Infrastructure for NMR, EM and X-rays for translational research

Risultati finali

Second report on the interaction with industrial parties

Report on the interaction with industrial parties (EMBL-GR)

Delivery of 370 days of access as High-end Data Acquisition to SONNMRLSF at UU
Second report on outreach activities
Start of TA to HT integrated fragment screening in Grenoble

Start of TA to HT integrated fragment screening in Grenoble (EMBL-GR)

Secondary screening of a set of derivatives around an initial hit making use of the EU-OPENSCREEN library
Delivery of 145 days of access to NMR at CEITEC
Reports on feasibility studies on the promising translational targets
Setup for lower background for diffraction data on membrane proteins
Delivery of 100 days of access as Enhanced Support to SONNMRLSF at UU
At least two articles for the general public
Report on Coordination activities with Virtual Research Environments
Storage and queuing facilities at ESRF
120 samples through “Structural Audit”
Report on novel biophysical methods
Hiring a Project Manager
Protocols and tools for using ssNMR to study protein complexes associated with cellular compartments
Report on Coordination activities with PDBe and Elixir
Delivery of 120 samples through the Structural Audit procedure at NKI
Report on a model for future structural biology access in Europe
Delivery of 305 days of High-End Data Acquisition to NMR at BMRZ
TA access to HT crystallization and crystal mounting

TA access to HT crystallization and crystal mounting (EMBL-GR)

Delivery of 75 days of access to Cryo-EM at CSIC
First report on the interaction with industrial parties

Report on the interaction with industrial parties (EMBL-GR)

Report on financial sustainability
Delivery of 2389 hours of access to Beam MX at Diamond
Second report on conferences and meetings with iNEXT representation

Second report on conferences and meetings with iNEXT representation (EMBL-HH)

Report on the organization of TDC workshops
Access to NMR probe(s) optimized for the investigation of membrane proteins (binding studies/,screening)
Start of TA to integrated screening for focused libraries at EMBL-GR/ESRF
Workflow for EM-based determination of membrane protein structures

Workflow for EM-based determination of membrane protein structures (EMBL-HD)

Delivery of 78 days of full service ‘Protein to XTAL’at EMBL-HH
Data management system for ligand and fragment screening, extending ISPyB
Protocols for membrane protein structure determination by solid-state NMR
Protocols for correlative fluorescent labeling of proteins in vitro and in situ
Delivery of 130 days of access to cryo-EM at CEITEC
Second summary report on networking activities

Second summary report on networking activities (EMBL-HH)

Draft sustainability plan
Report on the attendance to EMBO and FEBS meetings

Report on the attendance to EMBO, FEBS and similar meetings

Automatic EM Image Processing workflow in the absence of structural flexibility
Automation of the on-line SEC setups at the partner SAXS beamlines
First summary report on networking activities

First summary report on networking activities (EMBL-HH)

Integrated submission system operational
Protocols and tools for protein interactions in mammalian cells by NMR
Delivery of 144 days of access to Titan KRIO cyro-EM at NeCEN
Delivery of 76 days of full service ‘Protein to SAXS at EMBL-HH
Delivery of 192 supervised access days to EMCF at EMBL-HD
Protocols for data acquisition for correlative CLEXM
Storage and queuing facilities at Diamond
Delivery of 5450 supervised access hours to ALMF at EMBL-HD
Report on comparative analysis of data models in all disciplines and strategy for integration
Delivery of 110 days of access to NMR at RALF-NMR
Delivery of 36 days of accessto Integrated fragment screening by MX at EMBL-GR
Report on the organization of annual user meetings
Delivery of 1555 hours of access to MX beamtime at EMBL-HH
Delivery of 14 days of access to Integrated compound screening (focused libraries) by MX at EMBL-GR
Protocols for RNA sample optimization and DNA/RNA delivery into bacterial and mammalian cells. for in-cell NMR studies
Access to an integrated miniaturized HT-SEC-SAXS set-up

Access to an integrated miniaturized HT-SEC-SAXS set-up (EMBL-HH)

Management Guidelines
Delivery of 70 days of access to Integrated Macromolecular Crystallography service at EMBL-GR
Optimized protocols for reconstitution of membrane proteins

Optimized protocols for reconstitution of membrane proteins (EMBL-HH)

Automated SAXS pipeline for membrane proteins, integrated into ISPyB

Automated SAXS pipeline for membrane proteins, integrated into ISPyB (EMBL-HH)

Automated pipelines for NMR-based structure determination accessible online
Delivery of 10 days of Structural Audit to NMR at BMRZ
Delivery of 2720 hours of access to Beam MX/SAXS at SOLEIL
Start of TA to HT integrated fragment screening at Diamond
Fully automatic screening, large scale X-ray data collection pipelines
Start of TA access to HT fragment screening by NMR
Delivery of 10 days of access as Structural Audit to SONNMRLSF at UU
Delivery of 100 days of Enhanced Support to NMR at BMRZ
Delivery of 212 days of access to NMR at FVB-FMP
Report on demands of the user community
Representation on RI bodies or Committees
Protocols for preparing ssNMR samples in bacterial and mammalian cell fractions and cells
15 structure validation projects through biophysics facility
Characterization of 120 “SAXS x-audit” samples at EMBL-HH
Delivery of 346 days of High-End Data Acquisition to NMR at CERM/CIRMMP
SONICC-based centring of membrane protein crystals
CRIMS support for ligand and fragment screening

CRIMS support for ligand and fragment screening (EMBL-GR)

Data management system for a EM workflows, extending ISPyB
Fragment library distributed to partners
Delivery of 775 hours of access to SAXS beamtime at EMBL-HH
Summary report on networking activities with industry

Summary report on networking activities with industry (EMBL-GR)

First report on outreach activities
First report on conferences and meetings with iNEXT representation

First report on conferences and meetings with iNEXT representation (EMBL-HH)

Delivery of access for 60 mail-in SAXS sessions at Diamond
Delivery of 30 samples analysed for Structural Audit at CSIC

Delivery of 30 samples analysed for Structural Audit at CSIC

Optimized workflows for ligand screening by NMR
Best practice workflows for cryo electron tomography specimen preparation
Fluorophore knock in cell lines for structural studies

Fluorophore knock in cell lines for structural studies (EMBL-HD)

Protocols for cooperation with ESFRI BMS RIs
5 integrated structure validation projects through combined access in biophysics and imaging
Delivery of access to 10 screening campaigns at Diamond
Delivery of 20 projects for biophysical characterisation at NKI
Delivery of 10 days of Structural Audit to NMR at CERM/CIRMMP
Delivery of 750 hours of access to the BioMAX beamline at MAXIV
Delivery of 100 days of Enhanced Support to NMR at CERM/CIRMMP
15 structure validation projects through advanced imaging facility
Delivery of 110 days of access to cryo-EM at Diamond

Pubblicazioni

Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo

Autori: Ajasja Ljubetič, Fabio Lapenta, Helena Gradišar, Igor Drobnak, Jana Aupič, Žiga Strmšek, Duško Lainšček, Iva Hafner-Bratkovič, Andreja Majerle, Nuša Krivec, Mojca Benčina, Tomaž Pisanski, Tanja Ćirković Veličković, Adam Round, José María Carazo, Roberto Melero, Roman Jerala
Pubblicato in: Nature Biotechnology, Numero 35(11), 2017, Pagina/e 1094-1101, ISSN 1087-0156
Editore: Nature Publishing Group
DOI: 10.1038/nbt.3994

Structural Basis for Polyproline-Mediated Ribosome Stalling and Rescue by the Translation Elongation Factor EF-P

Autori: Paul Huter, Stefan Arenz, Lars V. Bock, Michael Graf, Jan Ole Frister, Andre Heuer, Lauri Peil, Agata L. Starosta, Ingo Wohlgemuth, Frank Peske, Jiří Nováček, Otto Berninghausen, Helmut Grubmüller, Tanel Tenson, Roland Beckmann, Marina V. Rodnina, Andrea C. Vaiana, Daniel N. Wilson
Pubblicato in: Molecular Cell, Numero 68/3, 2017, Pagina/e 515-527.e6, ISSN 1097-2765
Editore: Cell Press
DOI: 10.1016/j.molcel.2017.10.014

Characterization of the Conjugation Pattern in Large Polysaccharide-Protein Conjugates by NMR Spectroscopy

Autori: Stefano Giuntini, Evita Balducci, Linda Cerofolini, Enrico Ravera, Marco Fragai, Francesco Berti, Claudio Luchinat
Pubblicato in: Angewandte Chemie International Edition, Numero 56/47, 2017, Pagina/e 14997-15001, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201709274

Structural Snapshots of α-1,3-Galactosyltransferase with Native Substrates: Insight into the Catalytic Mechanism of Retaining Glycosyltransferases

Autori: David Albesa-Jové, M. Ángela Sainz-Polo, Alberto Marina, Marcelo E. Guerin
Pubblicato in: Angewandte Chemie International Edition, Numero 56/47, 2017, Pagina/e 14853-14857, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201707922

Enhanced Pru p 3 IgE-binding activity by selective free fatty acid-interaction

Autori: Pawel Dubiela, Roberta Aina, Dominika Polak, Sabine Geiselhart, Piotr Humeniuk, Barbara Bohle, Stefano Alessandri, Rebecca Del Conte, Francesca Cantini, Tomasz Borowski, Merima Bublin, Karin Hoffmann-Sommergruber
Pubblicato in: Journal of Allergy and Clinical Immunology, Numero 140/6, 2017, Pagina/e 1728-1731.e10, ISSN 0091-6749
Editore: Mosby Inc.
DOI: 10.1016/j.jaci.2017.06.016

Impact of Azidohomoalanine Incorporation on Protein Structure and Ligand Binding

Autori: Florian Lehner, Denis Kudlinzki, Christian Richter, Henrike M. Müller-Werkmeister, Katharina B. Eberl, Jens Bredenbeck, Harald Schwalbe, Robert Silvers
Pubblicato in: ChemBioChem, Numero 18/23, 2017, Pagina/e 2340-2350, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201700437

A molecular chaperone activity of CCS restores the maturation of SOD1 fALS mutants

Autori: Enrico Luchinat, Letizia Barbieri, Lucia Banci
Pubblicato in: Scientific Reports, Numero 7/1, 2017, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-017-17815-y

Structural basis for genome wide recognition of 5-bp GC motifs by SMAD transcription factors

Autori: Pau Martin-Malpartida, Marta Batet, Zuzanna Kaczmarska, Regina Freier, Tiago Gomes, Eric Aragón, Yilong Zou, Qiong Wang, Qiaoran Xi, Lidia Ruiz, Angela Vea, José A. Márquez, Joan Massagué, Maria J. Macias
Pubblicato in: Nature Communications, Numero 8/1, 2017, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-017-02054-6

Structure of outer membrane protein G in lipid bilayers

Autori: Joren S. Retel, Andrew J. Nieuwkoop, Matthias Hiller, Victoria A. Higman, Emeline Barbet-Massin, Jan Stanek, Loren B. Andreas, W. Trent Franks, Barth-Jan van Rossum, Kutti R. Vinothkumar, Lieselotte Handel, Gregorio Giuseppe de Palma, Benjamin Bardiaux, Guido Pintacuda, Lyndon Emsley, Werner Kühlbrandt, Hartmut Oschkinat
Pubblicato in: Nature Communications, Numero 8/1, 2017, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-017-02228-2

Structural and functional dissection of the DH and PH domains of oncogenic Bcr-Abl tyrosine kinase

Autori: Sina Reckel, Charlotte Gehin, Delphine Tardivon, Sandrine Georgeon, Tim Kükenshöner, Frank Löhr, Akiko Koide, Lena Buchner, Alejandro Panjkovich, Aline Reynaud, Sara Pinho, Barbara Gerig, Dmitri Svergun, Florence Pojer, Peter Güntert, Volker Dötsch, Shohei Koide, Anne-Claude Gavin, Oliver Hantschel
Pubblicato in: Nature Communications, Numero 8/1, 2017, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-017-02313-6

Atomic structural details of a protein grafted onto gold nanoparticles

Autori: Stefano Giuntini, Linda Cerofolini, Enrico Ravera, Marco Fragai, Claudio Luchinat
Pubblicato in: Scientific Reports, Numero 7/1, 2017, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-017-18109-z

Molecular mechanism for the subversion of the retromer coat by the Legionella effector RidL

Autori: Miguel Romano-Moreno, Adriana L. Rojas, Chad D. Williamson, David C. Gershlick, María Lucas, Michail N. Isupov, Juan S. Bonifacino, Matthias P. Machner, Aitor Hierro
Pubblicato in: Proceedings of the National Academy of Sciences, Numero 114/52, 2017, Pagina/e E11151-E11160, ISSN 0027-8424
Editore: National Academy of Sciences
DOI: 10.1073/pnas.1715361115

Long-range paramagnetic NMR data can provide a closer look on metal coordination in metalloproteins

Autori: Linda Cerofolini, Tommaso Staderini, Stefano Giuntini, Enrico Ravera, Marco Fragai, Giacomo Parigi, Roberta Pierattelli, Claudio Luchinat
Pubblicato in: JBIC Journal of Biological Inorganic Chemistry, Numero 23/1, 2018, Pagina/e 71-80, ISSN 0949-8257
Editore: Springer Verlag
DOI: 10.1007/s00775-017-1511-y

