Projektbeschreibung
Die Rolle von Lipidtröpfchen bei der Infektion mit Pflanzenviren erhellen
Pflanzenzellen speichern Lipide in spezialisierten Strukturen, den sogenannten Lipidtröpfchen, die als Energiequelle und Bausteine für die Membranbiogenese und andere zelluläre Prozesse, einschließlich des Zellstoffwechsels, dienen. Ziel des über die Marie-Skłodowska-Curie-Maßnahmen finanzierten Projekts RAPT ist es, die Rolle von Lipidtröpfchen bei der Replikation von Pflanzenviren wie dem Rübenmosaikvirus und dem Gerstengelbverzwergungsvirus zu untersuchen. Ausgehend von der Beobachtung, dass virusinfizierte Blätter mehr Lipidtröpfchen enthalten, werden die Forschenden untersuchen, ob letztere als Energiequellen für die Virusinfektion dienen. Im Rahmen des Projekts wird erforscht, wie Viren mit Lipidtröpfchen interagieren, mit dem Ziel, Strategien zur Verbesserung der Pflanzenproduktivität zu entwickeln.
Ziel
The RAPT (lipid dRoplets And PhyTovirus infection) project undertakes an innovative exploration into the potential role of plant leaf lipid droplets (LDs) in the replication of plant viruses, specifically focusing on plant RNA viruses. Lipid droplets, which are ubiquitous organelles derived from endoplasmic reticulum (ER) membranes, play key roles in energy production, membrane biogenesis, and stress signalling. While certain animal RNA viruses utilize LDs for energy or as platforms for viral replication compartments (VRCs), evidence for similar mechanisms in plant RNA viruses remains elusive. The project builds on the previous observations made by the host lab, which suggest a higher abundance of LDs in turnip mosaic virus (TuMV, potyvirus) infected leaves and their spatial proximity to VRCs. The hypothesis is that certain plant viruses, like TuMV, utilize LDs as energy reservoirs for their genome replication, potentially facilitating direct interaction with VRCs. To validate and expand upon this hypothesis, the RAPT project is defined into three primary objectives:
1. Exploring the impact of plant virus infection on LD distribution and lipid metabolism
2. Functional validation of the involvement of plant LDs in the potyvirus infection cycle
3. Elucidating the molecular mechanisms governing the interactions between LDs and TuMV
These objectives address key knowledge gaps regarding the role of LDs in plant virus infection and will focus on potyviruses, mainly TuMV, but other (+) ssRNA virus species of agroeconomic importance such as luteoviruses and poleroviruses will also be studied, with the aim to expand the findings to crops. The results of this project will contribute to the development of broad-spectrum genetic resistances that can be harnessed by breeders to mitigate the adverse impacts of plant viral diseases on agricultural productivity.
Wissenschaftliches Gebiet
- agricultural sciencesagriculture, forestry, and fisheriesagricultureagronomyplant protection
- natural sciencesbiological sciencescell biologycell signaling
- natural sciencesbiological sciencesmicrobiologyvirology
- natural sciencesbiological sciencesbiochemistrybiomoleculeslipids
- social scienceseconomics and businesseconomicsproduction economicsproductivity
Schlüsselbegriffe
Programm/Programme
- HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA) Main Programme
Aufforderung zur Vorschlagseinreichung
Andere Projekte für diesen Aufruf anzeigenFinanzierungsplan
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European FellowshipsKoordinator
75007 Paris
Frankreich