Objective
Bordetella pertussis, B. parapertussis and B. bronchiseptica are Gram-negative bacteria that colonize the respiratory tracts of mammals. B. pertussis has adapted exclusively to the human host and is known to be the causative agent of whooping cough (pertussis). Bordetella bacteria use the type III secretion system (T3SS) to establish persistent colonization of the trachea and to modulate the host immune response. They encode a highly conserved type III secretion effector protein, BteA, which is responsible for the induction of rapid, non-apoptotic death in a wide range of mammalian cells. BteA, the only type III effector that has been identified in Bordetella, shows no homology with any other type III effectors. The overall goal is to determine BteA structure and to elucidate its involvement in mechanisms leading to cell death. Our specific aims are:
1. To express, purify and determine the 3D structure of BteA from B. bronchiseptica.
2. To analyze BteA structure and determine its functional sites and to determine the ability of BteA to bind ligands.
3. To locate BteA interacting proteins in the eukaryotic host cell by using BteA as bait and thus determine i) protein function; ii) the structure of BteA-target molecule complexes.
Cutting-edge technologies will be used for structure determination and functional analysis of clean protein samples (X-ray crystallography, structure prediction and functional point mutations) and for the investigation of ligand binding and host cell interactions (pull-down assays, mass spectrometry and surface plasmon resonance).
The outcome will be new targets for antibiotic development and a better understanding of protein-protein interactions, cell targeting and cell death. The research will enhance EU competitiveness in structural biology and the life sciences and in the long term will contribute to public health (treatment and prevention of whooping cough).
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences health sciences public health
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- medical and health sciences basic medicine immunology
- natural sciences mathematics pure mathematics mathematical analysis functional analysis
- natural sciences biological sciences molecular biology structural biology
You need to log in or register to use this function
We are sorry... an unexpected error occurred during execution.
You need to be authenticated. Your session might have expired.
Thank you for your feedback. You will soon receive an email to confirm the submission. If you have selected to be notified about the reporting status, you will also be contacted when the reporting status will change.
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-IRG-2008
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
84105 Beer Sheva
Israel
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.