Intracellular metal binding and redox behavior of human DJ-1

Autori: Letizia Barbieri, Enrico Luchinat, Lucia Banci
Pubblicato in: JBIC Journal of Biological Inorganic Chemistry, Numero 23/1, 2018, Pagina/e 61-69, ISSN 0949-8257
Editore: Springer Verlag
DOI: 10.1007/s00775-017-1509-5

Dynamic control of RSK complexes by phosphoswitch-based regulation

Autori: Gergő Gógl, Beáta Biri-Kovács, Ádám L. Póti, Henrietta Vadászi, Bálint Szeder, Andrea Bodor, Gitta Schlosser, András Ács, Lilla Turiák, László Buday, Anita Alexa, László Nyitray, Attila Reményi
Pubblicato in: The FEBS Journal, Numero 285/1, 2018, Pagina/e 46-71, ISSN 1742-464X
Editore: Blackwell Publishing Inc.
DOI: 10.1111/febs.14311

Combined 1H-Detected Solid-State NMR Spectroscopy and Electron Cryotomography to Study Membrane Proteins across Resolutions in Native Environments

Autori: Baker LA, Sinnige T, Schellenberger P, de Keyzer J, Siebert CA, Driessen AJM, Baldus M, Grünewald K
Pubblicato in: Structure, Numero 26/1, 2018, Pagina/e 161-170.e3, ISSN 0969-2126
Editore: Elsevier

Structural basis of RNA polymerase III transcription initiation

Autori: Guillermo Abascal-Palacios, Ewan Phillip Ramsay, Fabienne Beuron, Edward Morris, Alessandro Vannini
Pubblicato in: Nature, Numero 553/7688, 2018, Pagina/e 301-306, ISSN 0028-0836
Editore: Nature Publishing Group
DOI: 10.1038/nature25441

1 H NMR Spectroscopy of [FeFe] Hydrogenase: Insight into the Electronic Structure of the Active Site

Autori: Sigrun Rumpel, Enrico Ravera, Constanze Sommer, Edward Reijerse, Christophe Farès, Claudio Luchinat, Wolfgang Lubitz
Pubblicato in: Journal of the American Chemical Society, Numero 140/1, 2017, Pagina/e 131-134, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/jacs.7b11196

Structural plasticity of the N-terminal capping helix of the TPR domain of kinesin light chain

Autori: The Quyen Nguyen, Mélanie Chenon, Fernando Vilela, Christophe Velours, Magali Aumont-Nicaise, Jessica Andreani, Paloma F. Varela, Paola Llinas, Julie Ménétrey
Pubblicato in: PLOS ONE, Numero October 16, 2017, 2017, Pagina/e 1-20, ISSN 1932-6203
Editore: Public Library of Science
DOI: 10.1371/journal.pone.0186354.s004

Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research

Autori: Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
Pubblicato in: Structure, Numero 26, 2018, Pagina/e 345-355, ISSN 0969-2126
Editore: Cell Press
DOI: 10.1016/j.str.2018.01.007

CHROMIXS: automatic and interactive analysis of chromatography-coupled small-angle X-ray scattering data

Autori: Alejandro Panjkovich, Dmitri I Svergun
Pubblicato in: Bioinformatics, Numero 28 December 2017, 2017, Pagina/e 1-3, ISSN 1367-4803
Editore: Oxford University Press
DOI: 10.1093/bioinformatics/btx846

Automated harvesting and processing of protein crystals through laser photoablation

Autori: Ulrich Zander, Guillaume Hoffmann, Irina Cornaciu, Jean-Pierre Marquette, Gergely Papp, Christophe Landret, Gaël Seroul, Jérémy Sinoir, Martin Röwer, Frank Felisaz, Sonia Rodriguez-Puente, Vincent Mariaule, Peter Murphy, Magali Mathieu, Florent Cipriani, José Antonio Márquez
Pubblicato in: Acta Crystallographica Section D Structural Biology, Numero 72/4, 2016, Pagina/e 454-466, ISSN 2059-7983
Editore: John Wiley & Sons Ltd
DOI: 10.1107/S2059798316000954

RavN is a member of a previously unrecognized group of Legionella pneumophila E3 ubiquitin ligases

Autori: Yi-Han Lin, María Lucas, Timothy R. Evans, Guillermo Abascal-Palacios, Alexandra G. Doms, Nicole A. Beauchene, Adriana L. Rojas, Aitor Hierro, Matthias P. Machner
Pubblicato in: PLOS Pathogens, Numero 14/2, 2018, Pagina/e e1006897, ISSN 1553-7374
Editore: PLOS
DOI: 10.1371/journal.ppat.1006897

An allosteric binding site of the α7 nicotinic acetylcholine receptor revealed in a humanized acetylcholine-binding protein

Autori: Florian Delbart, Marijke Brams, Fabian Gruss, Sam Noppen, Steve Peigneur, Sandro Boland, Patrick Chaltin, Jose Brandao-Neto, Frank von Delft, Wouter G. Touw, Robbie P. Joosten, Sandra Liekens, Jan Tytgat, Chris Ulens
Pubblicato in: Journal of Biological Chemistry, Numero 293/7, 2018, Pagina/e 2534-2545, ISSN 0021-9258
Editore: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.M117.815316

Evaluation of the Stability of DNA i-Motifs in the Nuclei of Living Mammalian Cells

Autori: Simon Dzatko, Michaela Krafcikova, Robert Hänsel-Hertsch, Tomas Fessl, Radovan Fiala, Tomas Loja, Daniel Krafcik, Jean-Louis Mergny, Silvie Foldynova-Trantirkova, Lukas Trantirek
Pubblicato in: Angewandte Chemie International Edition, Numero 57/8, 2018, Pagina/e 2165-2169, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201712284

A family of unconventional deubiquitinases with modular chain specificity determinants

Autori: Thomas Hermanns, Christian Pichlo, Ilka Woiwode, Karsten Klopffleisch, Katharina F. Witting, Huib Ovaa, Ulrich Baumann, Kay Hofmann
Pubblicato in: Nature Communications, Numero 9/1, 2018, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-018-03148-5

Multi-channel in situ dynamic light scattering instrumentation enhancing biological small-angle X-ray scattering experiments at the PETRA III beamline P12

Autori: Sven Falke, Karsten Dierks, Clement Blanchet, Melissa Graewert, Florent Cipriani, Rob Meijers, Dmitri Svergun, Christian Betzel
Pubblicato in: Journal of Synchrotron Radiation, Numero 25/2, 2018, Pagina/e 361-372, ISSN 1600-5775
Editore: IUCr
DOI: 10.1107/S1600577517017568

Homology-based hydrogen bond information improves crystallographic structures in the PDB

Autori: Bart van Beusekom, Wouter G. Touw, Mahidhar Tatineni, Sandeep Somani, Gunaretnam Rajagopal, Jinquan Luo, Gary L. Gilliland, Anastassis Perrakis, Robbie P. Joosten
Pubblicato in: Protein Science, Numero 27/3, 2018, Pagina/e 798-808, ISSN 0961-8368
Editore: Cold Spring Harbor Laboratory Press
DOI: 10.1002/pro.3353

Solution NMR structure of yeast Rcf1, a protein involved in respiratory supercomplex formation

Autori: Shu Zhou, Pontus Pettersson, Jingjing Huang, Johannes Sjöholm, Dan Sjöstrand, Régis Pomès, Martin Högbom, Peter Brzezinski, Lena Mäler, Pia Ädelroth
Pubblicato in: Proceedings of the National Academy of Sciences, Numero 115/12, 2018, Pagina/e 3048-3053, ISSN 0027-8424
Editore: National Academy of Sciences
DOI: 10.1073/pnas.1712061115

Multispecific Substrate Recognition in a Proton-Dependent Oligopeptide Transporter.

Autori: Martinez Molledo M, Quistgaard EM, Flayhan A, Pieprzyk J, Löw C
Pubblicato in: Structure, Numero Mar 6;26(3), 2018, Pagina/e 467-476, ISSN 0969-2126
Editore: Elsevier

A multidisciplinary study of 3-(β- d -glucopyranosyl)-5-substituted-1,2,4-triazole derivatives as glycogen phosphorylase inhibitors: Computation, synthesis, crystallography and kinetics reveal new potent inhibitors

Autori: Sándor Kun, Jaida Begum, Efthimios Kyriakis, Evgenia C.V. Stamati, Thomas A. Barkas, Eszter Szennyes, Éva Bokor, Katalin E. Szabó, George A. Stravodimos, Ádám Sipos, Tibor Docsa, Pál Gergely, Colin Moffatt, Myrto S. Patraskaki, Maria C. Kokolaki, Alkistis Gkerdi, Vassiliki T. Skamnaki, Demetres D. Leonidas, László Somsák, Joseph M. Hayes
Pubblicato in: European Journal of Medicinal Chemistry, Numero 147, 2018, Pagina/e 266-278, ISSN 0223-5234
Editore: Elsevier BV
DOI: 10.1016/j.ejmech.2018.01.095

Structural model of human dUTPase in complex with a novel proteinaceous inhibitor

Autori: Kinga Nyíri, Haydyn D. T. Mertens, Borbála Tihanyi, Gergely N. Nagy, Bianka Kőhegyi, Judit Matejka, Matthew J. Harris, Judit E. Szabó, Veronika Papp-Kádár, Veronika Németh-Pongrácz, Olivér Ozohanics, Károly Vékey, Dmitri I. Svergun, Antoni J. Borysik, Beáta G. Vértessy
Pubblicato in: Scientific Reports, Numero 8/1, 2018, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-018-22145-8

Structures of Teneurin adhesion receptors reveal an ancient fold for cell-cell interaction

Autori: Verity A. Jackson, Dimphna H. Meijer, Maria Carrasquero, Laura S. van Bezouwen, Edward D. Lowe, Colin Kleanthous, Bert J. C. Janssen, Elena Seiradake
Pubblicato in: Nature Communications, Numero 9/1, 2018, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-018-03460-0

Structural changes of TasA in biofilm formation of Bacillus subtilis

Autori: Anne Diehl, Yvette Roske, Linda Ball, Anup Chowdhury, Matthias Hiller, Noel Molière, Regina Kramer, Daniel Stöppler, Catherine L. Worth, Brigitte Schlegel, Martina Leidert, Nils Cremer, Natalja Erdmann, Daniel Lopez, Heike Stephanowitz, Eberhard Krause, Barth-Jan van Rossum, Peter Schmieder, Udo Heinemann, Kürşad Turgay, Ümit Akbey, Hartmut Oschkinat
Pubblicato in: Proceedings of the National Academy of Sciences, Numero 115/13, 2018, Pagina/e 3237-3242, ISSN 0027-8424
Editore: National Academy of Sciences
DOI: 10.1073/pnas.1718102115

1H, 13C, and 15N chemical shift assignments of a G-quadruplex forming sequence within the KRAS proto-oncogene promoter region

Autori: Julien Marquevielle, M. V. Vasantha Kumar, Jean-Louis Mergny, Gilmar F. Salgado
Pubblicato in: Biomolecular NMR Assignments, Numero 12/1, 2018, Pagina/e 123-127, ISSN 1874-2718
Editore: Springer Verlag
DOI: 10.1007/s12104-017-9793-0

A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein–RNA Interactions

Autori: Po-chia Chen, Pawel Masiewicz, Vladimir Rybin, Dmitri Svergun, Janosch Hennig
Pubblicato in: ACS Combinatorial Science, Numero 20/4, 2018, Pagina/e 197-202, ISSN 2156-8952
Editore: American Chemical Society
DOI: 10.1021/acscombsci.8b00007

Structural characterization of the intrinsically disordered domain of Mycobacterium tuberculosis protein tyrosine kinase A

Autori: Anna Niesteruk, Marie Hutchison, Sridhar Sreeramulu, Hendrik R.A. Jonker, Christian Richter, Rupert Abele, Christoph Bock, Harald Schwalbe
Pubblicato in: FEBS Letters, Numero 592/7, 2018, Pagina/e 1233-1245, ISSN 0014-5793
Editore: Elsevier BV
DOI: 10.1002/1873-3468.13022

RPAP3 provides a flexible scaffold for coupling HSP90 to the human R2TP co-chaperone complex

Autori: Fabrizio Martino, Mohinder Pal, Hugo Muñoz-Hernández, Carlos F. Rodríguez, Rafael Núñez-Ramírez, David Gil-Carton, Gianluca Degliesposti, J. Mark Skehel, S. Mark Roe, Chrisostomos Prodromou, Laurence H. Pearl, Oscar Llorca
Pubblicato in: Nature Communications, Numero 9/1, 2018, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-018-03942-1

The cysteine-reactive small molecule ebselen facilitates effective SOD1 maturation

Autori: Michael J. Capper, Gareth S. A. Wright, Letizia Barbieri, Enrico Luchinat, Eleonora Mercatelli, Luke McAlary, Justin J. Yerbury, Paul M. O’Neill, Svetlana V. Antonyuk, Lucia Banci, S. Samar Hasnain
Pubblicato in: Nature Communications, Numero 9/1, 2018, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-018-04114-x

Functional interaction of low-homology FRPs from different cyanobacteria with Synechocystis OCP

Autori: Yury B. Slonimskiy, Eugene G. Maksimov, Evgeny P. Lukashev, Marcus Moldenhauer, Cy M. Jeffries, Dmitri I. Svergun, Thomas Friedrich, Nikolai N. Sluchanko
Pubblicato in: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Numero 1859/5, 2018, Pagina/e 382-393, ISSN 0005-2728
Editore: Elsevier BV
DOI: 10.1016/j.bbabio.2018.03.001

