Objective
Because changes in the organization and composition of extra-cellular matrix can have consequences on mechanical properties of the arterial wall, understand mechanisms of maturation of the elastin and collagen, (maines extra-cellular proteins) is important.
Vascular smooth muscle cells highly express vascular adhesion protein 1 (VAP-1), which display a semicarbazide-amine oxidase (SSAO) activity. Studies using pharmacological inhibitors of SSAO have been previously suggested that SSAO could be important for the arterial wall organization, without excluding that a part of the affects was due to lysyl oxidase inhibition. Lysyl oxydase is the main enzyme establishing cross-links in or between elastin and collagan until now.So I will study tha SSAO role in arterial wall, comparing arteries from wild type, VAP-1 knockout mice (establishment by the host) and VAP-1 knouckhout mice over-expressing VAP-1 in smooth muscles (will established during this project).
Histomorphometry and immunohistochemistry will be used to evaluate the arterial morphology and organization, content in elastin and collagen will also be measured. Arterial mechanical properties will be evaluated in vivo with eckotracking and mechanical resistance will be tested in vitro with the collaboration of Lacolley's laboratory. Moreover, because endogenous substrate of VAP-1/SSAO are not known, I will search whether SSAO from vascular smooth muscle cells is able to oxidize directly amine moiety in elastin and collagen to cross-link these molecules.
Then, I will verify whether SSAO activity can regulate vascular smooth muscle cells phenotype and expression of extra-cellular matrix proteins. VAP-1/SSAO expression will be measured in function of quality and quantity of extra-cellular matric
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences clinical medicine angiology vascular diseases
- medical and health sciences clinical medicine cardiology cardiovascular diseases arteriosclerosis
- medical and health sciences basic medicine physiology
- natural sciences chemical sciences organic chemistry amines
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
TURKU
Finland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.