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Study of the interplay between CRISPR interference and DNA repair pathways towards the development of novel CRISPR tools


A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9

Author(s): Lun Cui, Antoine Vigouroux, François Rousset, Hugo Varet, Varun Khanna, David Bikard
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
DOI: 10.1038/s41467-018-04209-5

Tuning dCas9's ability to block transcription enables robust, noiseless knockdown of bacterial genes

Author(s): Antoine Vigouroux, Enno Oldewurtel, Lun Cui, David Bikard, Sven van Teeffelen
Published in: Molecular Systems Biology, Issue 14/3, 2018, Page(s) e7899, ISSN 1744-4292
DOI: 10.15252/msb.20177899

Inhibition of NHEJ repair by type II-A CRISPR-Cas systems in bacteria

Author(s): Aude Bernheim, Alicia Calvo-Villamañán, Clovis Basier, Lun Cui, Eduardo P. C. Rocha, Marie Touchon, David Bikard
Published in: Nature Communications, Issue 8/1, 2017, ISSN 2041-1723
DOI: 10.1038/s41467-017-02350-1

Using CRISPR-Cas systems as antimicrobials

Author(s): David Bikard, Rodolphe Barrangou
Published in: Current Opinion in Microbiology, Issue 37, 2017, Page(s) 155-160, ISSN 1369-5274
DOI: 10.1016/j.mib.2017.08.005

Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors

Author(s): François Rousset, Lun Cui, Elise Siouve, Christophe Becavin, Florence Depardieu, David Bikard
Published in: PLOS Genetics, Issue 14/11, 2018, Page(s) e1007749, ISSN 1553-7404
DOI: 10.1371/journal.pgen.1007749