Risultati finali Documents, reports (45) Methods for preprocessing and processing of spectral data Presentation of a document describing methods for preprocessing and processing of spectral data. Proof of concept to determine GAGs composition in cancer stromal tissue Report on the experimental results on the proof of concept to determine GAGs composition in cancer stromal tissue. Changes in invasive behaviour Report on experimental results on changes in invasive behaviour of cancer cells subjected to PG/GAG manipulation. Functional in vitro studies Report on the experimental results of functional in vitro experiments after treatments of cancer cell lines stimulating or inhibiting HBP, O-GlcNAcylation and epigenetic modifications. Analysis of angiogenic factors in immunomodulatory cells Report on the experimental results of the analysis of angiogenic factors in immunomodulatory cells in the context of inflammatory breast cancer. microRNA regulation of Glypican-1, Syndecan-2 and HAS1-3 in breast and colon cancer Report on experimental results of microRNA regulation of Glypican-1, Syndecan-2 and HAS1-3 in breast and colon cancer. Characterization of HS disaccharide structure from 8 breast cancer cell lines with altered Syndecan-1, 3-O-HS sulfation and 2-O-HS sulfation Report on experimental results regarding the characterization of HS disaccharide structure from 8 breast cancer cell lines with altered Syndecan-1, 3-O-HS sulfation and 2-O-HS sulfation. Correlation of GAG structure with clinicopathological parameters Report on the correlation of GAG structure with clinicopathological parameters, including statistical analysis. microRNA-regulation of Syndecan-1 in clinical IBC specimens Report on experimental results of microRNA-regulation of Syndecan-1 in clinical IBC specimens. Flow cytometric analysis of SP and ALDH phenotype Report on the experimental results of flow cytometric analysis of SP and ALDH phenotype of cancer stem cells. Chemotherapeutic resistance Report on experimental results regarding chemotherapeutic resistance of PG/GAG-manipulated cancer stem cells. In vivo study on the premetastatic niche Report on the experimental results of the in vivo study on the premetastatic niche. Diagnostic value of Syndecan-1 in IBC Report on the diagnostic value of Syndecan-1 in IBC including statistical analysis. ChIP experiments Report on the experimental results of chromatin immunoprecipitation experiments. Nanostic analysis Report on the experimental results of the nanostic analysis of cancer cells. Standardized protocol for FTIR and RAMAN spectroscopic analysis of GAGs in cells and conditioned media. Presentation of a standardized experimental protocol for FTIR and RAMAN spectroscopic analysis of GAGs in cells and conditioned media. qPCR analysis of angiogenic factors in tumors Experimental data report on qPCR analysis of angiogenic factors in tumors related to syndecan-1. Spectroscopic delineation of the expression of GAGs in mice lung metastatic nodules Report on the experimental results of spectroscopic analysis of the expression of GAGs in mice lung metastatic nodules. 3D culture Report on the experimental results of 3D cultures of cancer stem cell-enriched cultures. Functional impact of miRNA-dependent PG and HAS regulation Report on the experimental results of the functional impact of miRNA-dependent PG and HAS regulation. Spectroscopic delineation of the expression of GAGs in murine melanoma, and PG-manuipulated cancer cell lines Report on the experimental results of the spectroscopic analysis of the expression of GAGs in murine melanoma, and PG-manuipulated cancer cell lines. Mechanistic in vitro investigation of Syndecan-dependenttranscriptional activation Report on the experimental results of the mechanistic in vitro investigation of Syndecan-dependent transcriptional activation. Characterization of HA molecular weight from tumor and control tissue