Obiettivo During meiosis, homologous recombination plays a mechanical role by connecting homologous chromosomes, thus allowing proper chromosome segregation during the first meiotic division. In most species, the absence of meiotic recombination leads to sterility. In addition, recombination generates new combinations of alleles, increases genome diversity and plays a major role in genome evolution.Meiotic recombination is initiated by the programmed induction of DNA double-strand breaks (DSBs), but how these events are controlled at the molecular level and how they are constrained by selective pressures during evolution is not understood.Our recent historical discovery that the Prdm9 gene controls the localization of recombination in the mouse and human genomes revolutionizes our view on this process, with one main unanticipated finding: the highly dynamic and fast evolution of Prdm9 and of meiotic DSB sites in the genome. Understanding meiotic recombination clearly requires the development of novel approaches to analyze this process from both molecular and evolutionary perspectives.To this aim, we will develop an extensive analysis of Prdm9 as its activity, role, regulators and sites of action in the genome need to be identified and understood in order to gain insight into its dynamics and evolution.We will develop a unique and challenging strategy to overcome the limitation of laboratory mice and pioneer the analysis of Prdm9 and recombination activity in wild mice.We thus aim at making a breakthrough in the field by bringing molecular genetics and evolutionary biology together, to grasp the significance of meiotic recombination for genome evolution and sexual reproduction in eukaryotes. Campo scientifico natural sciencesbiological sciencesmolecular biologymolecular geneticsnatural sciencesbiological sciencesevolutionary biologynatural sciencesbiological sciencesgeneticsheredity Programma(i) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Argomento(i) ERC-AG-LS1 - ERC Advanced Grant - Molecular and Structural Biology and Biochemistry Invito a presentare proposte ERC-2012-ADG_20120314 Vedi altri progetti per questo bando Meccanismo di finanziamento ERC-AG - ERC Advanced Grant Istituzione ospitante CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS Contributo UE € 2 491 899,00 Indirizzo RUE MICHEL ANGE 3 75794 Paris Francia Mostra sulla mappa Regione Ile-de-France Ile-de-France Paris Tipo di attività Research Organisations Ricercatore principale Bernard Robert De Massy (Dr.) Contatto amministrativo Jocelyn Mere (Mr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato Beneficiari (1) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS Francia Contributo UE € 2 491 899,00 Indirizzo RUE MICHEL ANGE 3 75794 Paris Mostra sulla mappa Regione Ile-de-France Ile-de-France Paris Tipo di attività Research Organisations Ricercatore principale Bernard Robert De Massy (Dr.) Contatto amministrativo Jocelyn Mere (Mr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato