Objective
"The Trans Golgi Network (TGN) is the central sorting station for newly synthesised proteins in the cell. Similar to a postal distribution
center, incoming cargo is selected, sorted, and packaged to be carried to its final cellular destination by the postman in charge. Due to the
involvement of numerous different cargoes and pleiomorhic carriers for the different destinations this process of protein sorting at the TGN
is highly sophisticated. While the sorting event of lysosomal hydrolases at the TGN via mannose-6-phosphate receptor and clathrin-coated
vesicles to the lysosome is well characterised, the relevant mechanism for other luminal proteins at the TGN remains elusive.
We recently discovered a new role for the actin severing protein twinstar in Drosophila and its orthologs in yeast (cof1) and mammalian
cells (ADF and cofilin1) in regulation of luminal protein sorting at the TGN. This process is a novel calcium dependent sorting mechanism
that directly links the actin cytoskeleton and ADF/cofilin to the TGN localized P-Type calcium ATPase SPCA1.
In the project described herein we will characterize the new sorting mechanism in detail. New biochemical tools will identify components of
the sorting machinery on both cytosolic and luminal faces of the TGN. Cutting edge Mass Spectrometry will dissect the composition of TGN
associated or luminal protein complexes. FLIM experiments will elucidate the temporal and spatial dynamics of the complexes. High
throughput screening in yeast will yield new components on a genome wide level. Functional assays utilising siRNA and reconstitution will
define their precise role in cargo sorting in vitro and in vivo. The complementary investigation of a Drosophila model will elucidate the
physiological relevance of the process in the intact organism and its impact on developmental biology. Altogether, this work represents an
essential experimental set up to answer a yet completely unresolved question in cell biology."
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences chemical sciences inorganic chemistry alkaline earth metals
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences cell biology
- natural sciences biological sciences developmental biology
- natural sciences chemical sciences analytical chemistry mass spectrometry
You need to log in or register to use this function
We are sorry... an unexpected error occurred during execution.
You need to be authenticated. Your session might have expired.
Thank you for your feedback. You will soon receive an email to confirm the submission. If you have selected to be notified about the reporting status, you will also be contacted when the reporting status will change.
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2012-CIG
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MC-CIG - Support for training and career development of researcher (CIG)
Coordinator
80539 Munchen
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.