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Molecular Networks of Dopaminergic Neurons in Chordates

Ziel

Background. Parkinson Disease is the second most common progressive neurodegenerative disorder. The selective degeneration of subsets of midbrain dopaminergic neurons is believed to be the primary cause for disruption of the ability to control movements. Objective. We propose to apply a highly interdisciplinary approach to construct complex networks consisting of protein coding genes, non-protein-coding genes and cis-regulatory elements within dopaminergic neurons in the brain across three chordate organisms (Mouse, Zebrafish and Ciona) to identify and compare gene regulatory networks in these neurons. This will be achieved by: • Expression profiling of genes on single dopaminergic neurons via laser microdissection and transgenic lines in Mouse, Ciona and Zebrafish • HT-Sequencing of microCAGE assays on dopaminergic neurons, providing TSS usage and transcript discovery • Microscopy HTS of cis-regulatory elements • siRNA and morpholino network perturbation experiments • Innovative systems biology approaches to decipher and define molecular networks Data generated by microCAGE and microarray will define a set of key genes in dopaminergic neurons, in which cis-regulatory elements will be predicted and screened utilizing HTS in zebrafish. The data will aid network reconstruction, which will be validated by perturbation experiments. This project relies also on the availability of data produced through the many existing collaborations among consortium partners such as FP6 funded TRANSCODE project as well as the international Fantom3 consortium. Potential impact: The prevalence of PD in Europe today is ~2 million people. Within the next 50 years, the number is expected to rise to 5 million. Thus, the burden placed by dementia on the working-age population will rise dramatically. No treatments are known to slow the progression of the disease. Deciphering the basic networks of dopaminergic neurons will generate novel diagnostic and therapeutic candidates.

Wissenschaftliches Gebiet

  • /Medizin- und Gesundheitswissenschaften/Grundlagenmedizin/Neurologie/Parkinson
  • /Naturwissenschaften/Naturwissenschaften/Optik/Laserphysik

Aufforderung zur Vorschlagseinreichung

FP7-HEALTH-2007-B
Andere Projekte für diesen Aufruf anzeigen

Finanzierungsplan

CP-FP - Small or medium-scale focused research project

Koordinator

SCUOLA INTERNAZIONALE SUPERIORE DI STUDI AVANZATI DI TRIESTE
Adresse
Via Bonomea 265
34136 Trieste
Italien
Aktivitätstyp
Higher or Secondary Education Establishments
EU-Beitrag
€ 406 400
Kontakt Verwaltung
Luca Bardi (Mr.)

Beteiligte (9)

CONSORZIO PER IL CENTRO DI BIOMEDICINA MOLECOLARE SCARL
Italien
EU-Beitrag
€ 357 200
Adresse
Strada Statale 14 Km 163 5 Basovizza
34149 Trieste
Aktivitätstyp
Research Organisations
Kontakt Verwaltung
Nicola Dorigo Salamon (Mr.)
RIKEN THE INSTITUTE OF PHYSICAL ANDCHEMICAL RESEARCH
Japan
EU-Beitrag
€ 277 400
Adresse
Hirosawa 2-1
351 0198 Wako Shi Saitama
Aktivitätstyp
Research Organisations
Kontakt Verwaltung
Haruo Yamaguchi (Mr.)
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
Frankreich
EU-Beitrag
€ 279 400
Adresse
Rue Michel Ange 3
75794 Paris
Aktivitätstyp
Research Organisations
Kontakt Verwaltung
Beatrice Saint-Cricq (Ms.)
ALBERT-LUDWIGS-UNIVERSITAET FREIBURG
Deutschland
EU-Beitrag
€ 297 400
Adresse
Fahnenbergplatz
79098 Freiburg
Aktivitätstyp
Higher or Secondary Education Establishments
Kontakt Verwaltung
Wolfgang Driever (Prof.)
THE UNIVERSITY OF BIRMINGHAM
United Kingdom
EU-Beitrag
€ 398 400
Adresse
Edgbaston
B15 2TT Birmingham
Aktivitätstyp
Higher or Secondary Education Establishments
Kontakt Verwaltung
Robert Fekete (Mr.)
KARLSRUHER INSTITUT FUER TECHNOLOGIE
Deutschland
EU-Beitrag
€ 565 980
Adresse
Kaiserstrasse 12
76131 Karlsruhe
Aktivitätstyp
Higher or Secondary Education Establishments
Kontakt Verwaltung
Natascha Wallburg (Ms.)
CLINICAL GENE NETWORKS AB

Beteiligung beendet

Schweden
EU-Beitrag
€ 69 290,40
Adresse
Karolinska Sience Park - Fogdevreten 2
171 77 Stockholm
Aktivitätstyp
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Kontakt Verwaltung
Jesper Tegner (Prof.)
University College London
United Kingdom
EU-Beitrag
€ 230 400
Adresse
Gower Street
WC1E 6BT London
Aktivitätstyp
Higher or Secondary Education Establishments
Kontakt Verwaltung
Greta Borg-Carbott (Ms.)
YH YOUHEALTH AB
Schweden
EU-Beitrag
€ 85 309,60
Adresse
Fogdevreten 2 Karolinska Science
171 77 Stockholm
Aktivitätstyp
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Kontakt Verwaltung
Jesper Tegnér (Prof.)