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Paving the way for off-the-shelf adoptive Treg therapy using epigenetic forward programming

Project description

Universal Treg therapy for immune disorders

Autoimmune diseases and organ transplant rejection often cause harmful inflammation, which regulatory T cells (Tregs) can help control. Tregs hold promise as a ‘living drug’ for precision medicine yet customising them for each patient presents major logistical and economic hurdles. The ERC-funded epi-hiPSC-Treg project offers an innovative alternative: developing universal off-the-shelf Treg products using human induced pluripotent stem cells (hiPSCs). This approach aims to differentiate hiPSCs into functional Tregs using epigenetic editing, modifying their DNA structure and gene expression. Building on prior research from the ERC EpiTune project, the team aims to pioneer a clinically viable Treg therapy. If successful, it could lead to scalable therapeutic solutions for treating autoimmune conditions and transplant complications.

Objective

Regulatory T cells (Treg) are a type of immune cell that can suppress harmful inflammation caused by autoimmune diseases or organ transplant rejection. Using Treg as a living drug is a promising form of precision medicine, but it faces many practical and economic challenges, such as the need to produce a specific cell product for each patient. A more feasible solution would be to create off-the-shelf Treg products that are readily available and can be used universally without customizing them for each patient. One way to do this is to use human induced pluripotent stem cells (hiPSCs), which can be differentiated into many different cell types. However, for Tregs, this has not been achieved yet in a clinically relevant way.
In this proof of concept study, we will test our novel method of epigenetic editing on hiPSCs, which can modify the DNA structure and gene expression of the cells, to make them differentiate into functional Treg for their application in therapy. This method is based on our previous work in the ERC starting grant project EpiTune. If successful, it will open new avenues for the production of off-the-shelf therapeutic Treg products, which would mean a very important innovation for the field of Treg therapy.
For this project, we assembled a team of experts who have experience in developing and testing new T cell therapy products in the clinic, including regulatory affairs experts, IP protection professionals, and marketing strategists. If the PoC succeeds, their advice and guidance will help us prepare for the next steps of developing a clinical-grade manufacturing process.

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HORIZON-ERC-POC - HORIZON ERC Proof of Concept Grants

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Call for proposal

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(opens in new window) ERC-2024-POC

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Host institution

CHARITE - UNIVERSITAETSMEDIZIN BERLIN
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 150 000,00
Address
Chariteplatz 1
10117 Berlin
Germany

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Region
Berlin Berlin Berlin
Activity type
Higher or Secondary Education Establishments
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Total cost

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Beneficiaries (1)

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