of at least three in vivo mouse tumor models Report on the experimental results of the characterization of HA molecular weight from tumor and control tissue of at least three in vivo mouse tumor models. In vitro analysis of microRNA-dependent Syndecan-1 regulation in IBC Report on experimental results of the in vitro analysis of microRNA-dependent Syndecan-1 regulation in IBC. Standardized Protocol for Tissue Microarray Analysis by FTIR and RAMAN spectroscopy Presentation of a standardized Protocol (Document) for Tissue Microarray Analysis by FTIR and RAMAN spectroscopy. Analysis of signaling pathways Report on experimental results of signal transduction analysis in cancer stem cells. Immune cells in the microenvironment Experimental data report on the analysis of immune cells in the tumor microenvironment. Role of Syndecan-1 oligomerization in FAK activation Report on the experimental results on the role of Syndecan-1 oligomerization in FAK activation. Therapeutic potential of Ascidian GAGs Report on experimental data regarding the therapeutic potential of Ascidian GAGs in malignant disease. Impact of PG/HA on DNA damage response Report on the experimental results of the impact of PG/HA on the DNA damage response. Proof of concept for in vivo Raman probes for diagnosis of melanoma in patients and application to tumor-associated macrophages Report on the experimental results delivering proof of concept for in vivo Raman probes for diagnosis of melanoma in patients and application to tumor-associated macrophages. Angiogenesis regulators Experimental data report on the analysis of hyaluronic acid-dependent angiogenesis regulators. Impact of conventional therapeutics on the premetastatic niche Report on experimental data of the impact of conventional therapeutics on the premetastatic niche. In vivo model of hepatocarcinogenesis Exytablishment of, and report on the experimental results of the in vivo model of hepatocarcinogenesis. Inhibitory effect of 4- MU in vivo Experimental data report on the inhibitory effect of 4- MU in vivo. Evaluation of HA related enzyme expression Experimental data report on the evaluation of Evaluation of HA related enzyme expression in mouse cancerous tissues. Microvascular density Experimental data report on the analysis of microvascular densitty in in vivo models. Impact of PGs and GAGs on premature senescence of cancer cells Report on experimental results of the studies on the impact of PGs and GAGs on premature senescence of cancer cells. Epigenetic HAS2 promoter regulation Report on the experimental results of epigenetic HAS2 promoter regulation. Flow cytometric and qPCR analysis of stem cell markers Report on experimental results of flow cytometric and qPCR analysis of stem cell markers. In vitro functional assays in a novel 3D culture system Establishment and report on experimental data of functional in vitro assays in a novel 3D culture system. In vitro study of the premetastatic niche Report on experimental results on the in vitro study of the premetastatic niche. Changes in EMT marker expression Report on the experimental results of changes in EMT marker expression in cancer cells subjected to PG/GAG manipulation. Analysis of glioblastoma cell line GAG structure Report on the experimental results of the analysis of glioblastoma cell line GAG structure. Role of PGs in TGFbeta induced EMT Report of experimental results on the role of PGs in TGFbeta induced EMT. Websites, patent fillings, videos etc. (3) Workshop on PGs and GAGs in metastasis Website reporting and dissemination on the Workshop on PGs and GAGs in metastasis organized by WWU. Workshop on epigenetic regulation Website report and dissemination on the workshop epigenetic regulation of PG and GAG expression and function organized by partner UNINSUBRIA. Establichment of GLYCANC Website Establishment of GLYCANC website and social media presence (Facebook, Research