Objective
The objective of this project is to elucidate the molecular mechanisms underlying a novel pathway for the endocytosis and intracellular trafficking of caveolae. It has recently become clear that molecules partitioning in lipid rafts do not always follow the classical clathrin-mediated pathway but can be internalized via a distinct caveolae-mediated route. First, I will develop fluorescence microscopy-based assays to analyze the intracellular trafficking and dynamics of caveolae upon internalization from the plasma membrane. Second, I will design a screening assay to identify key molecules involved in caveolae-mediated endocytosis by targeted, genome-wide RNA interference. Complementary, proteomics will be performed on isolated endocytic intermediates. We have the following aims:
1. To develop assays, using new light microscopy techniques that allow us to visualize and quantify the internalization and trafficking of individual caveolae in living cells.
2. To obtain a list of genes functionally involved in caveolae-mediated endocytosis, using genome-wide RNA interference screening.
3. To isolate caveosomes, to determine their constituents by proteomics analysis, and to design in vitro membrane traffic assays.
4. To obtain putative functions of the molecules identified in 2) and 3) with systematic database mining, sequence alignments and protein domain searches, and to use in vitro and in vivo systems established in 1) and 3) to test these predicted functions directly. This will provide complementary training and will generate data on which an independent research career in the field of endocytosis can be based. The screens will provide a proof of concept for the research community and novel insights into yet uncharacterised membrane traffic events. A better understanding is important because these pathways are involved in basic cell signaling, lipid homeostasis and are used by several viruses, pathogenic bacteria and bacterial toxins.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences biological sciences cell biology cell signaling
- natural sciences biological sciences biochemistry biomolecules lipids
- natural sciences mathematics pure mathematics mathematical analysis functional analysis
- natural sciences biological sciences genetics RNA
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
DRESDEN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.