Engineering of new-generation protein secretion systems

Risultati finali

Global models for protein secretion and secretion stress progression

Global models for protein secretion and secretion stress progression.

Supervisory board of the network established

Supervisory board of the network established for all partners

Model for protein trafficking through the secretory pathway

Model for protein trafficking through the secretory pathway.

List of common and critical conditions that are relevant during industrial fermentation processes
New method for predicting membrane protein complexes

New method for predicting membrane protein complexes.

First quantitative data for selected membrane proteins
Membrane proteome data sets for B. subtilis + E. coli under all growth regimes

Membrane proteome data sets for B. subtilis + E. coli under all growth regimes.

Secretion bottlenecks identified
Global quantitative analysis of membrane proteome

Global quantitative analysis of membrane proteome.

Definition of protein production stress responses in E. coli

Definition of protein production stress responses in E. coli.

Refined regulatory model including membrane proteins validated with proteomics data

Refined regulatory model including membrane proteins validated with proteomics data.

New algorithms that predict inter-helix pairings in membrane proteins

New algorithms that predict inter-helix pairings in membrane proteins.

Established model for secretion stress onset

Established model for secretion stress onset.

Validated membrane protein complexes and their dynamics

Validated membrane protein complexes and their dynamics.

Project website.

Project website

Pubblicazioni

Proteomic Charting of Imipenem Adaptive Responses in a Highly Carbapenem Resistant Clinical Enterobacter roggenkampii Isolate

Autori: Nepal S, Maaß S, Grasso S, Cavallo FM, Bartel J, Becher D, Bathoorn E, van Dijl JM
Pubblicato in: Antibiotics (Basel), Numero 10.5, 2021, Pagina/e 501, ISSN 2079-6382
Editore: MDPI
DOI: 10.3390/antibiotics10050501

Residue-by-residue analysis of cotranslational membrane protein integration in vivo

Autori: Nicolaus, F., Metola, A., Mermans, D., Liljenström, A., Krč, A., Abdullahi, S. M., Zimmer, M., Miller Iii, T. F., & von Heijne, G
Pubblicato in: eLife, Numero 10, 2021, Pagina/e 64302, ISSN 2050-084X
Editore: eLife Sciences Publications
DOI: 10.7554/elife.64302

Escherichia coli Can Adapt Its Protein Translocation Machinery for Enhanced Periplasmic Recombinant Protein Production

Autori: Alexandros Karyolaimos1†, Katarzyna Magdalena Dolata2†, Minia Antelo-Varela2†, Anna Mestre Borras1, Rageia Elfageih1, Susanne Sievers2, Dörte Becher2, Katharina Riedel2 and Jan-Willem de Gier
Pubblicato in: Front Bioeng Biotech, 2020, Pagina/e 465, ISSN 2296-4185
Editore: Frontiers Media S.A.
DOI: 10.3389/fbioe.2019.00465

Enhancing Recombinant Protein Yields in the E. coli Periplasm by Combining Signal Peptide and Production Rate Screening

Autori: Alexandros Karyolaimos1†, Henry Ampah-Korsah1†, Tamara Hillenaar1, Anna Mestre Borras1, Katarzyna Magdalena Dolata2, Susanne Sievers2, Katharina Riedel2, Robert Daniels1 and Jan-Willem de Gier1*
Pubblicato in: Front Bioeng Biotech, 2019, Pagina/e 1511, ISSN 1664-302X
Editore: Frontiers Media
DOI: 10.3389/fmicb.2019.01511

Ariadne's Thread in the Analytical Labyrinth of Membrane Proteins: Integration of Targeted and Shotgun Proteomics for Global Absolute Quantification of Membrane Proteins

Autori: Antelo-Varela M, Bartel J, Quesada-Ganuza A, Appel K, Bernal-Cabas M, Sura T, Otto A, Rasmussen M, van Dijl JM, Nielsen A, Maaß S, Becher D
Pubblicato in: Anal Chem, Numero 91(18), 2019, Pagina/e 11972-11980, ISSN 0003-2700
Editore: American Chemical Society
DOI: 10.1021/acs.analchem.9b02869

GP4: an integrated Gram-Positive Protein Prediction Pipeline for subcellular localization mimicking bacterial sorting

Autori: Gabarrini G, Grasso S, van Winkelhoff AJ, van Dijl JM
Pubblicato in: Brief Bioinformation, Numero 22(4), 2021, Pagina/e 302, ISSN 1477-4054
Editore: Oxford Academic
DOI: 10.1093/bib/bbaa302

Redirected Stress Responses in a Genome-Minimized 'midiBacillus' Strain with Enhanced Capacity for Protein Secretion

Autori: Aguilar Suárez R, Antelo-Varela M, Maaß S, Neef J, Becher D, van Dijl JM
Pubblicato in: mSystems, Numero 6(6), 2021, Pagina/e e0065521, ISSN 2379-5077
Editore: ASM Journals
DOI: 10.1128/msystems.00655-21

Signatures of cytoplasmic proteins in the exoproteome distinguish community- and hospital-associated methicillin-resistant Staphylococcus aureus USA300 lineages

Autori: Mekonnen SA, Palma Medina LM, Glasner C, Tsompanidou E, de Jong A, Grasso S, Schaffer M, Mäder U, Larsen AR, Gumpert H, Westh H, Völker U, Otto A, Becher D, van Dijl JM
Pubblicato in: Virulence, Numero 18(6), 2017, Pagina/e 891-907, ISSN 2150-5594
Editore: Landes Bioscience
DOI: 10.1080/21505594.2017.1325064

Strategies to Enhance Periplasmic Recombinant Protein Production Yields in Escherichia coli

