Obiettivo We are building a parallel and independent (orthogonal) translational machinery for the encoded biosynthesis of unnatural polymers in living cells. The orthogonal translation system has many potential applications beyond those possible with the natural translation system: I propose to use it: 1) To expand the chemical scope of monomers that can be polymerized by the ribosome in living cells, allowing the incorporation of monomers with unnatural backbones into proteins; 2) To increase the efficiency of in vivo unnatural amino acid mutagenesis via amber suppression, so that no truncated protein is produced and multi-site incorporation of unnatural amino acids is possible; 3) To create probes of protein function for use in vivo; 4) To free numerous codons for simultaneous encoding of multiple distinct unnatural monomers, and to experimentally explore alternate genetic codes; 5) To explore the evolution of encoded unnatural polymers toward new cellular functions. Campo scientifico natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural scienceschemical sciencespolymer sciencesnatural scienceschemical sciencesorganic chemistryamines Parole chiave genetic genetic code expansion ribosome engineering synthetic biology Programma(i) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Argomento(i) ERC-SG-LS7 - Applied life sciences, biotechnology and bioengineering: agricultural, animal, fishery, forestry/food sciences; biotechnology, chemical biology, genetic engineering, synthetic biology, industrial biosciences; environmental biotechnology. Invito a presentare proposte ERC-2007-StG Vedi altri progetti per questo bando Meccanismo di finanziamento ERC-SG - ERC Starting Grant Istituzione ospitante MEDICAL RESEARCH COUNCIL Contributo UE € 1 782 918,00 Indirizzo 20 Park Crescent W1B 1AL LONDON Regno Unito Mostra sulla mappa Tipo di attività Public bodies (excluding Research Organisations and Secondary or Higher Education Establishments) Ricercatore principale Jason William Karl Chin (Dr.) Contatto amministrativo Elizabeth Cutler (Ms.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato Beneficiari (1) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto MEDICAL RESEARCH COUNCIL Regno Unito Contributo UE € 1 782 918,00 Indirizzo 20 Park Crescent W1B 1AL LONDON Mostra sulla mappa Tipo di attività Public bodies (excluding Research Organisations and Secondary or Higher Education Establishments) Ricercatore principale Jason William Karl Chin (Dr.) Contatto amministrativo Elizabeth Cutler (Ms.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato
