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European Sepsis Academy: towards new biomarkers to improve sepsis management

Deliverables

Validation of the array used for whole blood leukocyte DNA analyses

Techniques used include in vivo and in vitro models of sepsis and immunosuppression; leukocyte purification and functional assays; FACS analysis; ELISA; molecular biology; microscopy.

Assessment of sequential trial versus a more standard clinical trial
Assessment of epigenetic signatures and DNA methylation marks in sepsis
Signed PCDPs

ESR's PCDP's

Analysis of markers such as PTX3marker, TIR8 and MSF as markers for the anti inflammatory phase of sepsis
Identification of potential utility of therapies (by confirmation of reversibility) directed towards mitochondrial protection, or stimulation of mitochondrial turnover
Design of a fully integrated molecular tool to manage sepsis patients based on their immune status: a proof of concept
ESR training certificates granted to ESRs
Further caracterisation of MDSC subpopulations
Data on patient flows and sepsis provided by partners for development of computer model.
Report of studies with PTX3, TIR8 and IRAK-M targeted mice in murine models of sepsis
Technology set up for the of DNA methylation probes of isolated cell fractions which can be used as prognostic markers in sepsis patients
Impact of putative therapies on reversing immune dysfunction in white cells
Validation of the primers and setup the Fluidigm technology for analysing MDSC
Proof of principle on testing therapeutic potential of PTX3 and IRAK-M modulation and potential identification of early biomarkers of immune dysfunction
Comparison of data from cell- and in vivo models to data obtained from human sepsis samples and confirmation of a relationship (both temporal and severity) between immune and mitochondrial function in white cell subsets
Validation of DNA methylation probes of isolated cell fractions
Temporal comparison of immune and bioenergetic function in models of sepsis
Characterisation of the involvement of factors such as IRAK-M, PTX3 and TIR8 in sepsis-induced immunosuppression

Partner ICH is in the lead for the characterisation of involvement of factors such as IRAK-M, PTX3 and TIR8 in sepsis-induced immunosuppression. AMC is the other partner involved.

Validation of PTX3, TIR8 and MSF as novel biomarkers of sepsis, to be translated to the clinic
First online version of network public multi modality website

First online version of network public website (with multimodality options)

Publications

Host cystathionine-γ lyase derived hydrogen sulfide protects against Pseudomonas aeruginosa sepsis.

Author(s): Georgios Renieris; Dionysia-Eirini Droggiti; Konstantina Katrini; Panagiotis Koufargyris; Theologia Gkavogianni; Eleni Karakike; Nikolaos Antonakos; Georgia Damoraki; Athanasios Karageorgos; Labros Sabracos; Antonia Katsouda; Elisa Jentho; Sebastian Weis; Rui Wang; Michael Bauer; Csaba Szabo; Kalliopi Platoni; Vasilios Kouloulias; Andreas Papapetropoulos; Evangelos J. Giamarellos-Bourboulis
Published in: PLoS Pathogens, Issue 5, 2021, ISSN 1553-7366
Publisher: Public Library of Science
DOI: 10.1371/journal.ppat.1009473

Pulmonary and intestinal microbiota dynamics during Gram-negative pneumonia-derived sepsis

Author(s): Wolff, Nora S.; Jacobs, Max C.; Wiersinga, W. Joost; Hugenholtz, Floor
Published in: Intensive Care Medicine Experimental, Vol 9, Iss 1, Pp 1-14 (2021), Issue 21, 2021, ISSN 2197-425X
Publisher: Springer Open
DOI: 10.1186/s40635-021-00398-4

ICU capacity management during the COVID-19 pandemic using a process simulation.

