Description du projet
Un changement durable dans la conservation des cellules
Le bilan environnemental des méthodes conventionnelles de mise en banque de données biologiques est indéniable. La congélation dans l’azote liquide (LN) a un coût élevé, tant sur le plan économique qu’écologique, car elle nécessite des installations spécialisées et contribue à une empreinte CO2 considérable. Soutenu par le programme Actions Marie Skłodowska-Curie, le projet WhyNotDry vise à révolutionner les biobanques en examinant le séchage réversible des cellules et du germoplasme, dans le but de remplacer la congélation LN par une alternative innovante, rentable et respectueuse de l’environnement. Ce projet pluridisciplinaire implique un réseau international de scientifiques qui s’engagent à libérer le potentiel des biobanques sèches pour un avenir durable dans le domaine des sciences de la vie. Le projet ne se contente pas de remodeler les méthodes de conservation, il favorise également la collaboration interdisciplinaire et promet un changement de paradigme «vert» au sein de la communauté scientifique.
Objectif
WhyNotDry will deepen our knowledge on reversible drying in cells and germplasm toward the development of a dry biobanking as an alternative to the current freezing in Liquid Nitrogen (LN). LN is expensive, requires dedicated facilities and power supply, and has a high CO2 footprint. WhyNotDry will achieve its aims through a multidisciplinary, intersectoral, international network of scientists that will: i) Develop a drying/rehydration platform using naturally desiccation-tolerant midge (Polypedilum vanderplanki) Pv11 cell lines; ii) Identify the best combination of naturally occurring xeroprotectants (xero=dry) from desiccation-tolerant insect cell line and the best performing water subtraction platform. iii) To use the best xeroprotectants mix and the drying/rehydration protocol for mammalian cells/germplasm. vi) Develop a prototype for controlled dehydration of microvolumes of cell. The R&I activities leading to these aims will be carried out by knowhow sharing through Staff Exchanges between: 3 EU academies, 2 EU SMEs, 2 international partners (Japan, Thailand). Outcomes of WhyNotDry will be incorporated into a cheap, environmentally friendly, and easy biobanking for biodiversity conservation, assisted reproduction, stem cell/personalized medicine. Successful development of this technology will set the basis for a radically new, ‘green’ biobanking paradigm, simplifying the maintenance and shipping practices in life sciences, with enormous reduction in costs and carbon footprint. Moreover, knowledge generated in WhyNotDry would be applicable to other fields such as agriculture, environmental science, food processing, and the pharmaceutical industry, where elective or enforced (by climate change) drying is dealt with. Finally, WhyNotDry will empower young scientists with transferable skills, ensuring career prospects in academia/industry, and strengthen the international/sectorial network between disciplines, boosting European excellence.
Champ scientifique
- natural sciencesearth and related environmental sciencesenvironmental sciences
- medical and health sciencesmedical biotechnologycells technologiesstem cells
- natural sciencesbiological sciencesbiodiversity conservation
- medical and health scienceshealth sciencespersonalized medicine
- natural sciencesearth and related environmental sciencesatmospheric sciencesclimatologyclimatic changes
Programme(s)
- HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA) Main Programme
Régime de financement
HORIZON-TMA-MSCA-SE - HORIZON TMA MSCA Staff ExchangesCoordinateur
64100 Teramo
Italie