Integrated beamline control and data acquisition for small-angle X-ray scattering at the P12 BioSAXS beamline at PETRAIII storage ring DESY

Autori: Nelly R. Hajizadeh, Daniel Franke, Dmitri I. Svergun
Pubblicato in: Journal of Synchrotron Radiation, Numero 25/3, 2018, Pagina/e 906-914, ISSN 1600-5775
Editore: IUCr
DOI: 10.1107/S1600577518005398

Consensus Bayesian assessment of protein molecular mass from solution X-ray scattering data

Autori: Nelly R. Hajizadeh, Daniel Franke, Cy M. Jeffries, Dmitri I. Svergun
Pubblicato in: Scientific Reports, Numero 8/1, 2018, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-018-25355-2

Structure of the hexagonal surface layer on Caulobacter crescentus cells

Autori: Tanmay A. M. Bharat, Danguole Kureisaite-Ciziene, Gail G. Hardy, Ellen W. Yu, Jessica M. Devant, Wim J. H. Hagen, Yves V. Brun, John A. G. Briggs, Jan Löwe
Pubblicato in: Nature Microbiology, Numero 2, 2017, Pagina/e 17059, ISSN 2058-5276
Editore: Springer Nature
DOI: 10.1038/nmicrobiol.2017.59

The structure of FIV reverse transcriptase and its implications for non-nucleoside inhibitor resistance

Autori: Meytal Galilee, Akram Alian
Pubblicato in: PLOS Pathogens, Numero 14/1, 2018, Pagina/e e1006849, ISSN 1553-7374
Editore: PLOS
DOI: 10.1371/journal.ppat.1006849

NMR-Based Plasma Metabolomics at Set Intervals in Newborn Dairy Calves with Severe Sepsis

Autori: Abdullah Basoglu, Ismail Sen, Gaia Meoni, Leonardo Tenori, Amir Naseri
Pubblicato in: Mediators of Inflammation, Numero 2018, 2018, Pagina/e 1-12, ISSN 0962-9351
Editore: Hindawi Publishing Corporation
DOI: 10.1155/2018/8016510

iNEXT: a European facility network to stimulate translational structural biology

Autori: iNEXT Consortium
Pubblicato in: FEBS Letters, 2018, ISSN 0014-5793
Editore: Elsevier BV
DOI: 10.1002/1873-3468.13062

Complete assignment of Ala, Ile, Leu, Met and Val methyl groups of human blood group A and B glycosyltransferases using lanthanide-induced pseudocontact shifts and methyl–methyl NOESY

Autori: Friedemann Flügge, Thomas Peters
Pubblicato in: Journal of Biomolecular NMR, Numero 70/4, 2018, Pagina/e 245-259, ISSN 0925-2738
Editore: Kluwer Academic Publishers
DOI: 10.1007/s10858-018-0183-4

Engineering l -asparaginase for spontaneous formation of calcium phosphate bioinspired microreactors

Autori: Alexandra Louka, Irina Matlahov, Stefano Giuntini, Linda Cerofolini, Andrea Cavallo, Serena Pillozzi, Enrico Ravera, Marco Fragai, Annarosa Arcangeli, Ayyalusamy Ramamoorthy, Gil Goobes, Claudio Luchinat
Pubblicato in: Physical Chemistry Chemical Physics, Numero 20/18, 2018, Pagina/e 12719-12726, ISSN 1463-9076
Editore: Royal Society of Chemistry
DOI: 10.1039/c8cp00419f

Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S

Autori: Beatriz Trastoy, Erik Klontz, Jared Orwenyo, Alberto Marina, Lai-Xi Wang, Eric J. Sundberg, Marcelo E. Guerin
Pubblicato in: Nature Communications, Numero 9/1, 2018, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-018-04300-x

Targeting RNA structure in SMN2 reverses spinal muscular atrophy molecular phenotypes

Autori: Amparo Garcia-Lopez, Francesca Tessaro, Hendrik R. A. Jonker, Anna Wacker, Christian Richter, Arnaud Comte, Nikolaos Berntenis, Roland Schmucki, Klas Hatje, Olivier Petermann, Gianpaolo Chiriano, Remo Perozzo, Daniel Sciarra, Piotr Konieczny, Ignacio Faustino, Guy Fournet, Modesto Orozco, Ruben Artero, Friedrich Metzger, Martin Ebeling, Peter Goekjian, Benoît Joseph, Harald Schwalbe, Leonardo Sca
Pubblicato in: Nature Communications, Numero 9/1, 2018, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-018-04110-1

Crystal structure of thebaine 6-O-demethylase from the morphine biosynthesis pathway

Autori: Anna Kluza, Ewa Niedzialkowska, Katarzyna Kurpiewska, Zuzanna Wojdyla, Matthew Quesne, Ewa Kot, Przemyslaw J. Porebski, Tomasz Borowski
Pubblicato in: Journal of Structural Biology, Numero 202/3, 2018, Pagina/e 229-235, ISSN 1047-8477
Editore: Academic Press
DOI: 10.1016/j.jsb.2018.01.007

Enabling STD-NMR fragment screening using stabilized native GPCR: A case study of adenosine receptor

Autori: Sébastien Igonet, Claire Raingeval, Erika Cecon, Maja Pučić-Baković, Gordan Lauc, Olivier Cala, Maciej Baranowski, Javier Perez, Ralf Jockers, Isabelle Krimm, Anass Jawhari
Pubblicato in: Scientific Reports, Numero 8/1, 2018, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-018-26113-0

Generation and validation of homozygous fluorescent knock-in cells using CRISPR–Cas9 genome editing

Autori: Birgit Koch, Bianca Nijmeijer, Moritz Kueblbeck, Yin Cai, Nike Walther, Jan Ellenberg
Pubblicato in: Nature Protocols, Numero 13/6, 2018, Pagina/e 1465-1487, ISSN 1754-2189
Editore: Nature Publishing Group
DOI: 10.1038/nprot.2018.042

Quantitative mapping of fluorescently tagged cellular proteins using FCS-calibrated four-dimensional imaging

Autori: Antonio Z Politi, Yin Cai, Nike Walther, M Julius Hossain, Birgit Koch, Malte Wachsmuth, Jan Ellenberg
Pubblicato in: Nature Protocols, Numero 13/6, 2018, Pagina/e 1445-1464, ISSN 1754-2189
Editore: Nature Publishing Group
DOI: 10.1038/nprot.2018.040

Insights into catalysis and function of phosphoribosyl-linked serine ubiquitination

Autori: Sissy Kalayil, Sagar Bhogaraju, Florian Bonn, Donghyuk Shin, Yaobin Liu, Ninghai Gan, Jérôme Basquin, Paolo Grumati, Zhao-Qing Luo, Ivan Dikic
Pubblicato in: Nature, Numero 557/7707, 2018, Pagina/e 734-738, ISSN 0028-0836
Editore: Nature Publishing Group
DOI: 10.1038/s41586-018-0145-8

Correlative microscopy for structural microbiology

Autori: Stuart C Howes, Roman I Koning, Abraham J Koster
Pubblicato in: Current Opinion in Microbiology, Numero 43, 2018, Pagina/e 132-138, ISSN 1369-5274
Editore: Elsevier BV
DOI: 10.1016/j.mib.2018.01.009

Advances in the cellular structural biology of nucleic acids

Autori: Ilektra-Chara Giassa, Jan Rynes, Tomas Fessl, Silvie Foldynova-Trantirkova, Lukas Trantirek
Pubblicato in: FEBS Letters, Numero 592/12, 2018, Pagina/e 1997-2011, ISSN 0014-5793
Editore: Elsevier BV
DOI: 10.1002/1873-3468.13054

Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions

Autori: Daniel Franke, Cy M. Jeffries, Dmitri I. Svergun
Pubblicato in: Biophysical Journal, Numero 114/11, 2018, Pagina/e 2485-2492, ISSN 0006-3495
Editore: Biophysical Society
DOI: 10.1016/j.bpj.2018.04.018

Biophysical and structural characterization of the putative nickel chaperone CooT from Carboxydothermus hydrogenoformans

Autori: M. Alfano, J. Pérard, R. Miras, P. Catty, C. Cavazza
Pubblicato in: JBIC Journal of Biological Inorganic Chemistry, Numero 23/5, 2018, Pagina/e 809-817, ISSN 0949-8257
Editore: Springer Verlag
DOI: 10.1007/s00775-018-1576-2

Influence of pH on structure and stability of the sweet protein MNEI

Autori: Roberta Spadaccini, Serena Leone, Michele Fortunato Rega, Christian Richter, Delia Picone
Pubblicato in: FEBS Letters, Numero Sept 29, 2016, 2016, ISSN 0014-5793
Editore: Elsevier BV
DOI: 10.1002/1873-3468.12437

Cross-membranes orchestrate compartmentalization and morphogenesis in Streptomyces

Autori: Katherine Celler, Roman I. Koning, Joost Willemse, Abraham J. Koster, Gilles P. van Wezel
Pubblicato in: Nature Communications, Numero 7, 2016, Pagina/e ncomms11836, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/ncomms11836

Subcompartmentalization by cross-membranes during early growth of Streptomyces hyphae

Autori: Paula Yagüe, Joost Willemse, Roman I. Koning, Beatriz Rioseras, María T. López-García, Nathaly Gonzalez-Quiñonez, Carmen Lopez-Iglesias, Pavel V. Shliaha, Adelina Rogowska-Wrzesinska, Abraham J. Koster, Ole N. Jensen, Gilles P. van Wezel, Ángel Manteca
Pubblicato in: Nature Communications, Numero 7, 2016, Pagina/e 12467, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/ncomms12467

Structural Characterization of the Interaction of the Fibroblast Growth Factor Receptor with a Small Molecule Allosteric Inhibitor

Autori: Franziska Kappert, Sridhar Sreeramulu, Hendrik R. A. Jonker, Christian Richter, Vladimir V. Rogov, Ewgenij Proschak, Bruno Hargittay, Krishna Saxena, Harald Schwalbe
Pubblicato in: Chemistry - A European Journal, Numero 24/31, 2018, Pagina/e 7861-7865, ISSN 0947-6539
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/chem.201801770

Dynamic kinetochore size regulation promotes microtubule capture and chromosome biorientation in mitosis

Autori: Carlos Sacristan, Misbha Ud Din Ahmad, Jenny Keller, Job Fermie, Vincent Groenewold, Eelco Tromer, Alexander Fish, Roberto Melero, José María Carazo, Judith Klumperman, Andrea Musacchio, Anastassis Perrakis, Geert JPL Kops
Pubblicato in: Nature Cell Biology, Numero 20/7, 2018, Pagina/e 800-810, ISSN 1465-7392
Editore: Nature Publishing Group
DOI: 10.1038/s41556-018-0130-3

Direct shape determination of intermediates in evolving macromolecular solutions from small-angle scattering data

Autori: Petr V. Konarev, Dmitri I. Svergun
Pubblicato in: IUCrJ, Numero 5/4, 2018, Pagina/e 402-409, ISSN 2052-2525
Editore: IUCrJ
DOI: 10.1107/S2052252518005900

Smaller capillaries improve the small-angle X-ray scattering signal and sample consumption for biomacromolecular solutions

Autori: Martin A. Schroer, Clement E. Blanchet, Andrey Yu. Gruzinov, Melissa A. Gräwert, Martha E. Brennich, Nelly R. Hajizadeh, Cy M. Jeffries, Dmitri I. Svergun
Pubblicato in: Journal of Synchrotron Radiation, Numero 25/4, 2018, Pagina/e 1113-1122, ISSN 1600-5775
Editore: IUCrJ
DOI: 10.1107/S1600577518007907

Paradoxically, Most Flexible Ligand Binds Most Entropy-Favored: Intriguing Impact of Ligand Flexibility and Solvation on Drug–Kinase Binding

Autori: Barbara Wienen-Schmidt, Hendrik R. A. Jonker, Tobias Wulsdorf, Hans-Dieter Gerber, Krishna Saxena, Denis Kudlinzki, Sridhar Sreeramulu, Giacomo Parigi, Claudio Luchinat, Andreas Heine, Harald Schwalbe, Gerhard Klebe
Pubblicato in: Journal of Medicinal Chemistry, Numero 61/14, 2018, Pagina/e 5922-5933, ISSN 0022-2623
Editore: American Chemical Society
DOI: 10.1021/acs.jmedchem.8b00105

The domain architecture of PtkA, the first tyrosine kinase from Mycobacterium tuberculosis , differs from the conventional kinase architecture

Autori: Anna Niesteruk, Hendrik R. A. Jonker, Christian Richter, Verena Linhard, Sridhar Sreeramulu, Harald Schwalbe
Pubblicato in: Journal of Biological Chemistry, Numero 293/30, 2018, Pagina/e 11823-11836, ISSN 0021-9258
Editore: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.RA117.000120

Visualization of translation termination intermediates trapped by the Apidaecin 137 peptide during RF3-mediated recycling of RF1

Autori: Michael Graf, Paul Huter, Cristina Maracci, Miroslav Peterek, Marina V. Rodnina, Daniel N. Wilson
Pubblicato in: Nature Communications, Numero 9/1, 2018, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-018-05465-1

