Role of VAP-1/SSAO in arterial wall physiology

Projektinformationen

VAP1 IN ARTERIES

ID Finanzhilfevereinbarung: 515992

Projekt abgeschlossen

Startdatum 1 Mai 2005

Enddatum 30 April 2007

Finanziert unter

• Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006

Gesamtkosten € 0,00

EU-Beitrag € 164 267,00

Koordiniert durch

UNIVERSITY OF TURKU
Finland

Ziel

Because changes in the organization and composition of extra-cellular matrix can have consequences on mechanical properties of the arterial wall, understand mechanisms of maturation of the elastin and collagen, (maines extra-cellular proteins) is important.

Vascular smooth muscle cells highly express vascular adhesion protein 1 (VAP-1), which display a semicarbazide-amine oxidase (SSAO) activity. Studies using pharmacological inhibitors of SSAO have been previously suggested that SSAO could be important for the arterial wall organization, without excluding that a part of the affects was due to lysyl oxidase inhibition. Lysyl oxydase is the main enzyme establishing cross-links in or between elastin and collagan until now.So I will study tha SSAO role in arterial wall, comparing arteries from wild type, VAP-1 knockout mice (establishment by the host) and VAP-1 knouckhout mice over-expressing VAP-1 in smooth muscles (will established during this project).

Histomorphometry and immunohistochemistry will be used to evaluate the arterial morphology and organization, content in elastin and collagen will also be measured. Arterial mechanical properties will be evaluated in vivo with eckotracking and mechanical resistance will be tested in vitro with the collaboration of Lacolley's laboratory. Moreover, because endogenous substrate of VAP-1/SSAO are not known, I will search whether SSAO from vascular smooth muscle cells is able to oxidize directly amine moiety in elastin and collagen to cross-link these molecules.

Then, I will verify whether SSAO activity can regulate vascular smooth muscle cells phenotype and expression of extra-cellular matrix proteins. VAP-1/SSAO expression will be measured in function of quality and quantity of extra-cellular matric

Wissenschaftliches Gebiet

• medical and health sciences › clinical medicine › angiology › vascular diseases
• medical and health sciences › clinical medicine › cardiology › cardiovascular diseases › arteriosclerosis
• medical and health sciences › basic medicine › physiology
• natural sciences › chemical sciences › organic chemistry › amines
• natural sciences › biological sciences › biochemistry › biomolecules › proteins › enzymes

Schlüsselbegriffe

• Amine oxidase
• VAP1 knockout mice
• cell differentiation
• elastin
• extracellular matrix
• gene expression
• hydrogen peroxide
• mechanical properties of arteries
• vascular smooth musle cells

Programm/Programme

• FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006

Thema/Themen

• MOBILITY-2.1 - Marie Curie Intra-European Fellowships (EIF)

Aufforderung zur Vorschlagseinreichung

FP6-2002-MOBILITY-5
Andere Projekte für diesen Aufruf anzeigen

Finanzierungsplan

EIF - Marie Curie actions-Intra-European Fellowships

Koordinator

UNIVERSITY OF TURKU

• EU-Beitrag: Keine Daten
• Adresse: Tykistokatu 6A ; TURKU ; Finnland
• Gesamtkosten: Keine Daten