Study of the interplay between CRISPR interference and DNA repair pathways towards the development of novel CRISPR tools

Publikacje

The impact of genetic diversity on gene essentiality within the Escherichia coli species

Autorzy: François Rousset, Jose Cabezas-Caballero, Florence Piastra-Facon, Jesús Fernández-Rodríguez, Olivier Clermont, Erick Denamur, Eduardo P. C. Rocha, David Bikard
Opublikowane w: Nature Microbiology, Numer 20585276, 2021, ISSN 2058-5276
Wydawca: Nature Microbiology
DOI: 10.1038/s41564-020-00839-y

CRISPR Tools To Control Gene Expression in Bacteria

Autorzy: Antoine Vigouroux, David Bikard
Opublikowane w: Microbiology and Molecular Biology Reviews, Numer 84/2, 2020, ISSN 1092-2172
Wydawca: American Society for Microbiology
DOI: 10.1128/mmbr.00077-19

Class-A penicillin binding proteins do not contribute to cell shape but repair cell-wall defects

Autorzy: Antoine Vigouroux, Baptiste Cordier, Andrey Aristov, Laura Alvarez, Gizem Özbaykal, Thibault Chaze, Enno Rainer Oldewurtel, Mariette Matondo, Felipe Cava, David Bikard, Sven van Teeffelen
Opublikowane w: eLife, Numer 9, 2020, ISSN 2050-084X
Wydawca: eLife Sciences Publications
DOI: 10.7554/eLife.51998

Lipoprotein DolP supports proper folding of BamA in the bacterial outer membrane, promoting fitness upon envelope stress.

Autorzy: David Ranava; Yiying Yang; Luis Orenday-Tapia; François Rousset; Catherine Turlan; Violette Morales; Lun Cui; Cyril Moulin; Carine Froment; Gladys Munoz; Jérôme Rech; Julien Marcoux; Anne Caumont-Sarcos; Cécile Albenne; David Bikard; Raffaele Ieva
Opublikowane w: Elife, Numer 2050084X, 2021, ISSN 2050-084X
Wydawca: eLife Sciences Publications
DOI: 10.7554/elife.67817

Gene silencing with CRISPRi in bacteria and optimization of dCas9 expression levels

Autorzy: Florence Depardieu, David Bikard
Opublikowane w: Methods, Numer 172, 2020, Strona(/y) 61-75, ISSN 1046-2023
Wydawca: Academic Press
DOI: 10.1016/j.ymeth.2019.07.024

A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9

Autorzy: Lun Cui, Antoine Vigouroux, François Rousset, Hugo Varet, Varun Khanna, David Bikard
Opublikowane w: Nature Communications, Numer 9/1, 2018, ISSN 2041-1723
Wydawca: Nature Publishing Group
DOI: 10.1038/s41467-018-04209-5

Tuning dCas9's ability to block transcription enables robust, noiseless knockdown of bacterial genes

Autorzy: Antoine Vigouroux, Enno Oldewurtel, Lun Cui, David Bikard, Sven van Teeffelen
Opublikowane w: Molecular Systems Biology, Numer 14/3, 2018, Strona(/y) e7899, ISSN 1744-4292
Wydawca: Nature Publishing Group
DOI: 10.15252/msb.20177899

Inhibition of NHEJ repair by type II-A CRISPR-Cas systems in bacteria

Autorzy: Aude Bernheim, Alicia Calvo-Villamañán, Clovis Basier, Lun Cui, Eduardo P. C. Rocha, Marie Touchon, David Bikard
Opublikowane w: Nature Communications, Numer 8/1, 2017, ISSN 2041-1723
Wydawca: Nature Publishing Group
DOI: 10.1038/s41467-017-02350-1

Using CRISPR-Cas systems as antimicrobials

Autorzy: David Bikard, Rodolphe Barrangou
Opublikowane w: Current Opinion in Microbiology, Numer 37, 2017, Strona(/y) 155-160, ISSN 1369-5274
Wydawca: Elsevier BV
DOI: 10.1016/j.mib.2017.08.005

Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors

Autorzy: François Rousset, Lun Cui, Elise Siouve, Christophe Becavin, Florence Depardieu, David Bikard
Opublikowane w: PLOS Genetics, Numer 14/11, 2018, Strona(/y) e1007749, ISSN 1553-7404
Wydawca: PLOS
DOI: 10.1371/journal.pgen.1007749

Editing the microbiome the CRISPR way

Autorzy: Gayetri Ramachandran, David Bikard
Opublikowane w: Philosophical Transactions of the Royal Society B: Biological Sciences, Numer 374/1772, 2019, Strona(/y) 20180103, ISSN 0962-8436
Wydawca: Royal Society of London
DOI: 10.1098/rstb.2018.0103

A matter of background: DNA repair pathways as a possible cause for the sparse distribution of CRISPR-Cas systems in bacteria

Autorzy: Aude Bernheim, David Bikard, Marie Touchon, Eduardo P. C. Rocha
Opublikowane w: Philosophical Transactions of the Royal Society B: Biological Sciences, Numer 374/1772, 2019, Strona(/y) 20180088, ISSN 0962-8436
Wydawca: Royal Society of London
DOI: 10.1098/rstb.2018.0088

CRISPR screens in the era of microbiomes

Autorzy: François Rousset, David Bikard
Opublikowane w: Current Opinion in Microbiology, Numer 57, 2020, Strona(/y) 70-77, ISSN 1369-5274
Wydawca: Elsevier BV
DOI: 10.1016/j.mib.2020.07.009

On-target activity predictions enable improved CRISPR–dCas9 screens in bacteria

Autorzy: Alicia Calvo-Villamañán, Jérome Wong Ng, Rémi Planel, Hervé Ménager, Arthur Chen, Lun Cui, David Bikard
Opublikowane w: Nucleic Acids Research, Numer 48/11, 2020, Strona(/y) e64-e64, ISSN 0305-1048
Wydawca: Oxford University Press
DOI: 10.1093/nar/gkaa294

Atypical organizations and epistatic interactions of CRISPRs and cas clusters in genomes and their mobile genetic elements

Autorzy: Aude Bernheim, David Bikard, Marie Touchon, Eduardo P C Rocha
Opublikowane w: Nucleic Acids Research, 2019, ISSN 0305-1048
Wydawca: Oxford University Press
DOI: 10.1093/nar/gkz1091

Phages and their satellites encode hotspots of antiviral systems

Autorzy: Rousset F., Depardieu F., Miele S., Dowding J., Laval A.-L., Lieberman E., Garry D., Rocha E.P.C., Bernheim A., Bikard D.
Opublikowane w: Cell Host & Microbe, Numer 19313128, 2022, ISSN 1931-3128
Wydawca: Cell Press
DOI: 10.1016/j.chom.2022.02.018

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IMPROVING SEQUENCE-SPECIFIC ANTIMICROBIALS BY BLOCKING DNA REPAIR

Numer wniosku/publikacji: EP 16741899
Data: 2016-07-13

IMPROVING SEQUENCE-SPECIFIC ANTIMICROBIALS BY BLOCKING DNA REPAIR

Numer wniosku/publikacji: EP 16741899
Data: 2016-07-13

IMPROVING SEQUENCE-SPECIFIC ANTIMICROBIALS BY BLOCKING DNA REPAIR

Numer wniosku/publikacji: EP 16741899
Data: 2016-07-13
Wnioskodawca/wnioskodawcy: INSTITUT PASTEUR

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