Gate). Pubblicazioni Peer reviewed articles (30) Two ways of epigenetic silencing of TFPI2 in cervical cancer Autori: Alexandra Fullár, Katalin Karászi, Péter Hollósi, Gábor Lendvai, Lászlóné Oláh, Andrea Reszegi, Zoltán Papp, Gábor Sobel, József Dudás, Ilona Kovalszky Pubblicato in: PLOS ONE, Issue 15/6, 2020, Page(s) e0234873, ISSN 1932-6203 Editore: Public Library of Science DOI: 10.1371/journal.pone.0234873 Label-Free Infrared Spectral Histology of Skin Tissue Part II: Impact of a Lumican-Derived Peptide on Melanoma Growth Autori: Stéphane Brézillon, Valérie Untereiner, Hossam Taha Mohamed, Estelle Ahallal, Isabelle Proult, Pierre Nizet, Camille Boulagnon-Rombi, Ganesh. D. Sockalingum Pubblicato in: Frontiers in Cell and Developmental Biology, Issue 8, 2020, ISSN 2296-634X Editore: Frontiers Media SA DOI: 10.3389/fcell.2020.00377 Induction of heparanase via IL-10 correlates with a high infiltration of CD163+ M2-type tumor-associated macrophages in inflammatory breast carcinomas Autori: Mennatullah El-Nadi, Hebatallah Hassan, Moshira Ezzat Saleh, Eyyad Nassar, Yahia Mahmoud Ismail, Mahmoud Amer, Burkhard Greve, Martin Götte, Mohamed El-Shinawi, Sherif Abdelaziz Ibrahim Pubblicato in: Matrix Biology Plus, Issue 6-7, 2020, Page(s) 100030, ISSN 2590-0285 Editore: Elsevier DOI: 10.1016/j.mbplus.2020.100030 The heparan sulfate proteoglycan syndecan‐1 regulates colon cancer stem cell function via a focal adhesion kinase—Wnt signaling axis Autori: Sampath Kumar Katakam, Valeria Tria, Wey‐Cheng Sim, George W. Yip, Stefano Molgora, Theodoros Karnavas, Eslam A. Elghonaimy, Paride Pelucchi, Eleonora Piscitelli, Sherif Abdelaziz Ibrahim, Ileana Zucchi, Rolland Reinbold, Burkhard Greve, Martin Götte Pubblicato in: The FEBS Journal, Issue 288/2, 2021, Page(s) 486-506, ISSN 1742-464X Editore: Blackwell Publishing Inc. DOI: 10.1111/febs.15356 Infrared Microspectroscopy and Imaging Analysis of Inflammatory and Non-Inflammatory Breast Cancer Cells and Their GAG Secretome Autori: Hossam Taha Mohamed, Valérie Untereiner, Gianfelice Cinque, Sherif Abdelaziz Ibrahim, Martin Götte, Nguyet Que Nguyen, Romain Rivet, Ganesh D. Sockalingum, Stéphane Brézillon Pubblicato in: Molecules, Issue 25/18, 2020, Page(s) 4300, ISSN 1420-3049 Editore: Multidisciplinary Digital Publishing Institute (MDPI) DOI: 10.3390/molecules25184300 Insights into the key roles of epigenetics in matrix macromolecules-associated wound healing Autori: Zoi Piperigkou, Martin Götte, Achilleas D. Theocharis, Nikos K. Karamanos Pubblicato in: Advanced Drug Delivery Reviews, 2017, ISSN 0169-409X Editore: Elsevier BV DOI: 10.1016/j.addr.2017.10.008 Extracellular matrix alterations in senescent cells and their significance in tissue homeostasis Autori: Eleni Mavrogonatou, Harris Pratsinis, Adamantia Papadopoulou, Nikos K. Karamanos, Dimitris Kletsas Pubblicato in: Matrix Biology, 2017, ISSN 0945-053X Editore: Elsevier BV DOI: 10.1016/j.matbio.2017.10.004 Changes in hyaluronan deposition in the rat myenteric plexus after experimentally-induced colitis Autori: Viviana Filpa, Michela Bistoletti, Ilaria Caon, Elisabetta Moro, Annalisa Grimaldi, Paola Moretto, Andreina Baj, Maria Cecilia Giron, Evgenia Karousou, Manuela Viola, Francesca Crema, Gianmario Frigo, Alberto Passi, Cristina Giaroni, Davide Vigetti Pubblicato in: Scientific Reports, Issue 7/1, 2017, ISSN 2045-2322 Editore: Nature Publishing Group DOI: 10.1038/s41598-017-18020-7 Syndecan-1 inhibits early stages of liver fibrogenesis by interfering with TGFβ1 action and upregulating MMP14 Autori: Eszter Regős, Hadeer Hesham Abdelfattah, Andrea Reszegi, László Szilák, Klára Werling, Gábor Szabó, András Kiss, Zsuzsa Schaff, Ilona Kovalszky, Kornélia Baghy Pubblicato in: Matrix Biology, 2018, ISSN 0945-053X Editore: Elsevier BV DOI: 10.1016/j.matbio.2018.02.008 Shed proteoglycans in tumor stroma Autori: Zoi Piperigkou, Benedikt Mohr, Nikos Karamanos, Martin Götte Pubblicato