Objective Dynamic signalling networks in Diabetic Nephropathy (DN) – New avenues to a personalized therapy.-We have developed an exquisite experimental platform that facilitates the systematic unravelling of the signallingnetworks leading to (1) the initiation, (2) the progression and (3) the potential regeneration of podocytes inDN, paving the way to novel therapeutic strategies:(1) DN initiation: Identification of signalling cascades leading to microalbuminuria: MolecularBy combining transgenic Drosophila lines carrying secreted fluorescent proteins to monitor the barrier functionin vivo with a genome-wide siRNA screen we will establish a unique system to directly identify genenetworks contributing to microalbuminuria.(2a) DN progression: Molecular fingerprinting of podocyte degeneration: Based on a transgenicfluorescent mouse model, we have pioneered a highly efficient podocyte purification method from type1 andtype 2 diabetic mice allowing us to develop a precise molecular genetic, quantitative proteomic and microRNA fingerprint from freshly isolated podocytes from diabetic and non-diabetic mice.(2b) DN progression: We established a proteomic approach to measure site-specific phosphorylation dynamics inprimary podocyte cultures originating from transgenic mice that are TORC1 deficient, TORC2 deficient orTORC1 hyperactive (TSC1 KO) solely in the podocytes.(3) Potential role of podocyte regeneration in DN: Finally, to target mechanisms that could potentiallyreverse the disease process (by repopulating lost podocytes), we invented a strategy to quantitatively monitorpodocyte turnover from different stem cell niches allowing us to precisely assess and potentiallymanipulating the capacity of podocyte regeneration in DN. Fields of science medical and health sciencesclinical medicineendocrinologydiabetesdiabetic nephropathynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsmedical and health sciencesmedical biotechnologycells technologiesstem cellsnatural sciencesbiological sciencesgeneticsRNA Programme(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) ERC-CG-2013-LS4 - ERC Consolidator Grant - Physiology, Pathophysiology and Endocrinology Call for proposal ERC-2013-CoG See other projects for this call Funding Scheme ERC-CG - ERC Consolidator Grants Host institution UNIVERSITAETSKLINIKUM HAMBURG-EPPENDORF EU contribution € 740 431,50 Address Martinistrasse 52 20251 Hamburg Germany See on map Region Hamburg Hamburg Hamburg Activity type Higher or Secondary Education Establishments Administrative Contact Tobias Huber (Prof.) Principal investigator Tobias Georg Bruno Maria Huber (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data Beneficiaries (2) Sort alphabetically Sort by EU Contribution Expand all Collapse all UNIVERSITAETSKLINIKUM HAMBURG-EPPENDORF Germany EU contribution € 740 431,50 Address Martinistrasse 52 20251 Hamburg See on map Region Hamburg Hamburg Hamburg Activity type Higher or Secondary Education Establishments Administrative Contact Tobias Huber (Prof.) Principal investigator Tobias Georg Bruno Maria Huber (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data UNIVERSITAETSKLINIKUM FREIBURG Participation ended Germany EU contribution € 1 259 488,50 Address HUGSTETTER STRASSE 49 79106 Freiburg See on map Region Baden-Württemberg Freiburg Freiburg im Breisgau, Stadtkreis Activity type Higher or Secondary Education Establishments Administrative Contact Maren Mihlan (Mrs.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data