Author(s): Alban, Andres; Chick, Stephen E.; Dongelmans, Dave A.; Vlaar, Alexander P. J.; Sent, Danielle; van der Sluijs, Alexander F.; Wiersinga, W. Joost
Published in: Intensive care medicine, 46(8), 1624 - 1626. Springer Verlag, Issue 27, 2020, ISSN 0342-4642
Publisher: Springer Verlag
DOI: 10.1007/s00134-020-06066-7

Staphylococcus Aureus Osteomyelitis as an Inducer of Tolerance to Escherichia Coli Pyelonephritis: an Experimental Study

Author(s): Stavros Goumenos; Olga D. Savvidou; George Renieris; Theologia Gkavogianni; Panayiotis J. Papagelopoulos; Evangelos J. Giamarellos-Bourboulis
Published in: Scientific Reports, Vol 10, Iss 1, Pp 1-8 (2020), Issue 30, 2020, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-020-58420-w

Source of Circulating Pentraxin 3 in Septic Shock Patients.

Author(s): Chloé Albert Vega; Chloé Albert Vega; Marine Mommert; Marine Mommert; Mathilde Boccard; Mathilde Boccard; Thomas Rimmelé; Thomas Rimmelé; Fabienne Venet; Fabienne Venet; Alexandre Pachot; Veronique Leray; Guillaume Monneret; Guillaume Monneret; Benjamin Delwarde; Karen Brengel-Pesce; Karen Brengel-Pesce; François Mallet; François Mallet; François Mallet; Sophie Trouillet-Assant; Sophie Trou
Published in: Frontiers in Immunology, Issue 4, 2019, ISSN 1664-3224
Publisher: Frontiers media SA
DOI: 10.3389/fimmu.2018.03048

Immune Profiling Panel: a proof of concept study of a new multiplex molecular tool to assess the immune status of critically-ill patients

Author(s): Tawfik, Dina M.; Vachot, Laurence; Bocquet, Adeline; Venet, Fabienne; Rimmelé, Thomas; Monneret, Guillaume; Blein, Sophie; Montogomery, Jesse L.; Hemmert, Andrew C.; Pachot, Alexandre; Moucadel, Virginie; Marcos, Javier Yugueros; Brengel-Pesce, Karen; Mallet, François; Textoris, Julien
Published in: The Journal of Infectious Diseases, Issue 19, 2020, ISSN 1537-6613
Publisher: Oxford University press
DOI: 10.1093/infdis/jiaa248

Melioidosis

Author(s): W. Joost Wiersinga, Harjeet S. Virk, Alfredo G. Torres, Bart J. Currie, Sharon J. Peacock, David A. B. Dance, Direk Limmathurotsakul
Published in: Nature Reviews Disease Primers, Issue 4, 2018, Page(s) 17107, ISSN 2056-676X
Publisher: Nature reviews
DOI: 10.1038/nrdp.2017.107

The emerging role of the microbiota in the ICU

Author(s): Nora Suzanne Wolff, Floor Hugenholtz, Willem Joost Wiersinga
Published in: Critical Care, Issue 22/1, 2018, ISSN 1364-8535
Publisher: BioMed Central- Springer nature
DOI: 10.1186/s13054-018-1999-8

Sirtuin 2 Deficiency Increases Bacterial Phagocytosis by Macrophages and Protects from Chronic Staphylococcal Infection

Author(s): Eleonora Ciarlo, Tytti Heinonen, Charlotte Théroude, Jacobus Herderschee, Matteo Mombelli, Jérôme Lugrin, Marc Pfefferlé, Beatrice Tyrrell, Sarah Lensch, Hans Acha-Orbea, Didier Le Roy, Johan Auwerx, Thierry Roger
Published in: Frontiers in Immunology, Issue 8, 2017, ISSN 1664-3224
Publisher: Frontiers in Immunology
DOI: 10.3389/fimmu.2017.01037

Immune Functional Assays, From Custom to Standardized Tests for Precision Medicine

Author(s): Chloé Albert-Vega, Dina M. Tawfik, Sophie Trouillet-Assant, Laurence Vachot, François Mallet, Julien Textoris
Published in: Frontiers in Immunology, Issue 9, 2018, Page(s) 1-9, ISSN 1664-3224
Publisher: Frontiers
DOI: 10.3389/fimmu.2018.02367

Sirtuin 5 Deficiency Does Not Compromise Innate Immune Responses to Bacterial Infections