Recognition of Complex Core-Fucosylated N-Glycans by a Mini Lectin

Autori: Aurore Cabanettes, Lukas Perkams, Carolina Spies, Carlo Unverzagt, Annabelle Varrot
Pubblicato in: Angewandte Chemie International Edition, Numero 57/32, 2018, Pagina/e 10178-10181, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201805165

1H-detected solid-state NMR of proteins entrapped in bioinspired silica: a new tool for biomaterials characterization

Autori: Enrico Ravera, Linda Cerofolini, Tommaso Martelli, Alexandra Louka, Marco Fragai, Claudio Luchinat
Pubblicato in: Scientific Reports, Numero 6, 2016, Pagina/e 27851, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/srep27851

Aromatic Cluster Sensor of Protein Folding: Near-UV Electronic Circular Dichroism Bands Assigned to Fold Compactness

Autori: Viktor Farkas, Imre Jákli, Gábor K. Tóth, András Perczel
Pubblicato in: Chemistry - A European Journal, Numero 22/39, 2016, Pagina/e 13871-13883, ISSN 0947-6539
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/chem.201602455

Emergence of a Homo sapiens-specific gene family and chromosome 16p11.2 CNV susceptibility

Autori: Xander Nuttle, Giuliana Giannuzzi, Michael H. Duyzend, Joshua G. Schraiber, Iñigo Narvaiza, Peter H. Sudmant, Osnat Penn, Giorgia Chiatante, Maika Malig, John Huddleston, Chris Benner, Francesca Camponeschi, Simone Ciofi-Baffoni, Holly A. F. Stessman, Maria C. N. Marchetto, Laura Denman, Lana Harshman, Carl Baker, Archana Raja, Kelsi Penewit, Nicolette Janke, W. Joyce Tang, Mario Ventura, Lucia B
Pubblicato in: Nature, Numero 536/7615, 2016, Pagina/e 205-209, ISSN 0028-0836
Editore: Nature Publishing Group
DOI: 10.1038/nature19075

Mitochondrial Bol1 and Bol3 function as assembly factors for specific iron-sulfur proteins

Autori: Marta A Uzarska, Veronica Nasta, Benjamin D Weiler, Farah Spantgar, Simone Ciofi-Baffoni, Maria Rosaria Saviello, Leonardo Gonnelli, Ulrich Mühlenhoff, Lucia Banci, Roland Lill
Pubblicato in: eLife, Numero 5, 2016, ISSN 2050-084X
Editore: eLife Sciences Publications
DOI: 10.7554/eLife.16673

Plasma metabolomics in calves with acute bronchopneumonia

Autori: Abdullah Basoglu, Nuri Baspinar, Leonardo Tenori, Alessia Vignoli, Ramazan Yildiz
Pubblicato in: Metabolomics, Numero 12/8, 2016, ISSN 1573-3882
Editore: Springer Verlag
DOI: 10.1007/s11306-016-1074-x

EGFR Dynamics Change during Activation in Native Membranes as Revealed by NMR

Autori: Mohammed Kaplan, Siddarth Narasimhan, Cecilia de Heus, Deni Mance, Sander van Doorn, Klaartje Houben, Dušan Popov-Čeleketić, Reinier Damman, Eugene A. Katrukha, Purvi Jain, Willie J.C. Geerts, Albert J.R. Heck, Gert E. Folkers, Lukas C. Kapitein, Simone Lemeer, Paul M.P. van Bergen en Henegouwen, Marc Baldus
Pubblicato in: Cell, Numero 167, 2016, ISSN 0092-8674
Editore: Cell Press
DOI: 10.1016/j.cell.2016.10.038

Structural analysis of a signal peptide inside the ribosome tunnel by DNP MAS NMR

Autori: S. Lange, W. T. Franks, N. Rajagopalan, K. Do ring, M. A. Geiger, A. Linden, B.-J. van Rossum, G. Kramer, B. Bukau, H. Oschkinat
Pubblicato in: Science Advances, Numero 2/8, 2016, Pagina/e e1600379-e1600379, ISSN 2375-2548
Editore: AAAS
DOI: 10.1126/sciadv.1600379

Resolution of ab initio shapes determined from small-angle scattering

Autori: Anne T. Tuukkanen, Gerard J. Kleywegt, Dmitri I. Svergun
Pubblicato in: IUCrJ, Numero 3/6, 2016, Pagina/e 440-447, ISSN 2052-2525
Editore: International Union of Crystallography
DOI: 10.1107/S2052252516016018

Studying assembly of the BAM complex in native membranes by cellular solid-state NMR spectroscopy

Autori: Cecilia Pinto, Deni Mance, Manon Julien, Mark Daniels, Markus Weingarth, Marc Baldus
Pubblicato in: Journal of Structural Biology, 2017, ISSN 1047-8477
Editore: Academic Press
DOI: 10.1016/j.jsb.2017.11.015

Experimental and computational framework for a dynamic protein atlas of human cell division

Autori: Yin Cai, M. Julius Hossain, Jean-Karim Hériché, Antonio Z. Politi, Nike Walther, Birgit Koch, Malte Wachsmuth, Bianca Nijmeijer, Moritz Kueblbeck, Marina Martinic-Kavur, Rene Ladurner, Stephanie Alexander, Jan-Michael Peters, Jan Ellenberg
Pubblicato in: Nature, Numero 561/7723, 2018, Pagina/e 411-415, ISSN 0028-0836
Editore: Nature Publishing Group
DOI: 10.1038/s41586-018-0518-z

Formation of the β-barrel assembly machinery complex in lipid bilayers as seen by solid-state NMR

Autori: Cecilia Pinto, Deni Mance, Tessa Sinnige, Mark Daniëls, Markus Weingarth, Marc Baldus
Pubblicato in: Nature Communications, Numero 9/1, 2018, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-018-06466-w

Insight into small molecule binding to the neonatal Fc receptor by X-ray crystallography and 100 kHz magic-angle-spinning NMR

Autori: Daniel Stöppler, Alex Macpherson, Susanne Smith-Penzel, Nicolas Basse, Fabien Lecomte, Hervé Deboves, Richard D. Taylor, Tim Norman, John Porter, Lorna C. Waters, Marta Westwood, Ben Cossins, Katharine Cain, James White, Robert Griffin, Christine Prosser, Sebastian Kelm, Amy H. Sullivan, David Fox, Mark D. Carr, Alistair Henry, Richard Taylor, Beat H. Meier, Hartmut Oschkinat, Alastair D. Lawson
Pubblicato in: PLOS Biology, Numero 16/5, 2018, Pagina/e e2006192, ISSN 1545-7885
Editore: PLoS
DOI: 10.1371/journal.pbio.2006192

Structural basis for antibiotic resistance mediated by the Bacillus subtilis ABCF ATPase VmlR

Autori: Caillan Crowe-McAuliffe, Michael Graf, Paul Huter, Hiraku Takada, Maha Abdelshahid, Jiří Nováček, Victoriia Murina, Gemma C. Atkinson, Vasili Hauryliuk, Daniel N. Wilson
Pubblicato in: Proceedings of the National Academy of Sciences, Numero 115/36, 2018, Pagina/e 8978-8983, ISSN 0027-8424
Editore: National Academy of Sciences
DOI: 10.1073/pnas.1808535115

Multivalency regulates activity in an intrinsically disordered transcription factor

Autori: Sarah Clark, Janette B Myers, Ashleigh King, Radovan Fiala, Jiri Novacek, Grant Pearce, Jörg Heierhorst, Steve L Reichow, Elisar J Barbar
Pubblicato in: eLife, Numero 7, 2018, ISSN 2050-084X
Editore: eLife Sciences Publications
DOI: 10.7554/eLife.36258

Urease Inhibition in the Presence of N -( n -Butyl)thiophosphoric Triamide, a Suicide Substrate: Structure and Kinetics

Autori: Luca Mazzei, Michele Cianci, Umberto Contaldo, Francesco Musiani, Stefano Ciurli
Pubblicato in: Biochemistry, Numero 56/40, 2017, Pagina/e 5391-5404, ISSN 0006-2960
Editore: American Chemical Society
DOI: 10.1021/acs.biochem.7b00750

Structural and functional studies of the metalloregulator Fur identify a promoter-binding mechanism and its role in Francisella tularensis virulence

Autori: J. Pérard, S. Nader, M. Levert, L. Arnaud, P. Carpentier, C. Siebert, F. Blanquet, C. Cavazza, P. Renesto, D. Schneider, M. Maurin, J. Coves, S. Crouzy, I. Michaud-Soret
Pubblicato in: Communications Biology, Numero 1/1, 2018, ISSN 2399-3642
Editore: Springer Nature
DOI: 10.1038/s42003-018-0095-6

OCP–FRP protein complex topologies suggest a mechanism for controlling high light tolerance in cyanobacteria

Autori: Nikolai N. Sluchanko, Yury B. Slonimskiy, Evgeny A. Shirshin, Marcus Moldenhauer, Thomas Friedrich, Eugene G. Maksimov
Pubblicato in: Nature Communications, Numero 9/1, 2018, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-018-06195-0

The Regulation of Axon Diameter: From Axonal Circumferential Contractility to Activity-Dependent Axon Swelling

Autori: Ana Rita Costa, Rita Pinto-Costa, Sara Castro Sousa, Mónica Mendes Sousa
Pubblicato in: Frontiers in Molecular Neuroscience, Numero 11, 2018, ISSN 1662-5099
Editore: Frontiers Research Foundation
DOI: 10.3389/fnmol.2018.00319

Rapid access to RNA resonances by proton-detected solid-state NMR at >100 kHz MAS

Autori: Alexander Marchanka, Jan Stanek, Guido Pintacuda, Teresa Carlomagno
Pubblicato in: Chemical Communications, Numero 54/65, 2018, Pagina/e 8972-8975, ISSN 1359-7345
Editore: Royal Society of Chemistry
DOI: 10.1039/c8cc04437f

Architecture of the complete oxygen-sensing FixL-FixJ two-component signal transduction system

Autori: Gareth S. A. Wright, Akane Saeki, Takaaki Hikima, Yoko Nishizono, Tamao Hisano, Misaki Kamaya, Kohei Nukina, Hideo Nishitani, Hiro Nakamura, Masaki Yamamoto, Svetlana V. Antonyuk, S. Samar Hasnain, Yoshitsugu Shiro, Hitomi Sawai
Pubblicato in: Science Signaling, Numero 11/525, 2018, Pagina/e eaaq0825, ISSN 1937-9145
Editore: American Association for the Advancement of Science
DOI: 10.1126/scisignal.aaq0825

Disordered Peptides Looking for Their Native Environment: Structural Basis of CB1 Endocannabinoid Receptor Binding to Pepcans

Autori: Alessandro Emendato, Remo Guerrini, Erika Marzola, Hans Wienk, Rolf Boelens, Serena Leone, Delia Picone
Pubblicato in: Frontiers in Molecular Biosciences, Numero 5, 2018, ISSN 2296-889X
Editore: Frontiers
DOI: 10.3389/fmolb.2018.00100

NVP-BHG712: Effects of Regioisomers on the Affinity and Selectivity toward the EPHrin Family

Autori: Alix Tröster, Stephanie Heinzlmeir, Benedict-Tilman Berger, Santosh L. Gande, Krishna Saxena, Sridhar Sreeramulu, Verena Linhard, Amir H. Nasiri, Michael Bolte, Susanne Müller, Bernhard Kuster, Guillaume Médard, Denis Kudlinzki, Harald Schwalbe
Pubblicato in: ChemMedChem, Numero 13/16, 2018, Pagina/e 1629-1633, ISSN 1860-7179
Editore: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/cmdc.201800398

Structural basis of RNA polymerase I stalling at UV light-induced DNA damage

Autori: Marta Sanz-Murillo, Jun Xu, Georgiy A. Belogurov, Olga Calvo, David Gil-Carton, María Moreno-Morcillo, Dong Wang, Carlos Fernández-Tornero
Pubblicato in: Proceedings of the National Academy of Sciences, Numero 115/36, 2018, Pagina/e 8972-8977, ISSN 0027-8424
Editore: National Academy of Sciences
DOI: 10.1073/pnas.1802626115

On the Implication of Water on Fragment-to-Ligand Growth in Kinase Binding Thermodynamics

Autori: Barbara Wienen-Schmidt, Tobias Wulsdorf, Hendrik R. A. Jonker, Krishna Saxena, Denis Kudlinzki, Verena Linhard, Sridhar Sreeramulu, Andreas Heine, Harald Schwalbe, Gerhard Klebe
Pubblicato in: ChemMedChem, Numero 13/18, 2018, Pagina/e 1988-1996, ISSN 1860-7179
Editore: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/cmdc.201800438

Structure of Type-I Mycobacterium tuberculosis fatty acid synthase at 3.3 Å resolution

Autori: Nadav Elad, Szilvia Baron, Yoav Peleg, Shira Albeck, Jacob Grunwald, Gal Raviv, Zippora Shakked, Oren Zimhony, Ron Diskin
Pubblicato in: Nature Communications, Numero 9/1, 2018, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-018-06440-6

Homology-based loop modeling yields more complete crystallographic protein structures

Autori: Bart van Beusekom, Krista Joosten, Maarten L. Hekkelman, Robbie P. Joosten, Anastassis Perrakis
Pubblicato in: IUCrJ, Numero 5/5, 2018, Pagina/e 585-594, ISSN 2052-2525
Editore: IUCr
DOI: 10.1107/s2052252518010552

Comment on “Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water”