Autori: Alexandros Karyolaimos and Jan-Willem de Gier
Pubblicato in: Front Bioeng Biotech, 2021, Pagina/e 797334, ISSN 2296-4185
Editore: Frontiers Media S.A.
DOI: 10.3389/fbioe.2021.797334

Functional association of the stress-responsive LiaH protein and the minimal TatAyCy protein translocase in Bacillus subtilis

Autori: Bernal-Cabas M, Miethke M, Antelo-Varela M, Aguilar Suárez R, Neef J, Schön L, Gabarrini G, Otto A, Becher D, Wolf D, van Dijl JM
Pubblicato in: Biochim Biophys Acta Mol Cell Res, Numero 1867(8), 2020, Pagina/e 19, ISSN 2079-6382
Editore: Elsevier
DOI: 10.1016/j.bbamcr.2020.118719

Probing the quality control mechanism of the Escherichia coli twin-arginine translocase with folding variants of a de novo –designed heme protein

Autori: George A. Sutherland, Katie J. Grayson, Nathan B. P. Adams, Daphne M. J. Mermans, Alexander S. Jones, Angus J. Robertson, Dirk B. Auman, Amanda A. Brindley, Fabio Sterpone, Pierre Tuffery, Philippe Derreumaux, P. Leslie Dutton, Colin Robinson, Andrew Hitchcock, C. Neil Hunter
Pubblicato in: Journal of Biological Chemistry, Numero 293/18, 2018, Pagina/e 6672-6681, ISSN 0021-9258
Editore: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.ra117.000880

Far-reaching cellular consequences of tat deletion in Escherichia coli revealed by comprehensive proteome analyses

Autori: Katarzyna M. Dolata, Isabel Guerrero Montero, Wayne Miller, Susanne Sievers, Thomas Sura, Christian Wolff, Rabea Schlüter, Katharina Riedel, Colin Robinson
Pubblicato in: Microbiological Research, Numero 218, 2019, Pagina/e 97-107, ISSN 0944-5013
Editore: Elsevier BV
DOI: 10.1016/j.micres.2018.10.008

Comparative proteome analysis in an Escherichia coli CyDisCo strain identifies stress responses related to protein production, oxidative stress and accumulation of misfolded protein

Autori: Isabel Guerrero Montero, Katarzyna Magdalena Dolata, Rabea Schlüter, Gilles Malherbe, Susanne Sievers, Daniela Zühlke, Thomas Sura, Emma Dave, Katharina Riedel, Colin Robinson
Pubblicato in: Microbial Cell Factories, Numero 18/1, 2019, ISSN 1475-2859
Editore: BioMed Central
DOI: 10.1186/s12934-019-1071-7

The tunable pReX expression vector enables optimizing the T7-based production of membrane and secretory proteins in E. coli

Autori: Grietje Kuipers, Alexandros Karyolaimos, Zhe Zhang, Nurzian Ismail, Gianluca Trinco, David Vikström, Dirk Jan Slotboom, Jan-Willem de Gier
Pubblicato in: Microbial Cell Factories, Numero 16/1, 2017, ISSN 1475-2859
Editore: BioMed Central
DOI: 10.1186/s12934-017-0840-4

An ancient family of mobile genomic islands introducing cephalosporinase and carbapenemase genes in Enterobacteriaceae

Autori: Suruchi Nepal, Florian Bonn, Stefano Grasso, Tim Stobernack, Anne de Jong, Kai Zhou, Ronald Wedema, Sigrid Rosema, Dörte Becher, Andreas Otto, John W. Rossen, Jan Maarten van Dijl, Erik Bathoorn
Pubblicato in: Virulence, Numero 9/1, 2018, Pagina/e 1377-1389, ISSN 2150-5594
Editore: Landes Bioscience
DOI: 10.1080/21505594.2018.1509666

SppI Forms a Membrane Protein Complex with SppA and Inhibits Its Protease Activity in Bacillus subtilis

Autori: Henriques G, McGovern S, Neef J, Antelo-Varela M, Götz F, Otto A, Becher D, van Dijl JM, Jules M, Delumeau O
Pubblicato in: mSphere, Numero 5(5), 2020, Pagina/e 00724-20, ISSN 2379-5042
Editore: ASM Journals
DOI: 10.1128/msphere.00724-20

Upstream charged and hydrophobic residues impact the timing of membrane insertion of transmembrane helices

Autori: Nicolaus, F., Ibrahimi, F., den Besten, A., & von Heijne, G
Pubblicato in: FEBS letters, Numero 10.1002, 2022, ISSN 0014-5793
Editore: Elsevier BV
DOI: 10.1002/1873-3468.14286

Double trouble: Bacillus depends on a functional Tat machinery to avoid severe oxidative stress and starvation upon entry into a NaCl-depleted environment

Autori: Prajapati B, Bernal-Cabas M, López-Álvarez M, Schaffer M, Bartel J, Rath H, Steil L, Becher D, Völker U, Mäder U, van Dijl JM
Pubblicato in: Biochim Biophys Acta Mol Cell Res, Numero 1868(2), 2020, Pagina/e 118914, ISSN 0167-4889
Editore: Elsevier BV
DOI: 10.1016/j.bbamcr.2020.118914

"Membrane Modulation of Super-Secreting ""midiBacillus"" Expressing the Major Staphylococcus aureus Antigen - A Mass-Spectrometry-Based Absolute Quantification Approach"

Autori: Antelo-Varela M, Aguilar Suárez R, Bartel J, Bernal-Cabas M, Stobernack T, Sura T, van Dijl JM, Maaß S, Becher D
Pubblicato in: Front Bioeng Biotechnol, Numero 8, 2020, Pagina/e 143, ISSN 2296-4185
Editore: Frontiers Media S.A
DOI: 10.3389/fbioe.2020.00143

Risultati finali

Pubblicazioni

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