Author(s): Tytti Heinonen, Eleonora Ciarlo, Charlotte Théroude, Aimilia Pelekanou, Jacobus Herderschee, Didier Le Roy, Thierry Roger
Published in: Frontiers in Immunology, Issue 9, 2018, ISSN 1664-3224
Publisher: Frontiers
DOI: 10.3389/fimmu.2018.02675

Late Peaks of HMGB1 and Sepsis Outcome

Author(s): Eleni Karakike, Maria-Evangelia Adami, Malvina Lada, Theologia Gkavogianni, Ioannis M. Koutelidakis, Michael Bauer, Evangelos J. Giamarellos-Bourboulis, Iraklis Tsangaris
Published in: SHOCK, 2019, Page(s) 1, ISSN 1073-2322
Publisher: Lippincott Williams & Wilkins Ltd.
DOI: 10.1097/shk.0000000000001265

Myeloid-Derived Suppressor Cells in Sepsis

Author(s): Irene T. Schrijver, Charlotte Théroude, Thierry Roger
Published in: Frontiers in Immunology, Issue 10, 2019, ISSN 1664-3224
Publisher: Frontiers Media SA
DOI: 10.3389/fimmu.2019.00327

Macrophage Activation-Like Syndrome: A Distinct Entity Leading to Early Death in Sepsis

Author(s): Eleni Karakike, Evangelos J. Giamarellos-Bourboulis
Published in: Frontiers in Immunology, Issue 10, 2019, ISSN 1664-3224
Publisher: Frontiers Media SA
DOI: 10.3389/fimmu.2019.00055

Trained immunity confers broad-spectrum protection against bacterial infections

Author(s): Eleonora Ciarlo, Tytti Heinonen, Charlotte Théroude, Fatemeh Asgari, Didier Le Roy, Mihai G Netea, Thierry Roger
Published in: The Journal of Infectious Diseases, 2019, ISSN 0022-1899
Publisher: University of Chicago Press
DOI: 10.1093/infdis/jiz692

The early change of SOFA score as a prognostic marker of 28-day sepsis mortality: analysis through a derivation and a validation cohort

Author(s): Eleni Karakike, Evdoxia Kyriazopoulou, Iraklis Tsangaris, Christina Routsi, Jean-Louis Vincent, Evangelos J. Giamarellos-Bourboulis
Published in: Critical Care, Issue 23/1, 2019, ISSN 1364-8535
Publisher: Springer Nature
DOI: 10.1186/s13054-019-2665-5

Daptomycin as adjunctive treatment for experimental infection by Acinetobacter baumannii with resistance to colistin

Author(s): Garyfallia Poulakou, Georgios Renieris, Labros Sabrakos, Olympia Zarkotou, Katherine Themeli-Digalaki, Efstathia Perivolioti, Eleni Kraniotaki, Evangelos J. Giamarellos-Bourboulis, Nikolaos Zavras
Published in: International Journal of Antimicrobial Agents, Issue 53/2, 2019, Page(s) 190-194, ISSN 0924-8579
Publisher: Elsevier BV
DOI: 10.1016/j.ijantimicag.2018.10.024

Early increase of VEGF-A is associated with resolution of ventilator-associated pneumonia: Clinical and experimental evidence

Author(s): Ioannis Strouvalis, Christina Routsi, Maria Adamopoulou, Maria Raftogiannis, George Renieris, Stylianos E. Orfanos, Anastasia Kotanidou, Labros Sabracos, Evangelos J. Giamarellos-Bourboulis
Published in: Respirology, Issue 23/10, 2018, Page(s) 942-949, ISSN 1323-7799
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/resp.13320

Staphylococcus Aureus Osteomyelitis as an Inducer of Tolerance to Escherichia Coli Pyelonephritis: an Experimental Study

Author(s): Stavros Goumenos, Olga Savvidou, George Renieris, Theologia Gkavogianni, Panayiotis J. Papagelopoulos, Evangelos J. Giamarellos-Bourboulis
Published in: Scientific Reports, Issue 10/1, 2020, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-020-58420-w

The Long Pentraxin PTX3 as a Humoral Innate Immunity Functional Player and Biomarker of Infections and Sepsis