Autori: Gustavo Fuertes, Niccolo Banterle, Kiersten M. Ruff, Aritra Chowdhury, Rohit V. Pappu, Dmitri I. Svergun, Edward A. Lemke
Pubblicato in: Science, Numero 361/6405, 2018, Pagina/e eaau8230, ISSN 0036-8075
Editore: American Association for the Advancement of Science
DOI: 10.1126/science.aau8230

Tripeptide binding in a proton-dependent oligopeptide transporter

Autori: Maria Martinez Molledo, Esben M. Quistgaard, Christian Löw
Pubblicato in: FEBS Letters, Numero 592/19, 2018, Pagina/e 3239-3247, ISSN 0014-5793
Editore: Elsevier BV
DOI: 10.1002/1873-3468.13246

Structural and biochemical characterization of the Cutibacterium acnes exo-β-1,4-mannosidase that targets the N-glycan core of host glycoproteins

Autori: Tom Reichenbach, Dayanand Kalyani, Rosaria Gandini, Olov Svartström, Henrik Aspeborg, Christina Divne
Pubblicato in: PLOS ONE, Numero 13/9, 2018, Pagina/e e0204703, ISSN 1932-6203
Editore: Public Library of Science
DOI: 10.1371/journal.pone.0204703

Multivariate Control of Transcript to Protein Variability in Single Mammalian Cells

Autori: Doris Popovic, Birgit Koch, Moritz Kueblbeck, Jan Ellenberg, Lucas Pelkmans
Pubblicato in: Cell Systems, Numero 7/4, 2018, Pagina/e 398-411.e6, ISSN 2405-4712
Editore: Elsevier
DOI: 10.1016/j.cels.2018.09.001

Cryo-EM structures of human STEAP4 reveal mechanism of iron(III) reduction

Autori: Wout Oosterheert, Laura S. van Bezouwen, Remco N. P. Rodenburg, Joke Granneman, Friedrich Förster, Andrea Mattevi, Piet Gros
Pubblicato in: Nature Communications, Numero 9/1, 2018, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-018-06817-7

Subtomogram averaging of COPII assemblies reveals how coat organization dictates membrane shape

Autori: Joshua Hutchings, Viktoriya Stancheva, Elizabeth A. Miller, Giulia Zanetti
Pubblicato in: Nature Communications, Numero 9/1, 2018, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-018-06577-4

Molecular Recognition of a Thomsen-Friedenreich Antigen Mimetic Targeting Human Galectin-3

Autori: Sabrina Santarsia, Ana Sofia Grosso, Filipa Trovão, Jesús Jiménez-Barbero, Ana Luísa Carvalho, Cristina Nativi, Filipa Marcelo
Pubblicato in: ChemMedChem, Numero 13/19, 2018, Pagina/e 2030-2036, ISSN 1860-7179
Editore: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/cmdc.201800525

The first single particle analysis Map Challenge: A summary of the assessments

Autori: J. Bernard Heymann, Roberto Marabini, Mohsen Kazemi, Carlos Oscar S. Sorzano, Maya Holmdahl, Joshua H. Mendez, Scott M. Stagg, Slavica Jonic, Eugene Palovcak, Jean-Paul Armache, Jianhua Zhao, Yifan Cheng, Grigore Pintilie, Wah Chiu, Ardan Patwardhan, Jose-Maria Carazo
Pubblicato in: Journal of Structural Biology, Numero 204/2, 2018, Pagina/e 291-300, ISSN 1047-8477
Editore: Academic Press
DOI: 10.1016/j.jsb.2018.08.010

Recombinant production of A1S_0222 from Acinetobacter baumannii ATCC 17978 and confirmation of its DNA-(adenine N6)-methyltransferase activity

Autori: Ulrike Blaschke, Beneditta Suwono, Sachli Zafari, Ingo Ebersberger, Evelyn Skiebe, Cy M. Jeffries, Dmitri I. Svergun, Gottfried Wilharm
Pubblicato in: Protein Expression and Purification, Numero 151, 2018, Pagina/e 78-85, ISSN 1046-5928
Editore: Academic Press
DOI: 10.1016/j.pep.2018.06.009

IBA57 Recruits ISCA2 to Form a [2Fe-2S] Cluster-Mediated Complex

Autori: Spyridon Gourdoupis, Veronica Nasta, Vito Calderone, Simone Ciofi-Baffoni, Lucia Banci
Pubblicato in: Journal of the American Chemical Society, Numero 140/43, 2018, Pagina/e 14401-14412, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/jacs.8b09061

DNA recognition by Arabidopsis transcription factors ABI3 and NGA1

Autori: Giedrius Sasnauskas, Elena Manakova, Kęstutis Lapėnas, Kotryna Kauneckaitė, Virginijus Siksnys
Pubblicato in: The FEBS Journal, Numero 285/21, 2018, Pagina/e 4041-4059, ISSN 1742-464X
Editore: Blackwell Publishing Inc.
DOI: 10.1111/febs.14649

Probing the Architecture of a Multi-PDZ Domain Protein: Structure of PDZK1 in Solution

Autori: Hajizadeh NR, Pieprzyk J, Skopintsev P, Flayhan A, Svergun DI, Löw C
Pubblicato in: Structure, Numero 26/11, 2018, Pagina/e 1522-1533.e5, ISSN 0969-2126
Editore: Elsevier
DOI: 10.1016/j.str.2018.07.016

Structure of Mutant Hepatitis B Core Protein Capsids with Premature Secretion Phenotype

Autori: Bettina Böttcher, Michael Nassal
Pubblicato in: Journal of Molecular Biology, Numero 430/24, 2018, Pagina/e 4941-4954, ISSN 0022-2836
Editore: Academic Press
DOI: 10.1016/j.jmb.2018.10.018

Map challenge: Analysis using a pair comparison method based on Fourier shell correlation

Autori: R. Marabini, M. Kazemi, C.O.S. Sorzano, J.M. Carazo
Pubblicato in: Journal of Structural Biology, Numero 204/3, 2018, Pagina/e 527-542, ISSN 1047-8477
Editore: Academic Press
DOI: 10.1016/j.jsb.2018.09.009

A tumor-targeted trimeric 4-1BB-agonistic antibody induces potent anti-tumor immunity without systemic toxicity

Autori: Marta Compte, Seandean Lykke Harwood, Ines G. Muñoz, Rocio Navarro, Manuela Zonca, Gema Perez-Chacon, Ainhoa Erce-Llamazares, Nekane Merino, Antonio Tapia-Galisteo, Angel M. Cuesta, Kasper Mikkelsen, Eduardo Caleiras, Natalia Nuñez-Prado, M. Angela Aznar, Simon Lykkemark, Jorge Martínez-Torrecuadrada, Ignacio Melero, Francisco J. Blanco, Jorge Bernardino de la Serna, Juan M. Zapata, Laura Sanz,
Pubblicato in: Nature Communications, Numero 9/1, 2018, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-018-07195-w

Function and Interactions of ERCC1-XPF in DNA Damage Response

Autori: Maryam Faridounnia, Gert Folkers, Rolf Boelens
Pubblicato in: Molecules, Numero 23/12, 2018, Pagina/e 3205, ISSN 1420-3049
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules23123205

Assessing protein conformational landscapes: integration of DEER data in Maximum Occurrence analysis

Autori: Lucia Gigli, Witold Andrałojć, Arina Dalaloyan, Giacomo Parigi, Enrico Ravera, Daniella Goldfarb, Claudio Luchinat
Pubblicato in: Physical Chemistry Chemical Physics, Numero 20/43, 2018, Pagina/e 27429-27438, ISSN 1463-9076
Editore: Royal Society of Chemistry
DOI: 10.1039/c8cp06195e

Local and Global Dynamics in Intrinsically Disordered Synuclein

Autori: Nasrollah Rezaei-Ghaleh, Giacomo Parigi, Andrea Soranno, Andrea Holla, Stefan Becker, Benjamin Schuler, Claudio Luchinat, Markus Zweckstetter
Pubblicato in: Angewandte Chemie International Edition, Numero 57/46, 2018, Pagina/e 15262-15266, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201808172

Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR

Autori: Wing Ying Chow, Rui Li, Ieva Goldberga, David G. Reid, Rakesh Rajan, Jonathan Clark, Hartmut Oschkinat, Melinda J. Duer, Robert Hayward, Catherine M. Shanahan
Pubblicato in: Chemical Communications, Numero 54/89, 2018, Pagina/e 12570-12573, ISSN 1359-7345
Editore: Royal Society of Chemistry
DOI: 10.1039/c8cc04960b

Using Scipion for stream image processing at Cryo-EM facilities

Autori: J. Gómez-Blanco, J.M. de la Rosa-Trevín, R. Marabini, L. del Cano, A. Jiménez, M. Martínez, R. Melero, T. Majtner, D. Maluenda, J. Mota, Y. Rancel, E Ramírez-Aportela, J.L. Vilas, M. Carroni, S. Fleischmann, E. Lindahl, A.W. Ashton, M. Basham, D.K. Clare, K. Savage, C.A. Siebert, G.G. Sharov, C.O.S. Sorzano, P. Conesa, J.M. Carazo
Pubblicato in: Journal of Structural Biology, Numero 204/3, 2018, Pagina/e 457-463, ISSN 1047-8477
Editore: Academic Press
DOI: 10.1016/j.jsb.2018.10.001

Allosteric Inhibition of a Mammalian Lectin

Autori: Jonas Aretz, Upendra R. Anumala, Felix F. Fuchsberger, Narges Molavi, Nandor Ziebart, Hengxi Zhang, Marc Nazaré, Christoph Rademacher
Pubblicato in: Journal of the American Chemical Society, Numero 140/44, 2018, Pagina/e 14915-14925, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/jacs.8b08644

β 2 -Type Amyloidlike Fibrils of Poly- l -glutamic Acid Convert into Long, Highly Ordered Helices upon Dissolution in Dimethyl Sulfoxide

Autori: Sylwia Berbeć, Robert Dec, Dmitry Molodenskiy, Beata Wielgus-Kutrowska, Christian Johannessen, Agnieszka Hernik-Magoń, Fernando Tobias, Agnieszka Bzowska, Grzegorz Ścibisz, Timothy A. Keiderling, Dmitri Svergun, Wojciech Dzwolak
Pubblicato in: The Journal of Physical Chemistry B, Numero 122/50, 2018, Pagina/e 11895-11905, ISSN 1520-6106
Editore: American Chemical Society
DOI: 10.1021/acs.jpcb.8b08308

Biochemical and structural studies reveal differences and commonalities among cap-snatching endonucleases from segmented negative-strand RNA viruses

Autori: Tobias Holm, Janine-Denise Kopicki, Carola Busch, Silke Olschewski, Maria Rosenthal, Charlotte Uetrecht, Stephan Günther, Sophia Reindl
Pubblicato in: Journal of Biological Chemistry, Numero 293/51, 2018, Pagina/e 19686-19698, ISSN 0021-9258
Editore: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.ra118.004373

Protein Glycosylation through Sulfur Fluoride Exchange (SuFEx) Chemistry: The Key Role of a Fluorosulfate Thiolactoside

Autori: Alberto Marra, Jiajia Dong, Tiancheng Ma, Stefano Giuntini, Elisa Crescenzo, Linda Cerofolini, Marco Martinucci, Claudio Luchinat, Marco Fragai, Cristina Nativi, Alessandro Dondoni
Pubblicato in: Chemistry - A European Journal, Numero 24/71, 2018, Pagina/e 18981-18987, ISSN 0947-6539
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/chem.201803912

Effects of Boron Supplementation on Peripartum Dairy Cows' Health.

