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Engineering of new-generation protein secretion systems

Deliverables

Global models for protein secretion and secretion stress progression

Global models for protein secretion and secretion stress progression.

Supervisory board of the network established

Supervisory board of the network established for all partners

Model for protein trafficking through the secretory pathway

Model for protein trafficking through the secretory pathway.

List of common and critical conditions that are relevant during industrial fermentation processes

List of common and critical conditions that are relevant during industrial fermentation processes

New method for predicting membrane protein complexes

New method for predicting membrane protein complexes.

First quantitative data for selected membrane proteins

First quantitative data for selected membrane proteins

Membrane proteome data sets for B. subtilis + E. coli under all growth regimes

Membrane proteome data sets for B. subtilis + E. coli under all growth regimes.

Secretion bottlenecks identified

Secretion bottlenecks identified

Global quantitative analysis of membrane proteome

Global quantitative analysis of membrane proteome.

Definition of protein production stress responses in E. coli

Definition of protein production stress responses in E. coli.

Refined regulatory model including membrane proteins validated with proteomics data

Refined regulatory model including membrane proteins validated with proteomics data.

New algorithms that predict inter-helix pairings in membrane proteins

New algorithms that predict inter-helix pairings in membrane proteins.

Established model for secretion stress onset

Established model for secretion stress onset.

Validated membrane protein complexes and their dynamics

Validated membrane protein complexes and their dynamics.

Searching for OpenAIRE data...

Publications

Probing the quality control mechanism of the Escherichia coli twin-arginine translocase with folding variants of a de novo –designed heme protein

Author(s): George A. Sutherland, Katie J. Grayson, Nathan B. P. Adams, Daphne M. J. Mermans, Alexander S. Jones, Angus J. Robertson, Dirk B. Auman, Amanda A. Brindley, Fabio Sterpone, Pierre Tuffery, Philippe Derreumaux, P. Leslie Dutton, Colin Robinson, Andrew Hitchcock, C. Neil Hunter
Published in: Journal of Biological Chemistry, Issue 293/18, 2018, Page(s) 6672-6681, ISSN 0021-9258
DOI: 10.1074/jbc.ra117.000880

Far-reaching cellular consequences of tat deletion in Escherichia coli revealed by comprehensive proteome analyses

Author(s): Katarzyna M. Dolata, Isabel Guerrero Montero, Wayne Miller, Susanne Sievers, Thomas Sura, Christian Wolff, Rabea Schlüter, Katharina Riedel, Colin Robinson
Published in: Microbiological Research, Issue 218, 2019, Page(s) 97-107, ISSN 0944-5013
DOI: 10.1016/j.micres.2018.10.008

Comparative proteome analysis in an Escherichia coli CyDisCo strain identifies stress responses related to protein production, oxidative stress and accumulation of misfolded protein

Author(s): Isabel Guerrero Montero, Katarzyna Magdalena Dolata, Rabea Schlüter, Gilles Malherbe, Susanne Sievers, Daniela Zühlke, Thomas Sura, Emma Dave, Katharina Riedel, Colin Robinson
Published in: Microbial Cell Factories, Issue 18/1, 2019, ISSN 1475-2859
DOI: 10.1186/s12934-019-1071-7

The tunable pReX expression vector enables optimizing the T7-based production of membrane and secretory proteins in E. coli

Author(s): Grietje Kuipers, Alexandros Karyolaimos, Zhe Zhang, Nurzian Ismail, Gianluca Trinco, David Vikström, Dirk Jan Slotboom, Jan-Willem de Gier
Published in: Microbial Cell Factories, Issue 16/1, 2017, ISSN 1475-2859
DOI: 10.1186/s12934-017-0840-4

An ancient family of mobile genomic islands introducing cephalosporinase and carbapenemase genes in Enterobacteriaceae

Author(s): Suruchi Nepal, Florian Bonn, Stefano Grasso, Tim Stobernack, Anne de Jong, Kai Zhou, Ronald Wedema, Sigrid Rosema, Dörte Becher, Andreas Otto, John W. Rossen, Jan Maarten van Dijl, Erik Bathoorn
Published in: Virulence, Issue 9/1, 2018, Page(s) 1377-1389, ISSN 2150-5594
DOI: 10.1080/21505594.2018.1509666