The influence of the cell membrane asymmetry and curvature on the functioning of membrane proteins and the transport of therapeutic compounds

Project Information

assymcurv

Grant agreement ID: 690853

DOI 10.3030/690853

Project closed

EC signature date 8 December 2015

Start date 1 January 2016

End date 31 December 2019

Funded under

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Total cost € 202 500,00

EU contribution € 202 500,00

Coordinated by

INSTITUTE OF PHYSICS OF NATIONAL ACADEMY OF SCIENCE OF UKRAINE
Ukraine

Deliverables

Documents, reports (4)

Progress Report 1

Progress report

Coherent view of the project goals, methods and schedules

Coherent view of the project goals, methods and schedules among all project participants.

Progress report 2

Progress report

Publishing and dissemination of results

Other (6)

Curvature-dependent potentials of mean force for drug translocation through the membrane

Dat on curvature-dependent potentials of mean force for drug translocation through the membrane.

Empirical force fields of the drugs

Working files of empirical force fields of the drugs.

Experimental markers of conformation changes in membrane-protein and membrane-drug complexes.

Data on experimental markers of conformation changes in membrane-protein and membrane-drug complexes.

Mid-term meeting

There will be only one mid-term meeting per project with timing between month 14 and 24. Definitive date is to be confirmed by the coordinator and preferably linked to a consortium event.

Comparison of experimental and simulation results

Consistent view of the curvature- and asymmetry-induced phenomena in the membranes from experimental and simulation perspectives

Curvature-dependent conformational changes of the membrane proteins

Data on curvature-dependent conformational changes of the membrane proteins

Publications

Peer reviewed articles (18)

The influence of curvature on the properties of the plasma membrane. Insights from atomistic molecular dynamics simulations

Author(s): Semen O. Yesylevskyy, Timothée Rivel, Christophe Ramseyer
Published in: Scientific Reports, Issue 7/1, 2017, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-017-16450-x

Low density lipoproteins and human serum albumin as the carriers of squalenoylated drugs: insights from molecular simulations

Author(s): Semen O. Yesylevskyy, Christophe Ramseyer, Mariia Savenko, Simona Mura, Patrick Couvreur
Published in: Molecular Pharmaceutics, 2018, ISSN 1543-8384
Publisher: American Chemical Society
DOI: 10.1021/acs.molpharmaceut.7b00952

2D-BN nanoparticles as a spectroscopic marker and drug delivery system with protection properties

Author(s): O. P. Gnatyuk, G. I. Dovbeshko, A. Yershov, S. O. Karakhim, O. Ilchenko, O. Yu. Posudievsky
Published in: RSC Advances, Issue 8/53, 2018, Page(s) 30404-30411, ISSN 2046-2069
Publisher: Royal Society of Chemistry
DOI: 10.1039/c8ra05085f

Double-exponential kinetics of binding and redistribution of the fluorescent dyes in cell membranes witness for the existence of lipid microdomains

Author(s): Kyrylo A. Pyrshev, Semen O. Yesylevskyy, Alexander P. Demchenko
Published in: Biochemical and Biophysical Research Communications, Issue 508/4, 2019, Page(s) 1139-1144, ISSN 0006-291X
Publisher: Academic Press
DOI: 10.1016/j.bbrc.2018.12.054

Biogenesis, transport and remodeling of lysophospholipids in Gram-negative bacteria

Author(s): Lei Zheng, Yibin Lin, Shuo Lu, Jiazhe Zhang, Mikhail Bogdanov
Published in: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Issue 1862/11, 2017, Page(s) 1404-1413, ISSN 1388-1981
Publisher: Elsevier BV
DOI: 10.1016/j.bbalip.2016.11.015

The phospholipid-repair system LplT/Aas in Gram-negative bacteria protects the bacterial membrane envelope from host phospholipase A 2 attack

Author(s): Yibin Lin, Mikhail Bogdanov, Shuo Lu, Ziqiang Guan, William Margolin, Jerrold Weiss, Lei Zheng
Published in: Journal of Biological Chemistry, Issue 293/9, 2018, Page(s) 3386-3398, ISSN 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.ra117.001231

Effects of elevated growth temperature and heat shock on the lipid composition of the inner and outer membranes of Yersinia pseudotuberculosis

Author(s): Ludmila Davydova, Svetlana Bakholdina, Maria Barkina, Peter Velansky, Mikhail Bogdanov, Nina Sanina
Published in: Biochimie, Issue 123, 2016, Page(s) 103-109, ISSN 0300-9084
Publisher: Elsevier BV
DOI: 10.1016/j.biochi.2016.02.004