Autori: Abdullah Basoglu, Nuri Baspinar, Leonardo Tenori, Alessia Vignoli, Erdem Gulersoy
Pubblicato in: Biological Trace Element Research, 2017, ISSN 0163-4984
Editore: Humana Press, Inc.
DOI: 10.1007/s12011-017-0971-9

Direct assessment of substrate binding to the Neurotransmitter:Sodium Symporter LeuT by solid state NMR

Autori: Simon Erlendsson, Kamil Gotfryd, Flemming Hofmann, Larsen Jonas, Sigurd Mortensen, Michel-Andreas Geiger, Barth-Jan van Rossum, Hartmut Oschkinat, Ulrik Gether, Kaare Teilum, Claus J Loland
Pubblicato in: eLife, 2017, ISSN 2050-084X
Editore: eLife Sciences Publications
DOI: 10.7554/eLife.19314.001

Cryo-EM structure of the spinach chloroplast ribosome reveals the location of plastid-specific ribosomal proteins and extensions

Autori: Michael Graf, Stefan Arenz, Paul Huter, Alexandra Dönhöfer, Jiří Nováček, Daniel N. Wilson
Pubblicato in: Nucleic Acids Research, 2017, Pagina/e gkw1272, ISSN 0305-1048
Editore: Oxford University Press
DOI: 10.1093/nar/gkw1272

Structural Mechanism for Cargo Recognition by the Retromer Complex

Autori: Marıa Lucas, David C. Gershlick, Ander Vidaurrazaga, Adriana L. Rojas, Juan S. Bonifacino, Aitor Hierro
Pubblicato in: Cell, 2016, ISSN 0092-8674
Editore: Cell Press
DOI: 10.1016/j.cell.2016.10.056

Structure determination of a major facilitator peptide transporter: Inward facing PepTSt from Streptococcus thermophilus crystallized in space group P3121

Autori: Esben M. Quistgaard, Maria Martinez Molledo, Christian Löw
Pubblicato in: PLOS one, 2017, ISSN 1932-6203
Editore: Public Library of Science
DOI: 10.1371/journal.pone.0173126

[4Fe-4S] Cluster Assembly in Mitochondria and Its Impairment by Copper

Autori: Diego Brancaccio, Angelo Gallo, Mario Piccioli, Ettore Novellino, Simone Ciofi-Baffoni, Lucia Banci
Pubblicato in: Journal of the American Chemical Society, 2016, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/jacs.6b09567

Gelified Biofluids for High-Resolution Magic Angle Spinning 1H NMR Analysis: The Case of Urine

Autori: Panteleimon G. Takis, Leonardo Tenori, Enrico Ravera, Claudio Luchinat
Pubblicato in: Analytical Chemistry, 2017, ISSN 0003-2700
Editore: American Chemical Society
DOI: 10.1021/acs.analchem.6b04318

Three-Dimensional Structure of Full-Length NtrX, an Unusual Member of the NtrC Family of Response Regulators

Autori: Ignacio Fernández, Irina Cornaciu, Mariela del Carmen Carrica, Emiko Uchikawa, Guillaume Hoffmann, Rodrigo Sieira, José Antonio Márquez, Fernando A. Goldbaum
Pubblicato in: Journal of Molecular Biology, 2017, ISSN 0022-2836
Editore: Academic Press
DOI: 10.1016/j.jmb.2016.12.022

Decision-Making in Cascade Complexes Harboring crRNAs of Altered Length

Autori: Inga Songailiene, Marius Rutkauskas, Tomas Sinkunas, Elena Manakova, Sabine Wittig, Carla Schmidt, Virginijus Siksnys, Ralf Seidel
Pubblicato in: Cell Reports, Numero 28/12, 2019, Pagina/e 3157-3166.e4, ISSN 2211-1247
Editore: Cell Press
DOI: 10.1016/j.celrep.2019.08.033

Engineered Carbonic Anhydrase VI-Mimic Enzyme Switched the Structure and Affinities of Inhibitors

Autori: Justina Kazokaitė, Visvaldas Kairys, Joana Smirnovienė, Alexey Smirnov, Elena Manakova, Martti Tolvanen, Seppo Parkkila, Daumantas Matulis
Pubblicato in: Scientific Reports, Numero 9/1, 2019, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-019-49094-0

Cryo-EM structure of pleconaril-resistant rhinovirus-B5 complexed to the antiviral OBR-5-340 reveals unexpected binding site

Autori: Jiri Wald, Marion Pasin, Martina Richter, Christin Walther, Neann Mathai, Johannes Kirchmair, Vadim A. Makarov, Nikolaus Goessweiner-Mohr, Thomas C. Marlovits, Irene Zanella, Antonio Real-Hohn, Nuria Verdaguer, Dieter Blaas, Michaela Schmidtke
Pubblicato in: Proceedings of the National Academy of Sciences, Numero 116/38, 2019, Pagina/e 19109-19115, ISSN 0027-8424
Editore: National Academy of Sciences
DOI: 10.1073/pnas.1904732116

What are the methodological and theoretical prospects for paramagnetic NMR in structural biology? A glimpse into the crystal ball

Autori: Enrico Ravera, Giacomo Parigi, Claudio Luchinat
Pubblicato in: Journal of Magnetic Resonance, Numero 306, 2019, Pagina/e 173-179, ISSN 1090-7807
Editore: Academic Press
DOI: 10.1016/j.jmr.2019.07.027

Atomic structure of the Epstein-Barr virus portal

Autori: Cristina Machón, Montserrat Fàbrega-Ferrer, Daming Zhou, Ana Cuervo, José L. Carrascosa, David I. Stuart, Miquel Coll
Pubblicato in: Nature Communications, Numero 10/1, 2019, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-019-11706-8

Monitoring DNA–Ligand Interactions in Living Human Cells Using NMR Spectroscopy

Autori: Michaela Krafcikova, Simon Dzatko, Coralie Caron, Anton Granzhan, Radovan Fiala, Tomas Loja, Marie-Paule Teulade-Fichou, Tomas Fessl, Robert Hänsel-Hertsch, Jean-Louis Mergny, Silvie Foldynova-Trantirkova, Lukas Trantirek
Pubblicato in: Journal of the American Chemical Society, Numero 141/34, 2019, Pagina/e 13281-13285, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/jacs.9b03031

Crystal structures of cholera toxin in complex with fucosylated receptors point to importance of secondary binding site

Autori: Joel B. Heim, Vesna Hodnik, Julie E. Heggelund, Gregor Anderluh, Ute Krengel
Pubblicato in: Scientific Reports, Numero 9/1, 2019, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-019-48579-2

Detection of nucleic acids and other low abundance components in native bone and osteosarcoma extracellular matrix by isotope enrichment and DNP-enhanced NMR

Autori: Ieva Goldberga, Rui Li, Wing Ying Chow, David G. Reid, Ulyana Bashtanova, Rakesh Rajan, Anna Puszkarska, Hartmut Oschkinat, Melinda J. Duer
Pubblicato in: RSC Advances, Numero 9/46, 2019, Pagina/e 26686-26690, ISSN 2046-2069
Editore: Royal Society of Chemistry
DOI: 10.1039/c9ra03198g

Molecular determinants of the mechanism and substrate specificity of Clostridium difficile proline-proline endopeptidase-1

Autori: Christian Pichlo, Linda Juetten, Fabian Wojtalla, Magdalena Schacherl, Dolores Diaz, Ulrich Baumann
Pubblicato in: Journal of Biological Chemistry, Numero 294/30, 2019, Pagina/e 11525-11535, ISSN 0021-9258
Editore: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.ra119.009029

Structures of T7 bacteriophage portal and tail suggest a viral DNA retention and ejection mechanism

Autori: Ana Cuervo, Montserrat Fàbrega-Ferrer, Cristina Machón, José Javier Conesa, Francisco J. Fernández, Rosa Pérez-Luque, Mar Pérez-Ruiz, Joan Pous, M. Cristina Vega, José L. Carrascosa, Miquel Coll
Pubblicato in: Nature Communications, Numero 10/1, 2019, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-019-11705-9

Validation of electron microscopy initial models via small angle X-ray scattering curves

Autori: Amaya Jiménez, Slavica Jonic, Tomas Majtner, Joaquín Otón, Jose Luis Vilas, David Maluenda, Javier Mota, Erney Ramírez-Aportela, Marta Martínez, Yaiza Rancel, Joan Segura, Ruben Sánchez-García, Roberto Melero, Laura del Cano, Pablo Conesa, Lars Skjaerven, Roberto Marabini, Jose M Carazo, Carlos Oscar S Sorzano
Pubblicato in: Bioinformatics, Numero 35/14, 2018, Pagina/e 2427-2433, ISSN 1367-4803
Editore: Oxford University Press
DOI: 10.1093/bioinformatics/bty985

Integrative Approaches in Structural Biology: A More Complete Picture from the Combination of Individual Techniques

Autori: Linda Cerofolini, Marco Fragai, Enrico Ravera, Christoph A. Diebolder, Ludovic Renault, Vito Calderone
Pubblicato in: Biomolecules, Numero 9/8, 2019, Pagina/e 370, ISSN 2218-273X
Editore: -
DOI: 10.3390/biom9080370

Structural Basis of Transcription: RNA Polymerase Backtracking and Its Reactivation

Autori: Mo’men Abdelkareem, Charlotte Saint-André, Maria Takacs, Gabor Papai, Corinne Crucifix, Xieyang Guo, Julio Ortiz, Albert Weixlbaumer
Pubblicato in: Molecular Cell, Numero 75/2, 2019, Pagina/e 298-309.e4, ISSN 1097-2765
Editore: Cell Press
DOI: 10.1016/j.molcel.2019.04.029

Molecular tuning of farnesoid X receptor partial agonism

Autori: Daniel Merk, Sridhar Sreeramulu, Denis Kudlinzki, Krishna Saxena, Verena Linhard, Santosh L. Gande, Fabian Hiller, Christina Lamers, Ewa Nilsson, Anna Aagaard, Lisa Wissler, Niek Dekker, Krister Bamberg, Manfred Schubert-Zsilavecz, Harald Schwalbe
Pubblicato in: Nature Communications, Numero 10/1, 2019, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-019-10853-2

High-throughput stability screening for detergent-solubilized membrane proteins

Autori: Vadim Kotov, Kim Bartels, Katharina Veith, Inokentijs Josts, Udaya K. Tiruttani Subhramanyam, Christian Günther, Jörg Labahn, Thomas C. Marlovits, Isabel Moraes, Henning Tidow, Christian Löw, Maria M. Garcia-Alai
Pubblicato in: Scientific Reports, Numero 9/1, 2019, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-019-46686-8

DNP-Supported Solid-State NMR Spectroscopy of Proteins Inside Mammalian Cells

Autori: Siddarth Narasimhan, Stephan Scherpe, Alessandra Lucini Paioni, Johan van der Zwan, Gert E. Folkers, Huib Ovaa, Marc Baldus
Pubblicato in: Angewandte Chemie International Edition, Numero 58/37, 2019, Pagina/e 12969-12973, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201903246

Joint X-ray/NMR structure refinement of multidomain/multisubunit systems

Autori: Azzurra Carlon, Enrico Ravera, Giacomo Parigi, Garib N. Murshudov, Claudio Luchinat
Pubblicato in: Journal of Biomolecular NMR, Numero 73/6-7, 2019, Pagina/e 265-278, ISSN 0925-2738
Editore: Kluwer Academic Publishers
DOI: 10.1007/s10858-018-0212-3

High-throughput peptide-MHC complex generation and kinetic screenings of TCRs with peptide-receptive HLA-A*02:01 molecules

Autori: Andreas Moritz, Raghavendra Anjanappa, Claudia Wagner, Sebastian Bunk, Martin Hofmann, Gabriele Pszolla, Ankur Saikia, Maria Garcia-Alai, Rob Meijers, Hans-Georg Rammensee, Sebastian Springer, Dominik Maurer
Pubblicato in: Science Immunology, Numero 4/37, 2019, Pagina/e eaav0860, ISSN 2470-9468
Editore: -
DOI: 10.1126/sciimmunol.aav0860

Empty peptide-receptive MHC class I molecules for efficient detection of antigen-specific T cells

Autori: Sunil Kumar Saini, Tripti Tamhane, Raghavendra Anjanappa, Ankur Saikia, Sofie Ramskov, Marco Donia, Inge Marie Svane, Søren Nyboe Jakobsen, Maria Garcia-Alai, Martin Zacharias, Rob Meijers, Sebastian Springer, Sine Reker Hadrup
Pubblicato in: Science Immunology, Numero 4/37, 2019, Pagina/e eaau9039, ISSN 2470-9468
Editore: -
DOI: 10.1126/sciimmunol.aau9039

Absolute quantification of cohesin, CTCF and their regulators in human cells

Autori: Johann Holzmann, Antonio Z Politi, Kota Nagasaka, Merle Hantsche-Grininger, Nike Walther, Birgit Koch, Johannes Fuchs, Gerhard Dürnberger, Wen Tang, Rene Ladurner, Roman R Stocsits, Georg A Busslinger, Béla Novák, Karl Mechtler, Iain Finley Davidson, Jan Ellenberg, Jan-Michael Peters
Pubblicato in: eLife, Numero 8, 2019, ISSN 2050-084X
Editore: eLife Sciences Publications
DOI: 10.7554/elife.46269

A novel druggable interprotomer pocket in the capsid of rhino- and enteroviruses

Autori: Rana Abdelnabi, James A. Geraets, Yipeng Ma, Carmen Mirabelli, Justin W. Flatt, Aušra Domanska, Leen Delang, Dirk Jochmans, Timiri Ajay Kumar, Venkatesan Jayaprakash, Barij Nayan Sinha, Pieter Leyssen, Sarah J. Butcher, Johan Neyts
Pubblicato in: PLOS Biology, Numero 17/6, 2019, Pagina/e e3000281, ISSN 1545-7885
Editore: -
DOI: 10.1371/journal.pbio.3000281

Determining cellular CTCF and cohesin abundances to constrain 3D genome models

Autori: Claudia Cattoglio, Iryna Pustova, Nike Walther, Jaclyn J Ho, Merle Hantsche-Grininger, Carla J Inouye, M Julius Hossain, Gina M Dailey, Jan Ellenberg, Xavier Darzacq, Robert Tjian, Anders S Hansen
Pubblicato in: eLife, Numero 8, 2019, ISSN 2050-084X
Editore: eLife Sciences Publications
DOI: 10.7554/elife.40164

Autori: Arne Raasakka, Salla Ruskamo, Robert Barker, Oda C. Krokengen, Guro H. Vatne, Cecilie K. Kristiansen, Erik I. Hallin, Maximilian W. A. Skoda, Ulrich Bergmann, Hanna Wacklin-Knecht, Nykola C. Jones, Søren V. Hoffmann, Petri Kursula
Pubblicato in: PLOS ONE, Numero 14/6, 2019, Pagina/e e0216833, ISSN 1932-6203
Editore: Public Library of Science
DOI: 10.1371/journal.pone.0216833

