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Quantum Dot-Based Highly Sensitive Immunoassays for Multiplexed Diagnostics of Alzheimer's Disease

Objective

6.1 million people currently live with a form of dementia in the European Union with an addition of 1.4 million new cases every year. Combination of psychological testing, brain-imaging and exclusion of other neurological disorders makes the diagnosis of Alzheimer’s disease complicated and time consuming (taking up to 20 months). A rapid, sensitive and specific immunoassay for protein markers inside blood would largely improve early diagnosis and lead to a better treatment of dementia. Homogeneous assays based on FRET from one dye labeled specific antibody (AB1) to another (AB2) within an “AB1-biomarker-AB2” immune complex are an ideal basis to meet these diagnostic requirements. As the detection of several protein markers is obligatory for a highly sensitive and specific diagnosis an optical multiplexing approach with dyes of different colors is a smart solution. Semiconductor quantum dots (QDs) are the ideal candidates due to their size-dependent absorption and emission wavelengths. Moreover, they possess unique photophysical properties that overcome conventional fluorescence dyes. In combination with lanthanide complexes (LCs), that display long luminescence lifetimes and well separated emission bands, QDs render a powerful multiplexing tool for highly sensitive diagnostics even for large immune complexes. FRET applications using QDs are to date restricted to academic research and a profound understanding of QD-based FRET is not available. For a comprehensive analysis the use of LCs is mandatory, because they are the only known donors for efficient FRET to QD acceptors. NANOGNOSTICS strives for a profound understanding of QD-based FRET, the synthetic creation of highly efficient QD immune sensors for detection of several Alzheimer-specific protein markers and the development of a modular high-throughput-screening immuno analyzer for the integration of QD-based multiplexing immunoassays into early diagnosis for improved patient outcome in dementia therapy.

Field of science

  • /natural sciences/physical sciences/electromagnetism and electronics/electrical conductivity/semiconductor
  • /engineering and technology/materials engineering/colors
  • /medical and health sciences/basic medicine/neurology/alzheimer

Call for proposal

FP7-HEALTH-2009-single-stage
See other projects for this call

Funding Scheme

CP-FP - Small or medium-scale focused research project

Coordinator

FRAUNHOFER GESELLSCHAFT ZUR FOERDERUNG DER ANGEWANDTEN FORSCHUNG E.V.
Address
Hansastrasse 27C
80686 Munchen
Germany
Activity type
Research Organisations
EU contribution
€ 448 725
Administrative Contact
Walter Krause (Mr.)

Participants (9)

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
France
EU contribution
€ 578 800
Address
Rue Michel Ange 3
75794 Paris
Activity type
Research Organisations
Administrative Contact
Denis Fix (Mr.)
UNIVERSITAET POTSDAM
Germany
EU contribution
€ 364 800
Address
Am Neuen Palais 10
14469 Potsdam
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Regina Gerber (Dr.)
PHILIPPS UNIVERSITAET MARBURG
Germany
EU contribution
€ 453 500
Address
Biegenstrasse 10
35037 Marburg
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Wolfgang Parak (Prof.)
THE HEBREW UNIVERSITY OF JERUSALEM
Israel
EU contribution
€ 515 100
Address
Edmond J Safra Campus Givat Ram
91904 Jerusalem
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Jane Turner (Ms.)
CHARITE - UNIVERSITAETSMEDIZIN BERLIN
Germany
EU contribution
€ 488 700
Address
Chariteplatz 1
10117 Berlin
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Eveline Fräßdorf (Ms.)
TURUN YLIOPISTO
Finland
EU contribution
€ 373 599,80
Address
Yliopistonmaki
20014 Turku
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Pekka Hänninen (Prof.)
EDINBURGH INSTRUMENTS LTD
United Kingdom
EU contribution
€ 539 400
Address
Bain Square 2
EH54 7DQ Livingston
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
David Mccarlie (Mr.)
Arctic Photonics Oy

Participation ended

Finland
Address
Tillinmaanpuisto 3A
02330 Espoo
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Scott Buchter (Dr.)
UNIVERSITE PARIS-SUD
France
EU contribution
€ 274 439,20
Address
Rue Georges Clemenceau 15
91405 Orsay Cedex
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Nicolas Lecompte (Mr.)