Project description
Visualising live bacterial infection pathways
Bacterial pathogens like Yersinia pseudotuberculosis must sense and rapidly adapt to host conditions to evade immune responses, replicate and establish infection. Understanding how these dynamic host-pathogen interactions operate is central for the design of precision therapies and for tackling antibiotic resistance. With the support of the Marie Skłodowska-Curie Actions programme, the InfPathMapper project is working on a super-resolution microscopy (SRM) platform for visualising individual infection events in real time. SRM goes beyond the resolution limits of standard light microscopy and enables imaging at the nanoscale of molecular events inside living cells. Researchers will combine SRM with machine learning to study infection pathways and host responses in an attempt to obtain valuable insight into infection at an early stage.
Objective
Pathogens like Yersinia pseudotuberculosis must rapidly adapt to environmental cues to progress through infection, manipulating host responses to survive and replicate. Understanding these adaptive host-pathogen interactions is key to developing targeted therapies, combating antibiotic resistance, and preventing chronic infections. However, current methods miss rare, transient infection stages, limiting our understanding of infection pathways. My project aims to address this by developing InfPathMapper, a data-driven super-resolution microscopy (SRM) platform to map infection pathways in living cells at molecular resolution.
While advances in fluorescence microscopy provide detailed molecular insights, they often struggle with phototoxicity, limited spatiotemporal resolution, and reliance on fixed samples, missing dynamic information of infection processes. My approach will extend a data-driven microscopy platform by integrating adaptive SRM modalities for live-cell imaging of Y. pseudotuberculosis infection, a pathogen with virulence mechanisms conserved across many bacteria. This system will be able to map pathways of individual infection events at super-resolution level in a live-cell fluorescence-based infection assay supported by machine learning to classify pathogen invasion stages and host cell response patterns. Live-to-fix super-resolution mapping of the molecular architecture of critical infection stages will provide new insights into how Yersinia adapts to niche-specific environments and host-induced stress. By combining cutting-edge microscopy with infection biology, the project will not only set me on an excellent pathway toward achieving research independence, it is also expected to contribute valuable knowledge and tools for the broader research community, fostering innovation in the study of other pathogens and dynamic intracellular processes and the fight against infectious diseases.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences physical sciences optics microscopy super resolution microscopy
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2024-PF-01
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
22100 Lund
Sweden
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.