Objetivo Translocation of ions and molecules is ubiquitous in biology and technology. Despite the tremendous amount of technical development, biological systems are still much more sophisticated in exerting exquisite control over active and passive translocation through nanopores in membranes than their existing synthetic mimics. This proposal aims to build novel designer nanopores that can match naturally evolved systems. For this we have to control all three stages of translocation: 1) diffusion and entry into, 2) diffusion in, and 3) exit from the nanopore. To gain fundamental insight into the translocation process we will employ microfluidic channels combined with holographic optical tweezers. Results from the microscale model system will be directly translated to nanoscale pores built with DNA origami nanotechnology. Our microfluidic experiments will automatically track diffusing spherical and non-spherical particles in artificial channels. Facilitated membrane transport will be mimicked by holographic optical tweezers providing full control over the translocation process. We will clarify how translocation depends on particle-particle, particle-channel, and particle-channel-entrance interactions. The generic principles discovered on the microscale will guide the design of artificial nanopores made by DNA origami self-assembly. Our DNA origami based designer nanopores will lead to a novel class of transporters for molecules, ions, and water through solid-state and lipid membranes. The project will generate a quantitative understanding of membrane transport processes, test existing theoretical models with unprecedented experimental control, and introduce a novel approach to design active and passive nanopores built from DNA. Ámbito científico natural sciencesphysical sciencesclassical mechanicsfluid mechanicsmicrofluidicsnatural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsengineering and technologynanotechnologynatural sciencesbiological sciencesbiochemistrybiomoleculeslipids Programa(s) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Tema(s) ERC-CoG-2014 - ERC Consolidator Grant Convocatoria de propuestas ERC-2014-CoG Consulte otros proyectos de esta convocatoria Régimen de financiación ERC-COG - Consolidator Grant Institución de acogida THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE Aportación neta de la UEn € 1 936 431,00 Dirección TRINITY LANE THE OLD SCHOOLS CB2 1TN Cambridge Reino Unido Ver en el mapa Región East of England East Anglia Cambridgeshire CC Tipo de actividad Higher or Secondary Education Establishments Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Coste total € 1 936 431,00 Beneficiarios (1) Ordenar alfabéticamente Ordenar por aportación neta de la UE Ampliar todo Contraer todo THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE Reino Unido Aportación neta de la UEn € 1 936 431,00 Dirección TRINITY LANE THE OLD SCHOOLS CB2 1TN Cambridge Ver en el mapa Región East of England East Anglia Cambridgeshire CC Tipo de actividad Higher or Secondary Education Establishments Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Coste total € 1 936 431,00