Author(s): Rémi Porte, Sadaf Davoudian, Fatemeh Asgari, Raffaella Parente, Alberto Mantovani, Cecilia Garlanda, Barbara Bottazzi
Published in: Frontiers in Immunology, Issue 10, 2019, ISSN 1664-3224
Publisher: Europa PMC
DOI: 10.3389/fimmu.2019.00794

Association of modulation of pro‐inflammatory responses by dectin‐2 with preterm delivery: An experimental model

Author(s): Aspasia Liassidou, Georgios Renieris, Dionysia‐Irene Droggiti, Theologia Gkavogianni, Christakis Liassides, Labros Sabracos, Evangelos J. Giamarellos‐Bourboulis, Charalampos S. Siristatidis
Published in: American Journal of Reproductive Immunology, Issue 83/3, 2019, ISSN 1046-7408
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/aji.13216

Impact of antimicrobial therapy on the gut microbiome

Author(s): Amira A Bhalodi, Tjitske S R van Engelen, Harjeet S Virk, W Joost Wiersinga
Published in: Journal of Antimicrobial Chemotherapy, Issue 74/Supplement_1, 2019, Page(s) i6-i15, ISSN 0305-7453
Publisher: Oxford University Press
DOI: 10.1093/jac/dky530

Global burden of melioidosis in 2015: a systematic review and data synthesis

Author(s): Emma Birnie, Harjeet S Virk, Jelmer Savelkoel, Rene Spijker, Eric Bertherat, David A B Dance, Direk Limmathurotsakul, Brecht Devleesschauwer, Juanita A Haagsma, W Joost Wiersinga
Published in: The Lancet Infectious Diseases, Issue 19/8, 2019, Page(s) 892-902, ISSN 1473-3099
Publisher: The Lancet Publishing Group
DOI: 10.1016/s1473-3099(19)30157-4

Macrophage migration inhibitory factor promotes the migration of dendritic cells through CD74 and the activation of the Src/PI3K/myosin II pathway.

Author(s): Annette Ives; Didier Le Roy; Charlotte Théroude; Jürgen Bernhagen; Thierry Roger; Thierry Calandra
Published in: Crossref, Issue 37, 2021, ISSN 0892-6638
Publisher: Federation of American Societies for Experimental Biology
DOI: 10.1096/fj.202001605r

Vendor effects on murine gut microbiota and its influence on lipopolysaccharide-induced lung inflammation and Gram-negative pneumonia

Author(s): Nora S. Wolff; Max C. Jacobs; Bastiaan W. Haak; Joris J. T. H. Roelofs; Alex F. de Vos; Floor Hugenholtz; W. Joost Wiersinga
Published in: Intensive Care Medicine Experimental volume, Issue 8, 47, 2020, ISSN 2197-425X
Publisher: Springer open
DOI: 10.1186/s40635-020-00336-w

The Long Pentraxin PTX3 Controls Klebsiella Pneumoniae Severe Infection

Author(s): Fatemeh Asgari; Fatemeh Asgari; Domenico Supino; Raffaella Parente; Nadia Polentarutti; Matteo Stravalaci; Matteo Stravalaci; Remi Porte; Fabio Pasqualini; Fabio Pasqualini; Marialuisa Barbagallo; Chiara Perucchini; Camilla Recordati; Elena Magrini; Andrea Mariancini; Federica Riva; Alessia Giordano; Sadaf Davoudian; Sadaf Davoudian; Thierry Roger; Cornelis van’t Veer; Sebastien Jaillon; Sebasti
Published in: Frontiers in immunology, vol. 12, pp. 666198, Issue 34, 2021, ISSN 1664-3224
Publisher: Frontiers Media SA
DOI: 10.3389/fimmu.2021.666198

Cancer Immunology - A Translational Medicine Context

Author(s): Rezaei N. Authors. Tamara Gulic, Rita Silva-Gomes, Sadaf Davoudian, Marina Sironi, Paola Allavena, Alberto Mantovani, Barbara Bottazzi.
Published in: 2020, ISBN 978-3-030-30845-2
Publisher: Springer, Cham
DOI: 10.1007/978-3-030-30845-2

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