Objective Fast developments in synthetic biology now enable introduction of entire novel-to-nature pathways into industrial microorganisms, thus opening the door to a novel bio-based industry. However, successful scale-up of novel-to-market products from bench-top proof-of-principle to full-scale processes requires a profound understanding of cellular and sub-cellular mechanisms and their interaction with industrial process conditions. In particular, successful industrial production requires optimal performance of engineered strains under the dynamic, slow-growth process conditions that occur in large-scale industrial bioreactors. These differ strongly from the conditions in conventional laboratory analysis.The RoBoYeast project aims to reduce time-to-market and to improve productivity of microbial synthetic pathways by identifying key physiological and molecular determinants of performance under industrially relevant conditions and to integrate these at an early stage in strain development programs. The project is characterized by multi-scale analysis and integration of results from industrial-scale into lab-scale studies.RoBoYeast aims to improve productivity and cellular robustness in large-scale fermentations of the novel-to-nature production in Saccharomyces cerevisiae of arachidonic and eicosapentaenoic acid, two health-promoting poly-unsaturated fatty acids with excellent market perspectives as nutraceuticals. Biosynthetic pathways of these compounds are energy (ATP) intensive, which adds on the challenge of constructing robust strains that withstand typical production stresses without loss of productivity and product yield. To accelerate project progress, key findings of a likewise challenging resveratrol progress, already developed by the SME partner, can be used. RoBoYeast intends to identify and improve robustness mechanism not only for the benefit of this project but also as a blueprint for optimization of the production of other ATP-intensive products. Fields of science engineering and technologyenvironmental biotechnologybioremediationbioreactorsnatural sciencesbiological sciencessynthetic biologynatural sciencesbiological sciencesbiochemistrybiomoleculeslipidssocial scienceseconomics and businesseconomicsproduction economicsproductivityengineering and technologyindustrial biotechnologybioprocessing technologiesfermentation Programme(s) FP7-KBBE - Specific Programme "Cooperation": Food, Agriculture and Biotechnology Topic(s) KBBE.2011.3.3-03 - Cellular, metabolic and genetic engineering for novel compounds Call for proposal FP7-KBBE-2011-5 See other projects for this call Funding Scheme CP-TP - Collaborative Project targeted to a special group (such as SMEs) Coordinator UNIVERSITY OF STUTTGART EU contribution € 709 270,00 Address KEPLERSTRASSE 7 70174 Stuttgart Germany See on map Region Baden-Württemberg Stuttgart Stuttgart, Stadtkreis Activity type Higher or Secondary Education Establishments Administrative Contact Ralf Takors (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data Participants (2) Sort alphabetically Sort by EU Contribution Expand all Collapse all TECHNISCHE UNIVERSITEIT DELFT Netherlands EU contribution € 834 958,00 Address STEVINWEG 1 2628 CN Delft See on map Region West-Nederland Zuid-Holland Delft en Westland Activity type Higher or Secondary Education Establishments Administrative Contact Rogier Van Loghem Links Contact the organisation Opens in new window Website Opens in new window Total cost No data FLUXOME SCIENCES A/S Denmark EU contribution € 527 119,00 Address DIPLOMVEJ 378 2800 LYNGBY See on map Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Sarah Barrett (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data