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Development of Selective Carbohydrate Immunomodulators Targeting C-type Lectin Receptors on Antigen Presenting Cells

Deliverables

Glycan antigen library

Library of β-glucans, α-mannans rhamnans, fucoidans polyN-acetylglucosamines (PNAG) and lipomannans synthesized by automated solid-phase synthesis

Murine and human CLRs

Recombinant expression of murine and human CLRs, expression cloning of new CLRs

Glycodendrimers

(Fluorescent) glycan functionalized dendritic scaffolds prepared

Neoglycoproteins

BSA and OVA-glycoconjugates prepared, GFP-neoglycoproteins expressed

Cell surface binding

Classification of cell surface lectins by their in vitro binding capacity

Glycan binding of CLRs

Determine the glycan binding specificities of recombinant CLRs expressed in WP1

Website online

Design and develop network website, regular updates on network progress and events

Searching for OpenAIRE data...

Publications

Facile access to pseudo-thio - 1,2 - dimannoside, a new glycomimetic DC-SIGN antagonist


Published in: ISSN 0968-0896
DOI: 10.1016/j.bmc.2017.03.046

Specific anti-glycan antibodies are sustained during and after parasite clearance in Schistosoma japonicum-infected rhesus macaques

Author(s): Y. Y. Michelle Yang, Xiao Hong Li, Katarzyna Brzezicka, Niels-Christian Reichardt, R. Alan Wilson, Angela van Diepen, Cornelis H. Hokke
Published in: PLOS Neglected Tropical Diseases, Issue 11/2, 2017, Page(s) e0005339, ISSN 1935-2735
DOI: 10.1371/journal.pntd.0005339

C-Type Lectin Receptor (CLR)–Fc Fusion Proteins As Tools to Screen for Novel CLR/Bacteria Interactions: An Exemplary Study on Preselected Campylobacter jejuni Isolates

Author(s): Sabine Mayer, Rebecca Moeller, João T. Monteiro, Kerstin Ellrott, Christine Josenhans, Bernd Lepenies
Published in: Frontiers in Immunology, Issue 9, 2018, ISSN 1664-3224
DOI: 10.3389/fimmu.2018.00213

Rapid and efficient synthesis of α(1–2)mannobiosides

Author(s): José J. Reina, Antonio Di Maio, Javier Ramos-Soriano, Rute C. Figueiredo, Javier Rojo
Published in: Org. Biomol. Chem., Issue 14/10, 2016, Page(s) 2873-2882, ISSN 1477-0520
DOI: 10.1039/C6OB00083E

Chemoenzymatic Synthesis of N-glycan Positional Isomers and Evidence for Branch Selective Binding by Monoclonal Antibodies and Human C-type Lectin Receptors

Author(s): Begoña Echeverria, Sonia Serna, Silvia Achilli, Corinne Vivès, Julie Pham, Michel Thépaut, Cornelis H. Hokke, Franck Fieschi, Niels-Christian Reichardt
Published in: ACS Chemical Biology, 2018, ISSN 1554-8929
DOI: 10.1021/acschembio.8b00431

ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences

Author(s): Chethan D. Shanthamurthy, Prashant Jain, Sharon Yehuda, João T. Monteiro, Shani Leviatan Ben-Arye, Balamurugan Subramani, Bernd Lepenies, Vered Padler-Karavani, Raghavendra Kikkeri
Published in: Scientific Reports, Issue 8/1, 2018, ISSN 2045-2322
DOI: 10.1038/s41598-018-24333-y

Fucosylated Antigens in Cancer: An Alliance toward Tumor Progression, Metastasis, and Resistance to Chemotherapy

Author(s): Athanasios Blanas, Neha M. Sahasrabudhe, Ernesto Rodríguez, Yvette van Kooyk, Sandra J. van Vliet
Published in: Frontiers in Oncology, Issue 8, 2018, ISSN 2234-943X
DOI: 10.3389/fonc.2018.00039

Automated glycan assembly as an enabling technology

Author(s): Alonso Pardo-Vargas, Martina Delbianco, Peter H Seeberger
Published in: Current Opinion in Chemical Biology, Issue 46, 2018, Page(s) 48-55, ISSN 1367-5931
DOI: 10.1016/j.cbpa.2018.04.007

C-type lectin receptor DCIR modulates immunity to tuberculosis by sustaining type I interferon signaling in dendritic cells

