Objective Arterial calcifications, like those seen in patients with atherosclerosis, contribute to morbidity and are predictors of premature death. Despite exposure to the same cardiovascular risk factors, previous studies have demonstrated the concept of the heterogeneity of atheroma according to the arterial beds. These differences have a major clinical impact on the occurrence of cardiovascular events (embolism, thrombosis, dissection, restenosis), and recurrence of the disease. The host laboratory recently showed that plaque heterogeneity was notably associated with a difference in calcification burden. Further classification distinguished amorphous from cartilage or even bone structures in the affected vasculature. This process remains poorly understood.This project aims to identify cellular and molecular mechanisms involved in the arterial calcification heterogeneity, by first fully characterizing the lesions in various vascular beds and by associating specific features of the plaque with calcification content. I will also test in vitro the contribution of endothelial cells (EC) and pericytes in vascular cells mineralization and calcification. In particular, I will assess the ability of EC and pericytes to alter macrophage and smooth muscle cell differentiation towards an osteoblast-like cell phenotype. The role of specific molecular pathways (RANK/RANL/OPG...) will be also tested functionally. To further analyze this process in vivo, I will generate double KO mice developing accelerated calcification in lesions and use in vivo fluorescence imaging to monitor plaque formation and calcification over time. Finally, I will assess the predictive clinical impact of the nature of the plaque on intra-stent restenosis in patients following OCT and serial duplex ultrasound imaging.Altogether, this project should greatly contribute to the characterization of the molecular basis of arterial calcification and the difference in bone metabolism in various vascular beds. Fields of science medical and health sciencesclinical medicineangiologyvascular diseasesmedical and health sciencesclinical medicinecardiologycardiovascular diseasesarteriosclerosisnatural sciencesphysical sciencesacousticsultrasound Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) FP7-PEOPLE-2013-IIF - Marie Curie Action: "International Incoming Fellowships" Call for proposal FP7-PEOPLE-2013-IIF See other projects for this call Funding Scheme MC-IIF - International Incoming Fellowships (IIF) Coordinator UNIVERSITE DE NANTES Address Quai de tourville 1 44035 Nantes cedex 1 France See on map Region Pays de la Loire Pays de la Loire Loire-Atlantique Activity type Higher or Secondary Education Establishments Administrative Contact Clément Michaud (Mr.) Links Contact the organisation Opens in new window Website Opens in new window EU contribution No data
