3D bioprinting construct for the diminishing of bone tumor and progression of bone tissue regeneration: Diagnosis of phenotyping in the cellular model and screening as a therapeutic platform

Descripción del proyecto

Hidrogel bioimpreso en tres dimensiones para el tratamiento de tumores óseos

La ingeniería de tejidos óseos ofrece una solución prometedora para restaurar el tejido óseo dañado. Sin embargo, los métodos actuales se enfrentan a limitaciones a la hora de crear modelos de enfermedad para identificar genes diana en la recidiva tumoral, lo que dificulta la creación de estrategias preclínicas. En este contexto, el equipo del proyecto TissueEng, financiado con fondos europeos, pretende utilizar la bioimpresión tridimensional (3D) para crear un constructo 3D impregnado de biosensores que permita detectar el comportamiento de los tumores óseos y la recuperación de defectos óseos. El objetivo es crear un hidrogel multifuncional bioimpreso en 3D que imite modelos de la enfermedad e identifique dianas para el tratamiento del osteosarcoma. En el proyecto se adoptará un método multidisciplinario. Se espera que los resultados repercutan en la sostenibilidad de la sanidad pública y beneficien a pacientes de todos los niveles socioeconómicos.

Objetivo

Bone tissue engineering has been considered a viable solution Bone tissue engineering has been considered a viable solution for clinical restoration and recovery of defected bone tissue caused by injuries, skeletal dysfunction, and cancer. But current approaches are limited in developing cellular disease models to screen the target genes in tumour relapsing, which enables finding suitable preclinical strategies (animal model). The project will use 3D bioprinting to create an external field driving biosensor-impregnated 3D construct as a potential therapeutic platform for screening bone tumour behaviours and multidisciplinary approaches to bone defect recovery. Hence, the main objective is to determine if the formulation of chiro-magnetic nano-assembly incorporated electro-conductive hydrogel will be able to mimic the in vivo disease model to identify the target of expression in the osteosarcoma model, which will enable the finding of an effective therapeutic strategy for osteosarcoma treatment with gender diversity. The project will focus on the multidisciplinary approach (material science, biochemistry, and cell biology) in the formulation of the multifunctional 3D bioprinting hydrogel (visible light based photo-cross-linkable). The 3D construct will have tunable physical properties such as mechanical stability, chiro-magnetic, etc in determining all stages of cellular behaviour; magnetothermal activity in killing tumour cells; and electro-stimulation mediated osteointegration in tissue regeneration. The project will include the transfer of the candidate’s knowledge to the host and acquiring extensive training from UAveiro on advanced tools and techniques as a part of the career development plan. The results will have a potential impact on humankind and patients from all socio-economical levels, and they are in line with the EU mission to have a significant economic impact on the sustainability of public healthcare care for low-to medium-earning patients' families.