in: Cell and Tissue Research, 2016, ISSN 0302-766X Editore: Springer Verlag DOI: 10.1007/s00441-016-2452-4 Extracellular matrix functions in lung cancer Autori: Martin Götte, Ilona Kovalszky Pubblicato in: Matrix Biology, 2018, ISSN 0945-053X Editore: Elsevier BV DOI: 10.1016/j.matbio.2018.02.018 Tumor-suppressive functions of 4-MU on breast cancer cells of different ER status: Regulation of hyaluronan/HAS2/CD44 and specific matrix effectors Autori: Theodoros T. Karalis, Paraskevi Heldin, Demitrios H. Vynios, Thomas Neill, Simone Buraschi, Renato V. Iozzo, Nikos K. Karamanos, Spyros S. Skandalis Pubblicato in: Matrix Biology, 2018, ISSN 0945-053X Editore: Elsevier BV DOI: 10.1016/j.matbio.2018.04.007 Co-treatment of tumor cells with hyaluronan plus doxorubicin affects endothelial cell behavior independently of VEGF expression Autori: Daiana L. Vitale, Fiorella M. Spinelli, Daiana Del Dago, Antonella Icardi, Gianina Demarchi, Ilaria Caon, Mariana García, Marcela F. Bolontrade, Alberto Passi, Carolina Cristina, Laura Alaniz Pubblicato in: Oncotarget, Issue 9/93, 2018, ISSN 1949-2553 Editore: Impact Journals DOI: 10.18632/oncotarget.26379 microRNA miR-142-3p Inhibits Breast Cancer Cell Invasiveness by Synchronous Targeting of WASL, Integrin Alpha V, and Additional Cytoskeletal Elements Autori: Alexander Schwickert, Esther Weghake, Kathrin Brüggemann, Annika Engbers, Benjamin F. Brinkmann, Björn Kemper, Jochen Seggewiß, Christian Stock, Klaus Ebnet, Ludwig Kiesel, Christoph Riethmüller, Martin Götte Pubblicato in: PLOS ONE, Issue 10/12, 2015, Page(s) e0143993, ISSN 1932-6203 Editore: Public Library of Science DOI: 10.1371/journal.pone.0143993 Syndecan-1 in Liver Diseases Autori: Eszter Regős, Katalin Karászi, Andrea Reszegi, András Kiss, Zsuzsa Schaff, Kornélia Baghy, Ilona Kovalszky Pubblicato in: Pathology & Oncology Research, 2019, ISSN 1219-4956 Editore: Springer Verlag DOI: 10.1007/s12253-019-00617-0 IGF-IR cooperates with ERα to inhibit breast cancer cell aggressiveness by regulating the expression and localisation of ECM molecules Autori: Nikolaos A. Afratis, Panagiotis Bouris, Spyros S. Skandalis, Hinke A. Multhaupt, John R. Couchman, Achilleas D. Theocharis, Nikos K. Karamanos Pubblicato in: Scientific Reports, Issue 7, 2017, Page(s) 40138, ISSN 2045-2322 Editore: Nature Publishing Group DOI: 10.1038/srep40138 Implementation of infrared and Raman modalities for glycosaminoglycan characterization in complex systems Autori: Hossam Taha Mohamed, Valérie Untereiner, Ganesh D. Sockalingum, Stéphane Brézillon Pubblicato in: Glycoconjugate Journal, 2016, ISSN 0282-0080 Editore: Kluwer Academic Publishers DOI: 10.1007/s10719-016-9743-6 Hyaluronan preconditioning of monocytes/macrophages affects their angiogenic behavior and regulation of TSG ‐6 expression in a tumor type‐specific manner Autori: Fiorella M. Spinelli, Daiana L. Vitale, Antonella Icardi, Ilaria Caon, Alejandra Brandone, Paula Giannoni, Virginia Saturno, Alberto Passi, Mariana García, Ina Sevic, Laura Alaniz Pubblicato in: The FEBS Journal, 2019, ISSN 1742-464X Editore: Blackwell Publishing Inc. DOI: 10.1111/febs.14871 Proteoglycans and glycosaminoglycans as regulators of cancer stem cell function and therapeutic resistance Autori: Daiana Vitale, Sampath Kumar Katakam, Burkhard Greve, Bohee Jang, Eok-Soo Oh, Laura Alaniz, Martin Götte Pubblicato in: The FEBS Journal, 2019, ISSN 1742-464X Editore: Blackwell Publishing Inc. DOI: 10.1111/febs.14967 Tumor-specific inhibitory action of decorin on different hepatoma cell lines Autori: Zsolt Horváth, Andrea Reszegi, László Szilák, Titanilla Dankó, Ilona Kovalszky, Kornélia Baghy Pubblicato in: Cellular Signalling, 2019, Page(s) 109354, ISSN 0898-6568 Editore: Elsevier BV DOI: 10.1016/j.cellsig.2019.109354 The immunomodulatory role of tumor Syndecan-1 (CD138) on ex vivo tumor microenvironmental CD4+ T cell polarization in inflammatory and non-inflammatory