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Contenuto archiviato il 2024-06-18

Interactions in Three-dimensional (3D) in vitro environments of Nanoparticles and Cells

Obiettivo

The applications for nanoparticles (NPs) in medicine have rapidly increased in recent years; some examples are drug delivery, medical imaging, in vitro biosensors and cancer treatment. Typically, the biological assessment of NPs is carried out first in vitro (in a 2D petri dish) and then in small animals in vivo (3D). This whole process is costly and time consuming; similarly to that is required for the drug development, 11-15 years and $500 to $800 million to reach the market. An intermediate step between (2D) in vitro and (3D) in vivo assays could provide relevant information, decreasing the total cost of the biological assessment. 3D in vitro assays mimic biologically relevant tissues in an economical and rapid manner; although, current 3D in vitro methods entail complex engineering steps. 3DinvitroNPC focuses on a simple and innovative strategy to create 3D in vitro cell cultures using sheets of porous materials and embedding living cells and NPs within those materials. 3D cell cultures require a material non-toxic for cells and highly porous to allow cells to grow within its microstructure. This project explores aerogels of biomaterials to create 3D in vitro novel scaffolds that are biodegradable and transparent. Combinations of cells and NPs in the 3D in vitro constructs permits the assessment of viability and functionality of NPs in relevant biological environments which is critical for using NPs in medical applications. Gold NPs and FexOy-NPs will be assessed, the first as model NPs and the latter as magnetic label of cells. FexOy-NPs internalized by cells, magnetically label the cells. Localization of magnetic cells using a magnet placed outside the body is a recently developed medical method non-invasive for tissue recovery after cerebral ischemia. For this purpose, the platform of 3DinvitroNPC will evaluate magnetic cells within the on-purpose developed 3D cell scaffolds.

Campo scientifico (EuroSciVoc)

Argomento(i)

Invito a presentare proposte

FP7-PEOPLE-2011-CIG
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Meccanismo di finanziamento

MC-CIG - Support for training and career development of researcher (CIG)

Coordinatore

AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Contributo UE
€ 100 000,00
Indirizzo
CALLE SERRANO 117
28006 Madrid
Spagna
Regione
Comunidad de Madrid Comunidad de Madrid Madrid
Tipo di attività
Research Organisations
Collegamenti
Costo totale
Nessun dato