Periodic Reporting for period 4 - RECEPIANCE (Molecular reconstitution of epigenetic centromere inheritance)
Periodo di rendicontazione: 2020-02-01 al 2020-07-31
RECEPIANCE addresses a fundamental question on the molecular basis of genetic inheritance, which is of absolute importance for life. Understanding the molecular mechanisms of the process of chromosome segregation will also shed light on defects that lead to genetic imbalances that are typically found in human solid tumours.
The overall objective of RECEPIANCE is to perform an in vitro biochemical reconstitution of a crucial aspect of cell division. The appeal of biochemical reconstitutions is that they allow isolating and interrogating the crucial factors required to perform a given biochemical process. This, in turn, is instrumental to dissect the molecular function of such factors. In particular, RECEPIANCE aims to identify all factors required for the assembly of the structures involved in chromosome segregation, and their combination in a test tube in the presence of a source of energy that allows required biochemical work to be performed. This will not only generate a part list of the factors involved in this process, but will also allow addressing their interactions, physical relationships, and relative importance. Thus, the overall objective of RECEPIANCE is to gain a full understanding of the molecular basis of cell division.