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Unlocking a T cell-mediated Immune response in therapy-challenged Tumors

Project description

Tumour microenvironment insights for durable therapy

The tumour microenvironment (TME) refers to the complex and dynamic environment surrounding a tumour, and which includes various cell types, extracellular matrix components and signalling molecules. The TME plays a critical role in tumour progression, immune responses and treatment efficacy. The ERC-funded UnlockIT project aims to investigate the complex biology of the TME under therapeutic challenge, hoping to unveil mechanisms of drug resistance and immune evasion. Moreover, the study will focus on antigen processing and presentation in the TME aiming to make cancer cells more visible to the immune system. The idea behind UnlockIT is to provide the fundamental knowledge needed for developing combination anticancer therapies with durable effect.

Objective

Immuno- and targeted therapies have revolutionized the treatment of cancer patients, and more innovative therapeutics, such as KRAS inhibitors (KRASi), are entering the clinic. However, their use as monotherapies fails to achieve durable tumor control in most patients, and the development of combination therapies remains challenging without an understanding of the complex and dynamic biology that unfolds upon therapeutic challenge in the tumor microenvironment (TME). Most of our knowledge on combination therapies is based on primary, treatment-naïve tumors, but how signaling networks of cancer cells and their interactions with immune cells evolve under therapeutic challenge over time is poorly understood, and strategies to overcome immune-evasive TMEs are lacking. Here, we build on our expertise in modeling the dynamic changes of cancer cells in all phases of therapy to move towardsstrategic, mechanism-based combination therapies.
Our vision is to Unlock a T cell-mediated Immune response in therapy-challenged Tumors, “UnlockIT”, to achieve durable therapy responses in cancer patients. (1) We will use our improved molecular time machine, CaTCHseq, together with novel KRASi and unique KRAS-driven tumor models to understand tumor-TME co-evolution under KRASi and related mechanisms of drug-resistance. (2) We will characterize mediators of antigen processing and presentation in treatment naïve, therapy-challenged and immune evasive cancer cell states by using state-of-the-art genome-wide CRISPR/Cas9 screens in combination with innovative phenotypic readouts. This approach offers a sought-after opportunity to uncover mechanisms that make cancer cells “visible” to immunity. (3) Finally, we aim to overcome immune-evasive TMEs by understanding and targeting antigen-presenting cells in the TME. Taken together, UnlockIT will provide new mechanistic links between oncogenic pathways and immunity, and thereby establish a rational basis for combining targeted and immunotherapies.

Fields of science (EuroSciVoc)

CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.

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Host institution

FORSCHUNGSINSTITUT FUR MOLEKULARE PATHOLOGIE GESELLSCHAFT MBH
Net EU contribution
€ 2 000 000,00
Address
CAMPUS-VIENNA-BIOCENTER 1
1030 Wien
Austria

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Region
Ostösterreich Wien Wien
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Links
Total cost
€ 2 000 000,00

Beneficiaries (1)

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