Ziel
The DLK1-GTL2 domain is a recently discovered,evolutionary served,H 1 Mb cluster of imprinted genes mapping to 14q32 in man,distal 12 in the mouse and distal 18q in sheep.It tains at least four protein encoding genes expressed from the paternal allele (DLK1,RTL1,DIO3 and BEGAIN),as well as a series of non coding RNA genes expressed from the maternal allele (e.g. GTL2, MEG8, MIRG).It was recently demonstrated that the DLK1-GTL2 domain tains multiple small non coding RNA genes.More specifically,it includes (i) a pair of miRNA genes (miR-127 and miR-136) antisense to RTL1, (ii) a clusters of H C/D snoRNA genes thought to be processed from the introns of the non coding MEG8 gene,and (iii) a cluster of H 40 miRNA genes thought to be processed from a large precursor transcript referred to as MIRG. Elucidating the biological function and mode of action of the miRNA genes in the DLK1-GTL2 domain is the topic of this proposal.More precisely,the objectives of the Callimir proposal are:(i) to study the role of the miR-127 and miR-136 genes in development and in regulating the expression of the RTL1 gene and (ii) to study the role of the MIRG cluster of miRNA genes in development and in mediating the trans effect underlying polar overdominance.
Wissenschaftliches Gebiet
Schlüsselbegriffe
Programm/Programme
Aufforderung zur Vorschlagseinreichung
FP6-2003-LIFESCIHEALTH-I
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