An Emerin LEM-Domain Mutation Impairs Cell Response to Mechanical Stress

Autori: Nada Essawy, Camille Samson, Ambre Petitalot, Sophie Moog, Anne Bigot, Isaline Herrada, Agathe Marcelot, Ana-Andreea Arteni, Catherine Coirault, Sophie Zinn-Justin
Pubblicato in: Cells, Numero 8/6, 2019, Pagina/e 570, ISSN 2073-4409
Editore: -
DOI: 10.3390/cells8060570

Protective Dps–DNA co‐crystallization in stressed cells: an in vitro structural study by small‐angle X‐ray scattering and cryo‐electron tomography

Autori: Liubov A. Dadinova, Yurii M. Chesnokov, Roman A. Kamyshinsky, Ivan A. Orlov, Maxim V. Petoukhov, Andrey A. Mozhaev, Ekaterina Yu. Soshinskaya, Vassili N. Lazarev, Valentin A. Manuvera, Anton S. Orekhov, Alexander L. Vasiliev, Eleonora V. Shtykova
Pubblicato in: FEBS Letters, Numero 593/12, 2019, Pagina/e 1360-1371, ISSN 0014-5793
Editore: Elsevier BV
DOI: 10.1002/1873-3468.13439

The structure of a membrane adenylyl cyclase bound to an activated stimulatory G protein

Autori: Chao Qi, Simona Sorrentino, Ohad Medalia, Volodymyr M. Korkhov
Pubblicato in: Science, Numero 364/6438, 2019, Pagina/e 389-394, ISSN 0036-8075
Editore: American Association for the Advancement of Science
DOI: 10.1126/science.aav0778

Side chain to main chain hydrogen bonds stabilize a polyglutamine helix in a transcription factor

Autori: Albert Escobedo, Busra Topal, Micha B. A. Kunze, Juan Aranda, Giulio Chiesa, Daniele Mungianu, Ganeko Bernardo-Seisdedos, Bahareh Eftekharzadeh, Margarida Gairí, Roberta Pierattelli, Isabella C. Felli, Tammo Diercks, Oscar Millet, Jesús García, Modesto Orozco, Ramon Crehuet, Kresten Lindorff-Larsen, Xavier Salvatella
Pubblicato in: Nature Communications, Numero 10/1, 2019, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-019-09923-2

A Tail-Based Mechanism Drives Nucleosome Demethylation by the LSD2/NPAC Multimeric Complex

Autori: Chiara Marabelli, Biagina Marrocco, Simona Pilotto, Sagar Chittori, Sarah Picaud, Sara Marchese, Giuseppe Ciossani, Federico Forneris, Panagis Filippakopoulos, Guy Schoehn, Daniela Rhodes, Sriram Subramaniam, Andrea Mattevi
Pubblicato in: Cell Reports, Numero 27/2, 2019, Pagina/e 387-399.e7, ISSN 2211-1247
Editore: Cell Press
DOI: 10.1016/j.celrep.2019.03.061

Structural characterization of a protein adsorbed on aluminum hydroxide adjuvant in vaccine formulation

Autori: Linda Cerofolini, Stefano Giuntini, Enrico Ravera, Claudio Luchinat, Francesco Berti, Marco Fragai
Pubblicato in: npj Vaccines, Numero 4/1, 2019, ISSN 2059-0105
Editore: -
DOI: 10.1038/s41541-019-0115-7

The carbon monoxide dehydrogenase accessory protein CooJ is a histidine-rich multidomain dimer containing an unexpected Ni(II)-binding site

Autori: Marila Alfano, Julien Pérard, Philippe Carpentier, Christian Basset, Barbara Zambelli, Jennifer Timm, Serge Crouzy, Stefano Ciurli, Christine Cavazza
Pubblicato in: Journal of Biological Chemistry, Numero 294/19, 2019, Pagina/e 7601-7614, ISSN 0021-9258
Editore: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.ra119.008011

Structural Basis for the Action of an All-Purpose Transcription Anti-termination Factor

Autori: Ferdinand Krupp, Nelly Said, Yong-Heng Huang, Bernhard Loll, Jörg Bürger, Thorsten Mielke, Christian M.T. Spahn, Markus C. Wahl
Pubblicato in: Molecular Cell, Numero 74/1, 2019, Pagina/e 143-157.e5, ISSN 1097-2765
Editore: Cell Press
DOI: 10.1016/j.molcel.2019.01.016

High-Resolution Crystal Structure of Endoplasmic Reticulum Aminopeptidase 1 with Bound Phosphinic Transition-State Analogue Inhibitor

Autori: Petros Giastas, Margarete Neu, Paul Rowland, Efstratios Stratikos
Pubblicato in: ACS Medicinal Chemistry Letters, Numero 10/5, 2019, Pagina/e 708-713, ISSN 1948-5875
Editore: American Chemical Society
DOI: 10.1021/acsmedchemlett.9b00002

New insights into the tetrameric family of the Fur metalloregulators

Autori: S. Nader, J. Pérard, P. Carpentier, L. Arnaud, S. Crouzy, I. Michaud-Soret
Pubblicato in: BioMetals, Numero 32/3, 2019, Pagina/e 501-519, ISSN 0966-0844
Editore: Kluwer Academic Publishers
DOI: 10.1007/s10534-019-00201-8

MXCuBE2 : the dawn of MXCuBE Collaboration

Autori: Marcus Oscarsson, Antonia Beteva, David Flot, Elspeth Gordon, Matias Guijarro, Gordon Leonard, Sean McSweeney, Stephanie Monaco, Christoph Mueller-Dieckmann, Max Nanao, Didier Nurizzo, Alexander N. Popov, David von Stetten, Olof Svensson, Vicente Rey-Bakaikoa, Idrissou Chado, Leonard M. G. Chavas, Laurent Gadea, Patrick Gourhant, Tatiana Isabet, Pierre Legrand, Martin Savko, Serena Sirigu, William
Pubblicato in: Journal of Synchrotron Radiation, Numero 26/2, 2019, Pagina/e 393-405, ISSN 1600-5775
Editore: -
DOI: 10.1107/s1600577519001267

Cryo-EM fibril structures from systemic AA amyloidosis reveal the species complementarity of pathological amyloids

Autori: Falk Liberta, Sarah Loerch, Matthies Rennegarbe, Angelika Schierhorn, Per Westermark, Gunilla T. Westermark, Bouke P. C. Hazenberg, Nikolaus Grigorieff, Marcus Fändrich, Matthias Schmidt
Pubblicato in: Nature Communications, Numero 10/1, 2019, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-019-09033-z

Cryo-EM structure of a light chain-derived amyloid fibril from a patient with systemic AL amyloidosis

Autori: Lynn Radamaker, Yin-Hsi Lin, Karthikeyan Annamalai, Stefanie Huhn, Ute Hegenbart, Stefan O. Schönland, Günter Fritz, Matthias Schmidt, Marcus Fändrich
Pubblicato in: Nature Communications, Numero 10/1, 2019, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-019-09032-0

Structure of Prototypic Peptide Transporter DtpA from E. coli in Complex with Valganciclovir Provides Insights into Drug Binding of Human PepT1

Autori: Yonca Ural-Blimke, Ali Flayhan, Jan Strauss, Vasileios Rantos, Kim Bartels, Rolf Nielsen, Els Pardon, Jan Steyaert, Jan Kosinski, Esben M. Quistgaard, Christian Löw
Pubblicato in: Journal of the American Chemical Society, Numero 141/6, 2019, Pagina/e 2404-2412, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/jacs.8b11343

Design, Synthesis and Biological Evaluation of Isoxazole-Based CK1 Inhibitors Modified with Chiral Pyrrolidine Scaffolds

Autori: Andreas Luxenburger, Dorian Schmidt, Chiara Ianes, Christian Pichlo, Marc Krüger, Thorsten von Drathen, Elena Brunstein, Graeme Gainsford, Ulrich Baumann, Uwe Knippschild, Christian Peifer
Pubblicato in: Molecules, Numero 24/5, 2019, Pagina/e 873, ISSN 1420-3049
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules24050873

A post-translational modification of human Norovirus capsid protein attenuates glycan binding

Autori: Alvaro Mallagaray, Robert Creutznacher, Jasmin Dülfer, Philipp H. O. Mayer, Lena Lisbeth Grimm, Jose Maria Orduña, Esben Trabjerg, Thilo Stehle, Kasper D. Rand, Bärbel S. Blaum, Charlotte Uetrecht, Thomas Peters
Pubblicato in: Nature Communications, Numero 10/1, 2019, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-019-09251-5

Impact of lipid binding on the tertiary structure and allergenic potential of Jug r 3, the non-specific lipid transfer protein from walnut

Autori: Pawel Dubiela, Rebecca Del Conte, Francesca Cantini, Tomasz Borowski, Roberta Aina, Christian Radauer, Merima Bublin, Karin Hoffmann-Sommergruber, Stefano Alessandri
Pubblicato in: Scientific Reports, Numero 9/1, 2019, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-019-38563-1

A framework for tracer-based metabolism in mammalian cells by NMR

Autori: Raquel Saborano, Zuhal Eraslan, Jennie Roberts, Farhat L. Khanim, Patricia F. Lalor, Michelle A. C. Reed, Ulrich L. Günther
Pubblicato in: Scientific Reports, Numero 9/1, 2019, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-018-37525-3

Building and rebuilding N-glycans in protein structure models

Autori: Bart van Beusekom, Natasja Wezel, Maarten L. Hekkelman, Anastassis Perrakis, Paul Emsley, Robbie P. Joosten
Pubblicato in: Acta Crystallographica Section D Structural Biology, Numero 75/4, 2019, Pagina/e 416-425, ISSN 2059-7983
Editore: -
DOI: 10.1107/s2059798319003875

Structural basis for assembly of vertical single β-barrel viruses

Autori: Isaac Santos-Pérez, Diego Charro, David Gil-Carton, Mikel Azkargorta, Felix Elortza, Dennis H. Bamford, Hanna M. Oksanen, Nicola G. A. Abrescia
Pubblicato in: Nature Communications, Numero 10/1, 2019, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-019-08927-2

Real-Time Insights into Biological Events: In-Cell Processes and Protein-Ligand Interactions

Autori: Linda Cerofolini, Stefano Giuntini, Letizia Barbieri, Matteo Pennestri, Anna Codina, Marco Fragai, Lucia Banci, Enrico Luchinat, Enrico Ravera
Pubblicato in: Biophysical Journal, Numero 116/2, 2019, Pagina/e 239-247, ISSN 0006-3495
Editore: Biophysical Society
DOI: 10.1016/j.bpj.2018.11.3132

Cadmium effects on superoxide dismutase 1 in human cells revealed by NMR

Autori: Panagis Polykretis, Francesca Cencetti, Chiara Donati, Enrico Luchinat, Lucia Banci
Pubblicato in: Redox Biology, Numero 21, 2019, Pagina/e 101102, ISSN 2213-2317
Editore: Elsevier BV
DOI: 10.1016/j.redox.2019.101102

A 2.8-Angstrom-Resolution Cryo-Electron Microscopy Structure of Human Parechovirus 3 in Complex with Fab from a Neutralizing Antibody

Autori: Aušra Domanska, Justin W. Flatt, Joonas J. J. Jukonen, James A. Geraets, Sarah J. Butcher
Pubblicato in: Journal of Virology, Numero 93/4, 2019, ISSN 0022-538X
Editore: American Society for Microbiology
DOI: 10.1128/jvi.01597-18

Regulation of Human Hsc70 ATPase and Chaperone Activities by Apg2: Role of the Acidic Subdomain

Autori: Yovana Cabrera, Leire Dublang, José Angel Fernández-Higuero, David Albesa-Jové, Maria Lucas, Ana Rosa Viguera, Marcelo E. Guerin, Jose M.G. Vilar, Arturo Muga, Fernando Moro
Pubblicato in: Journal of Molecular Biology, Numero 431/2, 2019, Pagina/e 444-461, ISSN 0022-2836
Editore: Academic Press
DOI: 10.1016/j.jmb.2018.11.026

Utilization of Staphylococcal Immune Evasion Protein Sbi as a Novel Vaccine Adjuvant

Autori: Yi Yang, Catherine R. Back, Melissa A. Gräwert, Ayla A. Wahid, Harriet Denton, Rebecca Kildani, Joshua Paulin, Kristin Wörner, Wolgang Kaiser, Dmitri I. Svergun, Asel Sartbaeva, Andrew G. Watts, Kevin J. Marchbank, Jean M. H. van den Elsen
Pubblicato in: Frontiers in Immunology, Numero 9, 2019, ISSN 1664-3224
Editore: -
DOI: 10.3389/fimmu.2018.03139

Survey of the analysis of continuous conformational variability of biological macromolecules by electron microscopy

Autori: C. O. S. Sorzano, A. Jiménez, J. Mota, J. L. Vilas, D. Maluenda, M. Martínez, E. Ramírez-Aportela, T. Majtner, J. Segura, R. Sánchez-García, Y. Rancel, L. del Caño, P. Conesa, R. Melero, S. Jonic, J. Vargas, F. Cazals, Z. Freyberg, J. Krieger, I. Bahar, R. Marabini, J. M. Carazo
Pubblicato in: Acta Crystallographica Section F Structural Biology Communications, Numero 75/1, 2019, Pagina/e 19-32, ISSN 2053-230X
Editore: John Wiley and Sons Ltd
DOI: 10.1107/s2053230x18015108

