Obiettivo In eukaryotic cells the DNA is packaged into nucleosomes and higher order structures which lead to a condensation and protection of the DNA. During important cellular processes such as transcription or replication access to the DNA has to be granted. This is facilitated by ATP dependent nucleosome remodelling. The mechanistic details how the involved remodelling complexes succeed in providing access to the nucleosomal DNA are currently in spite of large experimental efforts not well understood. The aim of the proposal is to unravel the molecular mechanism of nucleosome remodelling using single-molecule fluorescence resonance energy transfer (FRET). By putting labels on the nucleosomal DNA, the histones or the remodellers we will - step by step - determine the conformational changes that occur during remodelling and use this information to build a mechanistic model. We will also use the controlled assembly of 30 nm fibers from purified components in order to determine how remodelling occurs in this structurally restricted environment. The large size of the chromatin fibers will dictate that in addition to FRET measurements, which will again be used to investigate local motion, super-resolution microscopy need to be employed to obtain information about long-distance movements. To this end we will use stochastic optical reconstruction microscopy (STORM), which allows for accuracy below 10 nm, thus ideally complementing the FRET approach. In summary our experiments will lead us to a mechanistic understanding of ATP dependent nucleosome remodelling, both on mono-nucleosomes as well as in higher order structures. This knowledge will in return stimulate new initiatives aimed at understanding the nature and regulation of chromatin dynamics in vivo. Campo scientifico ingegneria e tecnologiaingegneria dei materialifibrescienze naturaliscienze biologichegeneticaDNAscienze naturaliscienze fisicheotticamicroscopiamicroscopia a super risoluzione Parole chiave DNA FRET STED STORM chromatin fluorescence live cell imaging molecular motor remodelling single-molecule Programma(i) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Argomento(i) ERC-SG-LS1 - ERC Starting Grant - Molecular and Structural Biology and Biochemistry Invito a presentare proposte ERC-2009-StG Vedi altri progetti per questo bando Meccanismo di finanziamento ERC-SG - ERC Starting Grant Coordinatore UNIVERSITAET ULM Indirizzo Helmholtzstrasse 16 89081 Ulm Germania Mostra sulla mappa Regione Baden-Württemberg Tübingen Ulm, Stadtkreis Tipo di attività Higher or Secondary Education Establishments Ricercatore principale Jens Michaelis (Prof.) Contatto amministrativo Dieter Kaufmann (Mr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Contributo UE Nessun dato Beneficiari (2) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto UNIVERSITAET ULM Germania Contributo UE € 869 062,00 Indirizzo Helmholtzstrasse 16 89081 Ulm Mostra sulla mappa Regione Baden-Württemberg Tübingen Ulm, Stadtkreis Tipo di attività Higher or Secondary Education Establishments Ricercatore principale Jens Michaelis (Prof.) Contatto amministrativo Dieter Kaufmann (Mr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Altri finanziamenti Nessun dato LUDWIG-MAXIMILIANS-UNIVERSITAET MUENCHEN Partecipazione conclusa Germania Contributo UE € 526 319,00 Indirizzo Geschwister scholl platz 1 80539 Muenchen Mostra sulla mappa Regione Bayern Oberbayern München, Kreisfreie Stadt Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo Helmut Eckl (Mr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Altri finanziamenti Nessun dato