breast cancer patients Autori: Moshira Ezzat Saleh, Ramy Gadalla, Hebatallah Hassan, Ahmed Afifi, Martin Götte, Mohamed El-Shinawi, Mona Mostafa Mohamed, Sherif Abdelaziz Ibrahim Pubblicato in: PLOS ONE, Issue 14/5, 2019, Page(s) e0217550, ISSN 1932-6203 Editore: Public Library of Science DOI: 10.1371/journal.pone.0217550 Molecular size-dependent specificity of hyaluronan on functional properties, morphology and matrix composition of mammary cancer cells Autori: Anastasia-Gerasimoula Tavianatou, Zoi Piperigkou, Carlo Barbera, Riccardo Beninatto, Valentina Masola, Ilaria Caon, Maurizio Onisto, Marco Franchi, Devis Galesso, Nikos K. Karamanos Pubblicato in: Matrix Biology Plus, 2019, Page(s) 100008, ISSN 2590-0285 Editore: Elsevier DOI: 10.1016/j.mbplus.2019.100008 Characterization of inflammatory breast cancer: a vibrational microspectroscopy and imaging approach at the cellular and tissue level Autori: Hossam Taha Mohamed, Valérie Untereiner, Isabelle Proult, Sherif Abdelaziz Ibrahim, Martin Götte, Mohamed El-Shinawi, Mona Mostafa Mohamed, Ganesh D. Sockalingum, Stéphane Brézillon Pubblicato in: The Analyst, Issue 143/24, 2018, Page(s) 6103-6112, ISSN 0003-2654 Editore: Royal Society of Chemistry DOI: 10.1039/c8an01292j Dissecting the role of hyaluronan synthases in the tumor microenvironment Autori: Alberto Passi, Davide Vigetti, Simone Buraschi, Renato V. Iozzo Pubblicato in: The FEBS Journal, Issue 286/15, 2019, Page(s) 2937-2949, ISSN 1742-464X Editore: Blackwell Publishing Inc. DOI: 10.1111/febs.14847 The extracellular matrix as a multitasking player in disease Autori: Achilleas D. Theocharis, Dimitra Manou, Nikos K. Karamanos Pubblicato in: The FEBS Journal, Issue 286/15, 2019, Page(s) 2830-2869, ISSN 1742-464X Editore: Blackwell Publishing Inc. DOI: 10.1111/febs.14818 Probing glycosaminoglycan spectral signatures in live cells and their conditioned media by Raman microspectroscopy Autori: S. Brézillon, V. Untereiner, H. T. Mohamed, J. Hodin, A. Chatron-Colliet, F.-X. Maquart, G. D. Sockalingum Pubblicato in: The Analyst, Issue 142/8, 2017, Page(s) 1333-1341, ISSN 0003-2654 Editore: Royal Society of Chemistry DOI: 10.1039/C6AN01951J Roles and targeting of the HAS/hyaluronan/CD44 molecular system in cancer Autori: Evgenia Karousou, Suniti Misra, Shibnath Ghatak, Katalin Dobra, Martin Götte, Davide Vigetti, Alberto Passi, Nikos K. Karamanos, Spyros S. Skandalis Pubblicato in: Matrix Biology, Issue 59, 2017, Page(s) 3-22, ISSN 0945-053X Editore: Elsevier BV DOI: 10.1016/j.matbio.2016.10.001 Syndecan-1 is a novel molecular marker for triple negative inflammatory breast cancer and modulates the cancer stem cell phenotype via the IL-6/STAT3, Notch and EGFR signaling pathways Autori: Sherif Abdelaziz Ibrahim, Ramy Gadalla, Eslam A. El-Ghonaimy, Omnia Samir, Hossam Taha Mohamed, Hebatallah Hassan, Burkhard Greve, Mohamed El-Shinawi, Mona Mostafa Mohamed, Martin Götte Pubblicato in: Molecular Cancer, Issue 16/1, 2017, Page(s) 57, ISSN 1476-4598 Editore: BioMed Central DOI: 10.1186/s12943-017-0621-z Estrogen receptor beta as epigenetic mediator of miR-10b and miR-145 in mammary cancer Autori: Zoi Piperigkou, Marco Franchi, Martin Götte, Nikos K. Karamanos Pubblicato in: Matrix Biology, 2017, ISSN 0945-053X Editore: Elsevier BV DOI: 10.1016/j.matbio.2017.08.002 Inhibition of Heparanase in Pediatric Brain Tumor Cells Attenuates their Proliferation, Invasive Capacity, and In Vivo Tumor Growth Autori: Argyris Spyrou, Soumi Kundu, Lulu Haseeb, Di Yu, Tommie Olofsson, Keith Dredge, Edward Hammond, Uri Barash, Israel Vlodavsky, Karin Forsberg-Nilsson Pubblicato in: Molecular Cancer Therapeutics, Issue 16/8, 2017, Page(s) 1705-1716, ISSN 1535-7163 Editore: American Association for Cancer Research DOI: 10.1158/1535-7163.MCT-16-0900 È in corso la ricerca di dati su OpenAIRE... Si è verificato un errore durante la ricerca dei dati su OpenAIRE Nessun risultato disponibile