Author(s): Anthony Troegeler, Ingrid Mercier, Céline Cougoule, Danilo Pietretti, André Colom, Carine Duval, Thien-Phong Vu Manh, Florence Capilla, Renaud Poincloux, Karine Pingris, Jérôme Nigou, Jörg Rademann, Marc Dalod, Frank A. W. Verreck, Talal Al Saati, Geanncarlo Lugo-Villarino, Bernd Lepenies, Denis Hudrisier, Olivier Neyrolles
Published in: Proceedings of the National Academy of Sciences, Issue 114/4, 2017, Page(s) E540-E549, ISSN 0027-8424
DOI: 10.1073/pnas.1613254114

CD169+ Macrophages Capture and Dendritic Cells Instruct: The Interplay of the Gatekeeper and the General of the Immune System

Author(s): Joanna Grabowska, Miguel A. Lopez-Venegas, Alsya J. Affandi, Joke M. M. den Haan
Published in: Frontiers in Immunology, Issue 9, 2018, ISSN 1664-3224
DOI: 10.3389/fimmu.2018.02472

Modulation of Immune Tolerance via Siglec-Sialic Acid Interactions

Author(s): Joyce Lübbers, Ernesto Rodríguez, Yvette van Kooyk
Published in: Frontiers in Immunology, Issue 9, 2018, ISSN 1664-3224
DOI: 10.3389/fimmu.2018.02807

Glycopeptides as Targets for Dendritic Cells: Exploring MUC1 Glycopeptides Binding Profile toward Macrophage Galactose-Type Lectin (MGL) Orthologs

Author(s): Gerard Artigas, João T. Monteiro, Hiroshi Hinou, Shin-Ichiro Nishimura, Bernd Lepenies, Fayna Garcia-Martin
Published in: Journal of Medicinal Chemistry, Issue 60/21, 2017, Page(s) 9012-9021, ISSN 0022-2623
DOI: 10.1021/acs.jmedchem.7b01242

Pseudo-Mannosylated DC-SIGN Ligands as Immunomodulants

Author(s): Angela Berzi, Stefania Ordanini, Ben Joosten, Daria Trabattoni, Alessandra Cambi, Anna Bernardi, Mario Clerici
Published in: Scientific Reports, Issue 6, 2016, Page(s) 35373, ISSN 2045-2322
DOI: 10.1038/srep35373

Opportunities for glyconanomaterials in personalized medicine

Author(s): Niels-Christian Reichardt, Manuel Martín-Lomas, Soledad Penadés
Published in: Chem. Commun., Issue 52/92, 2016, Page(s) 13430-13439, ISSN 1359-7345
DOI: 10.1039/C6CC04445J

Microbe-focused glycan array screening platform

Author(s): Andreas Geissner, Anika Reinhardt, Christoph Rademacher, Timo Johannssen, João Monteiro, Bernd Lepenies, Michel Thépaut, Franck Fieschi, Jana Mrázková, Michaela Wimmerova, Frank Schuhmacher, Sebastian Götze, Dan Grünstein, Xiaoqiang Guo, Heung Sik Hahm, Jeyakumar Kandasamy, Daniele Leonori, Christopher E. Martin, Sharavathi G. Parameswarappa, Sandip Pasari, Mark K. Schlegel, Hidenori Tanaka,
Published in: Proceedings of the National Academy of Sciences, Issue 116/6, 2019, Page(s) 1958-1967, ISSN 0027-8424
DOI: 10.1073/pnas.1800853116

Influence of Core β-1,2-Xylosylation on Glycoprotein Recognition by Murine C-type Lectin Receptors and Its Impact on Dendritic Cell Targeting

Author(s): Katarzyna Brzezicka, Uwe Vogel, Sonia Serna, Timo Johannssen, Bernd Lepenies, Niels-Christian Reichardt
Published in: ACS Chemical Biology, Issue 11/8, 2016, Page(s) 2347-2356, ISSN 1554-8929
DOI: 10.1021/acschembio.6b00265

Myeloid C-Type Lectin Receptors in Viral Recognition and Antiviral Immunity

Author(s): João Monteiro, Bernd Lepenies
Published in: Viruses, Issue 9/3, 2017, Page(s) 59, ISSN 1999-4915
DOI: 10.3390/v9030059

NMR and Molecular Recognition of N-Glycans: Remote Modifications of the Saccharide Chain Modulate Binding Features

Author(s): Ana Gimeno, Niels-Christian Reichardt, F. Javier Cañada, Lukas Perkams, Carlo Unverzagt, Jesús Jiménez-Barbero, Ana Ardá
Published in: ACS Chemical Biology, Issue 12/4, 2017, Page(s) 1104-1112, ISSN 1554-8929
DOI: 10.1021/acschembio.6b01116

Multivalent Lectin-Glycan Interactions in the Immune System

Author(s): Joao T. Monteiro and Bernd Lepenies
Published in: Multivalency: Concepts, Research and Applications, 2017, Page(s) 325-344