Objetivo .Mammalian transcriptome analysis reveals that while most of the genome is transcribed, only a minority fraction produces protein coding, messenger RNA or functional, structural RNA. Instead a substantial fraction is transcribed, often tissue specifically, into long non coding (lnc)RNA of mostly unknown function. Many lncRNA are rapidly degraded and may reflect promiscuous, non-functional transcription. However some lncRNA have important cellular functions. I reason that defining promoters and terminators of lncRNA genes will provide valuable information on which lncRNA are likely to be functional. LncRNA gene promoters often correspond to nucleosome depleted regions of protein coding gene promoters and enhancers, which generate bidirectional transcription. Regions of single-strand DNA, exposed by R-loop formation can also initiate transcription and so act as a potential source of lncRNA, as will be investigated here. Since transcription initiation of lncRNA genes appears relatively promiscuous, terminating lncRNA transcription may be of critical importance. This will prevent read-through transcription with consequent negative affects on downstream genes by transcriptional interference or the formation of overlapping transcripts leading to RNA interference effects. Consequently I will focus on transcription termination mechanisms of lncRNA genes. I will determine if known protein coding gene terminators also operate for lncRNA genes or whether many lncRNA use alternative termination mechanisms. In particular I will investigate the role of G-rich pausing elements that promote Pol II termination by forming R-loop structures on lncRNA genes. I will also investigate which classes of lncRNA genes form gene loop conformations when transcribed, as a likely identifier of more highly expressed, functional lncRNA. Overall I predict that understanding how lncRNA are synthesised will provide critical information on which lncRNA warrant scrutiny as likely functional transcripts Ámbito científico natural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesgeneticsRNAnatural sciencesbiological sciencesgeneticsgenomes Programa(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Tema(s) ERC-AG-LS1 - ERC Advanced Grant - Molecular and Structural Biology and Biochemistry Convocatoria de propuestas ERC-2013-ADG Consulte otros proyectos de esta convocatoria Régimen de financiación ERC-AG - ERC Advanced Grant Institución de acogida THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD Aportación de la UE € 2 499 464,00 Dirección WELLINGTON SQUARE UNIVERSITY OFFICES OX1 2JD Oxford Reino Unido Ver en el mapa Región South East (England) Berkshire, Buckinghamshire and Oxfordshire Oxfordshire Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Gill Wells (Ms.) Investigador principal Nicholas Jarvis Proudfoot (Prof.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos Beneficiarios (1) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD Reino Unido Aportación de la UE € 2 499 464,00 Dirección WELLINGTON SQUARE UNIVERSITY OFFICES OX1 2JD Oxford Ver en el mapa Región South East (England) Berkshire, Buckinghamshire and Oxfordshire Oxfordshire Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Gill Wells (Ms.) Investigador principal Nicholas Jarvis Proudfoot (Prof.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos