Final Report Summary - EPINCRNA (Epigenetic and genome stability: non coding RNA-mediated chromatin modifications in S. cerevisiae)
With this project, we draw the first exhaustive catalog of yeast antisense lncRNA using original High throughput technologies and bioinformatics pipelines. Second, we described further mechanistic insights into the functional role of antisense lncRNA on genome expression and stability. Our data showed the existence of an entire class of antisense regulatory lncRNA extremely sensitive to RNA decay pathways, impinging their study so far. The roles for yeast antisense lncRNAs in shaping the epigenome paved the way for future fundamental studies: We propose that the dark side of the non-coding genome is another layer of gene regulation complexity that needs to be deciphered.