Final Report Summary - EURIBIO (Dissecting the biogenesis of eukaryotic ribosomal subunits)
By employing diverse approaches in budding yeast, in combination with quantitative proteomic and integrative structural biology approaches, our research has revealed three different aspects of eukaryotic ribosome assembly:
(1) The uncharted interface between nucleo-cytoplasmic transport machinery and the ribosome assembly pathway.
(2) Compositional dynamics of pre-ribosomal particles as they travel from the nucleolus to the cytoplasm.
(3) Quality control steps that prevent incorrectly assembled pre-ribosomes from initiating translation.
Our analyses generated principles by which large complexes are assembled/disassembled, checked and targeted in vivo.