Obiettivo Cellular protection against oxidative damage is relevant to ageing and numerous complex diseases. We plan to use fission yeast as a model organism to gain a systems-level understanding of the oxidative stress response and obtain insights into the interplay of variable genotype, phenotype, and environment. To this end, the four participants will pursue a range of multi-disciplinary and complementing approaches that will be integrated with innovative computational methods for a unified view of a complete regulatory system. We will create a genetically and phenotypically diverse library of yeast strains from crosses of three independent parental strains with distinct stress sensitivities. The parental and segregant strains will be genotyped and phenotyped (in stressed and unstressed cells) using state-of-the-art sequencing, tiling array, and proteomics approaches, thus providing a rich basis for genome-wide association studies and computational modelling. Genetic, functional genomic, and proteomic approaches, along with computational methods, will be applied in parallel to develop protein and gene interaction networks that will further support the modelling efforts. Predictions based on the modelling will be validated with targeted wet-lab experiments to test and refine the mathematical models. Intimate inter-dependency between experimental and bioinformatic approaches based on close collaboration among participants with different expertise will be vital to develop successful models predicting the regulatory response to oxidative stress. The relative simplicity of yeast cells, which can be grown under tightly controlled conditions and with defined genetic and environmental perturbations, promises a thorough and deep understanding of the oxidative stress response system. Concepts developed in the proposed study will provide a valuable framework for research into more complex systems such as response networks and association studies in human cells. Campo scientifico scienze naturaliscienze biologichebiochimicabiomolecoleproteineproteomicascienze naturaliinformatica e scienze dell'informazionescienze computazionaliscienze naturalimatematicamatematica applicatamodello matematico Parole chiave oxidative stress pathway modelling posttranscriptional regulation quantitative trait loci Programma(i) FP7-HEALTH - Specific Programme "Cooperation": Health Argomento(i) HEALTH-2007-2.1.2-5 - Multidisciplinary fundamental genomics and molecular biology approaches to study basic biological processes relevant to health and diseases HEALTH-2007-3.1-4 - Improving clinical decision making Invito a presentare proposte FP7-HEALTH-2007-B Vedi altri progetti per questo bando Meccanismo di finanziamento CP-FP - Small or medium-scale focused research project Coordinatore TECHNISCHE UNIVERSITAET DRESDEN Indirizzo Helmholtzstrasse 10 01069 Dresden Germania Mostra sulla mappa Regione Sachsen Dresden Dresden, Kreisfreie Stadt Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo Sven Kreigenfeld (Mr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Contributo UE Nessun dato Partecipanti (3) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto DANMARKS TEKNISKE UNIVERSITET Danimarca Contributo UE € 544 960,00 Indirizzo Anker engelunds vej 101 2800 Kongens lyngby Mostra sulla mappa Regione Danmark Hovedstaden Københavns omegn Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo Ernst Løwe Lindberg (Mr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Altri finanziamenti Nessun dato EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH Svizzera Contributo UE € 339 800,00 Indirizzo Raemistrasse 101 8092 Zuerich Mostra sulla mappa Regione Schweiz/Suisse/Svizzera Zürich Zürich Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo Rudolf Aebersold (Prof.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Altri finanziamenti Nessun dato UCL Elizabeth Garrett Anderson Institute for Women’s Health Regno Unito Contributo UE € 950 600,00 Indirizzo Gower street WC1E 6BT London Mostra sulla mappa Regione London Inner London — West Camden and City of London Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo Kent Lee (Mr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Altri finanziamenti Nessun dato
