Objective
This proposal aims at determining the role of planktonic ammonia-oxidizing archaea (AOA) versus ammonia-oxidizing bacteria (AOB) in the marine pelagic environments that are critical for global nitrogen cycle. This will be accomplished by using a combination of conventional enrichment techniques with recently developed molecular methods.
By means of a modified catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH) method and quantitative PCR technology, the abundance of the major prokaryotic groups of ammonia-oxidizers (AOB and AOA) will be determined in two coastal ecosystems (Irish and Black Sea) as compared to meso- and bathypelagic deep water of the Atlantic ocean.
Combining this method with micro-autoradiography we will be able to determine inorganic N uptake of individual cells and relate this to the measurements on bulk archaeal and prokaryotic community.
AOB and AOA distribution and diversity will be determined via terminal-restriction fragment polymorphism analysis of ammonia monooxygenase (amoA) genes combined with construction of environmental DNA bank for each sampling site.
Specific new oligonucleotide probes for dominant ammonia-species will be designed and applied in the MICRO-FISH assay to determine interspecific differences in the utilization and activity of coastal and deep water ammonia-oxidizers.
Seawater batch cultures with coastal and deep ocean water will be used to isolate new AOA and AOB species using a laboratory enrichment and cultivation approach. The obtained pure culture and environmental samples will be used for quantitative analysis of archaeal and bacterial nitrification amoA and denitrification nirK and norB gene expression in microcosms experiments.
While the AOB and AOA genes and enzymes involved in ammonia oxidation and N2O production have been recently isolated, the extent to which AOA and AOB are actively oxidizing ammonia and/or producing N2O under varying environmental conditions has never been studied before.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences chemical sciences electrochemistry electrolysis
- natural sciences physical sciences optics microscopy
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
- natural sciences biological sciences ecology ecosystems coastal ecosystems
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2005-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
7ZX LIVERPOOL
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.