Metal cofactors trafficking and assembly in the cell: a molecular view

Autori: Francesca Camponeschi, Lucia Banci
Pubblicato in: Pure and Applied Chemistry, Numero 91/2, 2019, Pagina/e 231-245, ISSN 0033-4545
Editore: International Union of Pure and Applied Chemistry
DOI: 10.1515/pac-2018-0720

Conformational switch in the ribosomal protein S1 guides unfolding of structured RNAs for translation initiation

Autori: Nusrat Shahin Qureshi, Jasleen Kaur Bains, Sridhar Sreeramulu, Harald Schwalbe, Boris Fürtig
Pubblicato in: Nucleic Acids Research, 2018, ISSN 0305-1048
Editore: Oxford University Press
DOI: 10.1093/nar/gky746

Correlative cryo super-resolution light and electron microscopy on mammalian cells using fluorescent proteins

Autori: Maarten W. Tuijtel, Abraham J. Koster, Stefan Jakobs, Frank G. A. Faas, Thomas H. Sharp
Pubblicato in: Scientific Reports, Numero 9/1, 2019, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-018-37728-8

GM130 and p115 play a key role in the organisation of the early secretory pathway during skeletal muscle differentiation

Autori: Emiliana Giacomello, Paolo Ronchi, Rainer Pepperkok
Pubblicato in: Journal of Cell Science, Numero 132/2, 2019, Pagina/e jcs222083, ISSN 0021-9533
Editore: The Company of Biologists Ltd.
DOI: 10.1242/jcs.222083

Shedding Light on the Interaction of Human Anti-Apoptotic Bcl-2 Protein with Ligands through Biophysical and in Silico Studies

Autori: Joao Ramos, Jayaraman Muthukumaran, Filipe Freire, João Paquete-Ferreira, Ana Otrelo-Cardoso, Dmitri Svergun, Alejandro Panjkovich, Teresa Santos-Silva
Pubblicato in: International Journal of Molecular Sciences, Numero 20/4, 2019, Pagina/e 860, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms20040860

NMR Characterization of Long‐Range Contacts in Intrinsically Disordered Proteins from Paramagnetic Relaxation Enhancement in 13 C Direct‐Detection Experiments

Autori: Borja Mateos, Robert Konrat, Roberta Pierattelli, Isabella C. Felli
Pubblicato in: ChemBioChem, Numero 20/3, 2018, Pagina/e 335-339, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201800539

3DBIONOTES v3.0: crossing molecular and structural biology data with genomic variations

Autori: Joan Segura, Ruben Sanchez-Garcia, C O S Sorzano, J M Carazo
Pubblicato in: Bioinformatics, Numero 35/18, 2019, Pagina/e 3512-3513, ISSN 1367-4803
Editore: Oxford University Press
DOI: 10.1093/bioinformatics/btz118

DeepRes : a new deep-learning- and aspect-based local resolution method for electron-microscopy maps

Autori: Erney Ramírez-Aportela, Javier Mota, Pablo Conesa, Jose Maria Carazo, Carlos Oscar S. Sorzano
Pubblicato in: IUCrJ, Numero 6/6, 2019, ISSN 2052-2525
Editore: -
DOI: 10.1107/s2052252519011692

Automatic local resolution-based sharpening of cryo-EM maps

Autori: Erney Ramírez-Aportela, Jose Luis Vilas, Alisa Glukhova, Roberto Melero, Pablo Conesa, Marta Martínez, David Maluenda, Javier Mota, Amaya Jiménez, Javier Vargas, Roberto Marabini, Patrick M Sexton, Jose Maria Carazo, Carlos Oscar S Sorzano
Pubblicato in: Bioinformatics, 2019, ISSN 1367-4803
Editore: Oxford University Press
DOI: 10.1093/bioinformatics/btz671

Advances in image processing for single-particle analysis by electron cryomicroscopy and challenges ahead

Autori: J.L. Vilas, N. Tabassum, J. Mota, D. Maluenda, A. Jiménez-Moreno, T. Majtner, J.M. Carazo, S.T. Acton, C.O.S. Sorzano
Pubblicato in: Current Opinion in Structural Biology, Numero 52, 2018, Pagina/e 127-145, ISSN 0959-440X
Editore: Elsevier BV
DOI: 10.1016/j.sbi.2018.11.004

Correction: Structural plasticity of the N-terminal capping helix of the TPR domain of kinesin light chain

Autori: The Quyen Nguyen, Mélanie Chenon, Fernando Vilela, Christophe Velours, Magali Aumont-Nicaise, Jessica Andreani, Paloma F. Varela, Paola Llinas, Julie Ménétrey
Pubblicato in: PLOS ONE, Numero 13/5, 2018, Pagina/e e0197193, ISSN 1932-6203
Editore: Public Library of Science
DOI: 10.1371/journal.pone.0197193

Advances in cryo-electron tomography for biology and medicine

Autori: Roman I. Koning, Abraham J. Koster, Thomas H. Sharp
Pubblicato in: Annals of Anatomy - Anatomischer Anzeiger, Numero 217, 2018, Pagina/e 82-96, ISSN 0940-9602
Editore: Elsevier BV
DOI: 10.1016/j.aanat.2018.02.004

NMR Study of Rcf2 Reveals an Unusual Dimeric Topology in Detergent Micelles

Autori: Shu Zhou, Pontus Pettersson, Peter Brzezinski, Pia Ädelroth, Lena Mäler
Pubblicato in: ChemBioChem, Numero 19/5, 2018, Pagina/e 444-447, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201700664

XTEND: Extending the depth of field in cryo soft X-ray tomography

Autori: Joaquín Otón, Eva Pereiro, José J. Conesa, Francisco J. Chichón, Daniel Luque, Javier M. Rodríguez, Ana J. Pérez-Berná, Carlos Oscar S. Sorzano, Joanna Klukowska, Gabor T. Herman, Javier Vargas, Roberto Marabini, José L. Carrascosa, José M. Carazo
Pubblicato in: Scientific Reports, Numero 7, 2017, Pagina/e 45808, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/srep45808

Solution NMR Structure of a Ligand/Hybrid-2-G-Quadruplex Complex Reveals Rearrangements that Affect Ligand Binding

Autori: Julia Wirmer-Bartoschek, Lars Erik Bendel, Hendrik R. A. Jonker, J. Tassilo Grün, Francesco Papi, Carla Bazzicalupi, Luigi Messori, Paola Gratteri, Harald Schwalbe
Pubblicato in: Angewandte Chemie International Edition, 2017, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201702135

ATSAS 2.8 : a comprehensive data analysis suite for small-angle scattering from macromolecular solutions

Autori: D. Franke, M. V. Petoukhov, P. V. Konarev, A. Panjkovich, A. Tuukkanen, H. D. T. Mertens, A. G. Kikhney, N. R. Hajizadeh, J. M. Franklin, C. M. Jeffries, D. I. Svergun
Pubblicato in: Journal of Applied Crystallography, Numero 50/4, 2017, ISSN 1600-5767
Editore: IUCr/Wiley
DOI: 10.1107/S1600576717007786

Crabrolin, a natural antimicrobial peptide: structural properties

Autori: Massimiliano Aschi, Argante Bozzi, Carla Luzi, Nadia Bouchemal, Marco Sette
Pubblicato in: Journal of Peptide Science, Numero June 5, 2017, ISSN 1075-2617
Editore: John Wiley & Sons Inc.
DOI: 10.1002/psc.3013

Mistakes in translation: Reflections on mechanism

Autori: Yizhou Liu, Joshua S. Sharp, Duc H-T. Do, Richard A. Kahn, Harald Schwalbe, Florian Buhr, James H. Prestegard
Pubblicato in: PLOS ONE, Numero 12/6, 2017, Pagina/e e0180566, ISSN 1932-6203
Editore: Public Library of Science
DOI: 10.1371/journal.pone.0180566

Structural insights into the molecular function of human [2Fe-2S] BOLA1-GRX5 and [2Fe-2S] BOLA3-GRX5 complexes

Autori: Veronica Nasta, Andrea Giachetti, Simone Ciofi-Baffoni, Lucia Banci
Pubblicato in: Biochimica et Biophysica Acta (BBA) - General Subjects, Numero 1861/8, 2017, Pagina/e 2119-2131, ISSN 0304-4165
Editore: Elsevier BV
DOI: 10.1016/j.bbagen.2017.05.005

A Spring-Loaded Mechanism Governs the Clamp-like Dynamics of the Skp Chaperone

Autori: Daniel A. Holdbrook, Björn M. Burmann, Roland G. Huber, Maxim V. Petoukhov, Dmitri I. Svergun, Sebastian Hiller, Peter J. Bond
Pubblicato in: Structure, Numero 25/7, 2017, Pagina/e 1079-1088.e3, ISSN 0969-2126
Editore: Cell Press
DOI: 10.1016/j.str.2017.05.018

Paramagnetic Properties of a Crystalline Iron–Sulfur Protein by Magic-Angle Spinning NMR Spectroscopy

Autori: Andrea Bertarello, Tobias Schubeis, Carmelo Fuccio, Enrico Ravera, Marco Fragai, Giacomo Parigi, Lyndon Emsley, Guido Pintacuda, Claudio Luchinat
Pubblicato in: Inorganic Chemistry, Numero 56/11, 2017, Pagina/e 6624-6629, ISSN 0020-1669
Editore: American Chemical Society
DOI: 10.1021/acs.inorgchem.7b00674

Combining NMR and small angle X-ray scattering for the study of biomolecular structure and dynamics

Autori: Haydyn D.T. Mertens, Dmitri I. Svergun
Pubblicato in: Archives of Biochemistry and Biophysics, Numero 628, 2017, Pagina/e 33-41, ISSN 0003-9861
Editore: Academic Press
DOI: 10.1016/j.abb.2017.05.005

Anthranilate phosphoribosyltransferase from the hyperthermophilic archaeon Thermococcus kodakarensis shows maximum activity with zinc and forms a unique dimeric structure

Autori: Sumera Perveen, Naeem Rashid, Xiao-Feng Tang, Tadayuki Imanaka, Anastassios C. Papageorgiou
Pubblicato in: FEBS Open Bio, Numero 7/8, 2017, Pagina/e 1217-1230, ISSN 2211-5463
Editore: Elsevier BV
DOI: 10.1002/2211-5463.12264

A Novel Approach to Data Collection for Difficult Structures: Data Management for Large Numbers of Crystals with the BLEND Software

Autori: Anastasia Mylona, Stephen Carr, Pierre Aller, Isabel Moraes, Richard Treisman, Gwyndaf Evans, James Foadi
Pubblicato in: Crystals, Numero 7/8, 2017, Pagina/e 242, ISSN 2073-4352
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cryst7080242

Anamorsin/Ndor1 Complex Reduces [2Fe–2S]-MitoNEET via a Transient Protein–Protein Interaction

Autori: Francesca Camponeschi, Simone Ciofi-Baffoni, Lucia Banci
Pubblicato in: Journal of the American Chemical Society, Numero 139/28, 2017, Pagina/e 9479-9482, ISSN 0002-7863
Editore: American Chemical Society
DOI: 10.1021/jacs.7b05003

An antimicrobial peptide that inhibits translation by trapping release factors on the ribosome

Autori: Tanja Florin, Cristina Maracci, Michael Graf, Prajwal Karki, Dorota Klepacki, Otto Berninghausen, Roland Beckmann, Nora Vázquez-Laslop, Daniel N Wilson, Marina V Rodnina, Alexander S Mankin
Pubblicato in: Nature Structural & Molecular Biology, Numero 24/9, 2017, Pagina/e 752-757, ISSN 1545-9993
Editore: Nature Publishing Group
DOI: 10.1038/nsmb.3439

Lipid-like Peptides can Stabilize Integral Membrane Proteins for Biophysical and Structural Studies

Autori: Katharina Veith, Maria Martinez Molledo, Yasser Almeida Hernandez, Inokentijs Josts, Julius Nitsche, Christian Löw, Henning Tidow
Pubblicato in: ChemBioChem, Numero 18/17, 2017, Pagina/e 1735-1742, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201700235

Evaluation of 15N-detected H–N correlation experiments on increasingly large RNAs

Autori: Robbin Schnieders, Christian Richter, Sven Warhaut, Vanessa de Jesus, Sara Keyhani, Elke Duchardt-Ferner, Heiko Keller, Jens Wöhnert, Lars T. Kuhn, Alexander L. Breeze, Wolfgang Bermel, Harald Schwalbe, Boris Fürtig
Pubblicato in: Journal of Biomolecular NMR, Numero 69/1, 2017, Pagina/e 31-44, ISSN 0925-2738
Editore: Kluwer Academic Publishers
DOI: 10.1007/s10858-017-0132-7

An effective introduction to structural crystallography using 1D Gaussian atoms

Autori: Emily Smith, Gwyndaf Evans, James Foadi
Pubblicato in: European Journal of Physics, Numero 38/6, 2017, Pagina/e 065501, ISSN 0143-0807
Editore: Institute of Physics Publishing
DOI: 10.1088/1361-6404/aa8188

Saposin-Lipoprotein Scaffolds for Structure Determination of Membrane Transporters

Autori: Joseph A. Lyons, Andreas Bøggild, Poul Nissen, Jens Frauenfeld
Pubblicato in: Methods in Enzymology, Numero 19/07/2017, 2017, Pagina/e 85-99
Editore: Elsevier
DOI: 10.1016/bs.mie.2017.06.035

È in corso la ricerca di dati su OpenAIRE...

Risultati finali

Pubblicazioni

Condividi questa pagina

Scarica