Structures of single, double and triple layers of lipids adsorbed on graphene: Insights from all-atom molecular dynamics simulations

Author(s): Timothée Rivel, Semen O. Yesylevskyy, Christophe Ramseyer
Published in: Carbon, Issue 118, 2017, Page(s) 358-369, ISSN 0008-6223
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.carbon.2017.03.072

Stacking as a Key Property for Creating Nanoparticles with Tunable Shape: The Case of Squalenoyl-Doxorubicin

Author(s): Julie Mougin, Semen O. Yesylevskyy, Claudie Bourgaux, David Chapron, Jean-Philippe Michel, Franco Dosio, Barbara Stella, Christophe Ramseyer, Patrick Couvreur
Published in: ACS Nano, Issue 13/11, 2019, Page(s) 12870-12879, ISSN 1936-0851
Publisher: American Chemical Society
DOI: 10.1021/acsnano.9b05303

Fate of cisplatin and its main hydrolysed forms in the presence of thiolates: a comprehensive computational and experimental study

Author(s): Thibault Minervini, Bruno Cardey, Sarah Foley, Christophe Ramseyer, Mironel Enescu
Published in: Metallomics, Issue 11/4, 2019, Page(s) 833-844, ISSN 1756-5901
Publisher: Royal Society of Chemistry
DOI: 10.1039/c8mt00371h

The asymmetry of plasma membranes and their cholesterol content influence the uptake of cisplatin

Author(s): Timothée Rivel, Christophe Ramseyer, Semen Yesylevskyy
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-019-41903-w

Curvature increases permeability of the plasma membrane for ions, water and the anti-cancer drugs cisplatin and gemcitabine

Author(s): Semen Yesylevskyy, Timothée Rivel, Christophe Ramseyer
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-019-53952-2

Squalene versus cholesterol: Which is the best nanocarrier for the delivery to cells of the anticancer drug gemcitabine?

Author(s): Arnaud Peramo, Simona Mura, Semen O. Yesylevskyy, Bruno Cardey, Dunja Sobot, Stephanie Denis, Christophe Ramseyer, Didier Desmaële, Patrick Couvreur
Published in: Comptes Rendus Chimie, Issue 21/10, 2018, Page(s) 974-986, ISSN 1631-0748
Publisher: Elsevier Masson
DOI: 10.1016/j.crci.2018.02.008

Conjugation of squalene to gemcitabine as unique approach exploiting endogenous lipoproteins for drug delivery

Author(s): Dunja Sobot, Simona Mura, Semen O. Yesylevskyy, Laura Dalbin, Fanny Cayre, Guillaume Bort, Julie Mougin, Didier Desmaële, Sinda Lepetre-Mouelhi, Grégory Pieters, Bohdan Andreiuk, Andrey S. Klymchenko, Jean-Louis Paul, Christophe Ramseyer, Patrick Couvreur
Published in: Nature Communications, Issue 8, 2017, Page(s) 15678, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/ncomms15678

Spectral and kinetic effects accompanying the assembly of core complexes of Rhodobacter sphaeroides

Author(s): Arvi Freiberg, Manoop Chenchiliyan, Margus Rätsep, Kõu Timpmann
Published in: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Issue 1857/11, 2016, Page(s) 1727-1733, ISSN 0005-2728
Publisher: Elsevier BV
DOI: 10.1016/j.bbabio.2016.08.001

Hydrogen bonds in the vicinity of the special pair of the bacterial reaction center probed by hydrostatic high-pressure absorption spectroscopy

Author(s): Liina Kangur, Michael R. Jones, Arvi Freiberg
Published in: Biophysical Chemistry, 2017, ISSN 0301-4622
Publisher: Elsevier BV
DOI: 10.1016/j.bpc.2017.04.003

High-pressure modulation of the structure of the bacterial photochemical reaction center at physiological and cryogenic temperatures

Author(s): Kõu Timpmann, Liina Kangur, Ants Lõhmus, Arvi Freiberg
Published in: Journal of Physics B: Atomic, Molecular and Optical Physics, Issue 50/14, 2017, Page(s) 144006, ISSN 0953-4075
Publisher: Institute of Physics Publishing
DOI: 10.1088/1361-6455/aa77e4

Dimerization of core complexes as an efficient strategy for energy trapping in Rhodobacter sphaeroides

Author(s): Manoop Chenchiliyan, Kõu Timpmann, Erko Jalviste, Peter G. Adams, C. Neil Hunter, Arvi Freiberg
Published in: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Issue 1857/6, 2016, Page(s) 634-642, ISSN 0005-2728
Publisher: Elsevier BV
DOI: 10.1016/j.bbabio.2016.03.020