechanoporation of vacuolar membranes

Autoren: Léa Swistak, Marvin Albert, Camila Valenzuela, Elif Begum Gokerkucuk, François Bontems, Stéphane Tachon, Keith T. Egger, Anastasia D. Gazi, Anna Sartori-Rupp, Cammie F. Lesser, Perrine Paul-Gilloteaux, Jean-Yves Tinevez, Matthijn Vos, Jost Enninga
Veröffentlicht in: PLOS Biology, Ausgabe 23, 2025, ISSN 1545-7885
Herausgeber: Public Library of Science (PLoS)
DOI: 10.1371/JOURNAL.PBIO.3003135

<scp>CELS</scp>‐<scp>3D</scp>—Cutting edge light source for exciting fluorescence in microtome‐based <scp>3D</scp> microscopy and targeted correlative microscopy

Autoren: Vladyslav Denyshchenko, Christopher Evans, Tiina O'Neill, Jakub Krstev, Emilia Filipczak, Silas O'Toole, Vladimir Lobaskin, Dimitri Scholz
Veröffentlicht in: Journal of Anatomy, Ausgabe 246, 2024, ISSN 0021-8782
Herausgeber: Wiley
DOI: 10.1111/JOA.14164

Histidine-modified UiO-66(Zr) nanoparticles as an effective pH-responsive carrier for 5-fluorouracil drug delivery system: A possible pathway to more effective brain cancer treatments

Autoren: Alexandra Migasová, Ľuboš Zauška, Tomáš Zelenka, Dominik Volavka, Marta Férová, Terézia Gulyásová, Silvia Tomková, Michaela Saláková, Veronika Kuchárová, Tomáš Samuely, Jozef Bednarčík, Cyril Slabý, Mariana Máčajová, Boris Bilčík, Tim Schubert, Andreas Walter, Virginie Hornebecq, Veronika Huntošová, Miroslav Almáši
Veröffentlicht in: Chemical Engineering Journal, Ausgabe 522, 2025, ISSN 1385-8947
Herausgeber: Elsevier BV
DOI: 10.1016/J.CEJ.2025.167857

Rapid HCV Replication Machinery Removal after Antiviral Treatment with DAA Monitored by Multimodal Imaging

Autoren: Victoria Castro, Gema Calvo, Ana Pérez-Berna, David Rogers, Stephen O’Connor, Sergey Kapishnikov, Paul Sheridan, Eva Pereiro, Kenneth Fahy, Pablo Gastaminza
Veröffentlicht in: Microscopy and Microanalysis, Ausgabe 30, 2024, ISSN 1431-9276
Herausgeber: Oxford University Press (OUP)
DOI: 10.1093/MAM/OZAE044.1056

Cryogenic Super-Resolution Fluorescence, Electron, and X-ray Microscopy -- A Novel Correlative Nanoscale Imaging Workflow to Reveal Nanoparticle Internalization into Cancer Cells

Autoren: Pavitra Sokke Rudraiah, Louisa Herbsleb, Michaela Salakova, Henriette Gröger, Anna Maria Steyer, Frauke Alves, Claus Feldmann, Andreas Walter
Veröffentlicht in: 2025, ISSN 0362-4331
Herausgeber: Wiley
DOI: 10.22541/AU.174798562.25512020/V1

Rapid hepatitis C virus replication machinery removal after antiviral treatment with DAA monitored by multimodal imaging

Autoren: Victoria Castro, Gema Calvo, Ana J. Pérez-Berná, Kevin Mamprin, Sergey Kapishnikov, David Rogers, Stephen O'Connor, Paul Sheridan, Kenneth Fahy, Eva Pereiro, Pablo Gastaminza
Veröffentlicht in: Structure, Ausgabe 33, 2025, ISSN 0969-2126
Herausgeber: Elsevier BV
DOI: 10.1016/J.STR.2025.05.015

The VapBC Toxin–Antitoxin System Enhances<i>Shigella flexneri</i>Fitness Through Coordination of Metabolic Stress Adaptation and Virulence

Autoren: Estelle Saifi, Elisabeth Ageron, Keith Egger, Caroline Reisacher, Eric Frapy, Morgan Lamberioux, Shelley Payne, Jost Enninga, Laurence Arbibe
Veröffentlicht in: 2025, ISSN 2692-8205
Herausgeber: Cold Spring Harbor Laboratory
DOI: 10.1101/2025.06.29.662172

Super-resolution mapping of the ankle link proteins ADGRV1 and PDZD7 in developing auditory hair cells

Autoren: Baptiste Colcombet-Cazenave, Gaël Moneron, Antonio El Helou, David DiGregorio, Vincent Michel, Nicolas Wolff
Veröffentlicht in: iScience, Ausgabe 28, 2025, ISSN 2589-0042
Herausgeber: Elsevier BV
DOI: 10.1016/J.ISCI.2025.113190

Soft X-Ray Reveals Chromatin Remodeling in Human CD4 T Cells

Autoren: Fariha Mahzabin Annesha, Ayse Erozan, Swetha Ananth, Oliver T Fackler, Venera Weinhardt
Veröffentlicht in: Microscopy and Microanalysis, Ausgabe 31, 2025, ISSN 1431-9276
Herausgeber: Oxford University Press (OUP)
DOI: 10.1093/MAM/OZAF048.393

Soft X-ray tomography reveals variations in B. subtilis biofilm structure upon tasA deletion

Autoren: Chatzimpinou, Anthoula; Diehl, Anne; Harhoff, A Tobias; Driller, Kristina; Vanslembrouck, Bieke; Chen, Jian-Hua; Kairišs, Kristaps; Loconte, Valentina; Le Gros, Mark A; Larabell, Carolyn; Turgay, Kürşad; Oschkinat, Hartmut; Weinhardt, Venera
Veröffentlicht in: npj Biofilms and Microbiomes, 2025, ISSN 2055-5008
Herausgeber: npj Biofilms Microbiomes
DOI: 10.1038/S41522-025-00659-0