Objetivo In eukaryotic cells the DNA is packaged into nucleosomes and higher order structures which lead to a condensation and protection of the DNA. During important cellular processes such as transcription or replication access to the DNA has to be granted. This is facilitated by ATP dependent nucleosome remodelling. The mechanistic details how the involved remodelling complexes succeed in providing access to the nucleosomal DNA are currently in spite of large experimental efforts not well understood. The aim of the proposal is to unravel the molecular mechanism of nucleosome remodelling using single-molecule fluorescence resonance energy transfer (FRET). By putting labels on the nucleosomal DNA, the histones or the remodellers we will - step by step - determine the conformational changes that occur during remodelling and use this information to build a mechanistic model. We will also use the controlled assembly of 30 nm fibers from purified components in order to determine how remodelling occurs in this structurally restricted environment. The large size of the chromatin fibers will dictate that in addition to FRET measurements, which will again be used to investigate local motion, super-resolution microscopy need to be employed to obtain information about long-distance movements. To this end we will use stochastic optical reconstruction microscopy (STORM), which allows for accuracy below 10 nm, thus ideally complementing the FRET approach. In summary our experiments will lead us to a mechanistic understanding of ATP dependent nucleosome remodelling, both on mono-nucleosomes as well as in higher order structures. This knowledge will in return stimulate new initiatives aimed at understanding the nature and regulation of chromatin dynamics in vivo. Ámbito científico ingeniería y tecnologíaingeniería de materialesfibrasciencias naturalesciencias biológicasgenéticaADNciencias naturalesciencias físicasópticamicroscopíamicroscopía de superresolución Palabras clave DNA FRET STED STORM chromatin fluorescence live cell imaging molecular motor remodelling single-molecule Programa(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Tema(s) ERC-SG-LS1 - ERC Starting Grant - Molecular and Structural Biology and Biochemistry Convocatoria de propuestas ERC-2009-StG Consulte otros proyectos de esta convocatoria Régimen de financiación ERC-SG - ERC Starting Grant Coordinador UNIVERSITAET ULM Dirección Helmholtzstrasse 16 89081 Ulm Alemania Ver en el mapa Región Baden-Württemberg Tübingen Ulm, Stadtkreis Tipo de actividad Higher or Secondary Education Establishments Investigador principal Jens Michaelis (Prof.) Contacto administrativo Dieter Kaufmann (Mr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Aportación de la UE Sin datos Beneficiarios (2) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo UNIVERSITAET ULM Alemania Aportación de la UE € 869 062,00 Dirección Helmholtzstrasse 16 89081 Ulm Ver en el mapa Región Baden-Württemberg Tübingen Ulm, Stadtkreis Tipo de actividad Higher or Secondary Education Establishments Investigador principal Jens Michaelis (Prof.) Contacto administrativo Dieter Kaufmann (Mr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Otras fuentes de financiación Sin datos LUDWIG-MAXIMILIANS-UNIVERSITAET MUENCHEN La participación finalizó Alemania Aportación de la UE € 526 319,00 Dirección Geschwister scholl platz 1 80539 Muenchen Ver en el mapa Región Bayern Oberbayern München, Kreisfreie Stadt Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Helmut Eckl (Mr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Otras fuentes de financiación Sin datos
