Prenylated-flavins: Application and Biochemistry

Project Information

pre-FAB

Grant agreement ID: 695013

DOI 10.3030/695013

Project closed

EC signature date 18 April 2016

Start date 1 September 2016

End date 31 August 2023

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 2 494 328,75

EU contribution € 2 494 328,75

Coordinated by

THE UNIVERSITY OF MANCHESTER
United Kingdom

Publications

Peer reviewed articles (21)

Enzymatic C–H activation of aromatic compounds through CO2 fixation

Author(s): Godwin A. Aleku, Annica Saaret, Ruth T. Bradshaw-Allen, Sasha R. Derrington, Gabriel R. Titchiner, Irina Gostimskaya, Deepankar Gahloth, David A. Parker, Sam Hay, David Leys
Published in: Nature Chemical Biology, Issue 16/11, 2020, Page(s) 1255-1260, ISSN 1552-4450
Publisher: Nature Publishing Group
DOI: 10.1038/s41589-020-0603-0

Directed evolution of prenylated FMN-dependent Fdc supports efficient in vivo isobutene production

Author(s): Annica Saaret, Benoît Villiers, François Stricher, Macha Anissimova, Mélodie Cadillon, Reynard Spiess, Sam Hay, David Leys
Published in: Nature Communications, 2021, Page(s) 12, 5300, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-25598-0

Ferulic Acid Decarboxylase Controls Oxidative Maturation of the Prenylated Flavin Mononucleotide Cofactor

Author(s): Arune Balaikaite, Malama Chisanga, Karl Fisher, Derren J. Heyes, Reynard Spiess, David Leys
Published in: ACS Chemical Biology, Issue 15/9, 2020, Page(s) 2466-2475, ISSN 1554-8929
Publisher: American Chemical Society
DOI: 10.1021/acschembio.0c00456

Prenylated flavins: structures and mechanisms

Author(s): Samuel Bloor; Iaroslav Michurin; Gabriel R. Titchiner; David Leys
Published in: The FEBS Journal, 2023, Page(s) 2232-2245, ISSN 1742-464X
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/febs.16371

UbiD domain dynamics underpins aromatic decarboxylation

Author(s): Stephen A. Marshall, Karl A.P. Payne, Karl Fisher, GR Titchiner, C Levy, S Hay, D Leys
Published in: Nature Communications, 2021, Page(s) 12, 5056, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-25278-z

The UbiX flavin prenyltransferase reaction mechanism resembles class I terpene cyclase chemistry

Author(s): Stephen A. Marshall, Karl A. P. Payne, Karl Fisher, Mark D. White, Aisling Ní Cheallaigh, Arune Balaikaite, Stephen E. J. Rigby, David Leys
Published in: Nature Communications, Issue 10/1, 2019, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-019-10220-1

Enzymatic <i>N</i>-Allylation of Primary and Secondary Amines Using Renewable Cinnamic Acids Enabled by Bacterial Reductive Aminases

Author(s): Godwin A. Aleku; Gabriel R. Titchiner; George W. Roberts; Sasha R. Derrington; James R. Marshall; Florian Hollfelder; Nicholas J. Turner; David Leys
Published in: ACS Susainable Chemistry and Engineering, Issue 9, 2022, ISSN 2168-0485
Publisher: American Chemical Society
DOI: 10.1021/acssuschemeng.2c01180

Stability engineering of ferulic acid decarboxylase unlocks enhanced aromatic acid decarboxylation

Author(s): George W Roberts, Karl Fisher, Tom Jowitt, David Leys
Published in: Current Research in Chemical Biology, 2023, ISSN 2666-2469
Publisher: Elsevier
DOI: 10.1016/j.crchbi.2023.100043

The role of conserved residues in Fdc decarboxylase in prenylated flavin mononucleotide oxidative maturation, cofactor isomerization, and catalysis

Author(s): Samuel S. Bailey, Karl A. P. Payne, Karl Fisher, Stephen A. Marshall, Matthew J. Cliff, Reynard Spiess, David A. Parker, Stephen E. J. Rigby, David Leys
Published in: Journal of Biological Chemistry, Issue 293/7, 2018, Page(s) 2272-2287, ISSN 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.RA117.000881

The UbiX-UbiD system: The biosynthesis and use of prenylated flavin (prFMN)

Author(s): Stephen A. Marshall, Karl A.P. Payne, David Leys
Published in: Archives of Biochemistry and Biophysics, Issue 632, 2017, Page(s) 209-221, ISSN 0003-9861
Publisher: Academic Press
DOI: 10.1016/j.abb.2017.07.014

Regioselective para -Carboxylation of Catechols with a Prenylated Flavin Dependent Decarboxylase

Author(s): Stefan E. Payer, Stephen A. Marshall, Natalie Bärland, Xiang Sheng, Tamara Reiter, Andela Dordic, Georg Steinkellner, Christiane Wuensch, Susann Kaltwasser, Karl Fisher, Stephen E. J. Rigby, Peter Macheroux, Janet Vonck, Karl Gruber, Kurt Faber, Fahmi Himo, David Leys, Tea Pavkov-Keller, Silvia M. Glueck
Published in: Angewandte Chemie International Edition, Issue 56/44, 2017, Page(s) 13893-13897, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201708091

Enzymatic Carboxylation of 2-Furoic Acid Yields 2,5-Furandicarboxylic Acid (FDCA)

Author(s): Karl A.P. Payne, Stephen A. Marshall, Karl Fisher, Matthew J. Cliff, Diego M. Cannas, Cunyu Yan, Derren J. Heyes, David A. Parker, Igor Larrosa, David Leys
Published in: ACS Catalysis, Issue 9/4, 2019, Page(s) 2854-2865, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.8b04862

Flavin metamorphosis: cofactor transformation through prenylation

Author(s): David Leys
Published in: Current Opinion in Chemical Biology, Issue 47, 2018, Page(s) 117-125, ISSN 1367-5931
Publisher: Elsevier BV
DOI: 10.1016/j.cbpa.2018.09.024

Terminal Alkenes from Acrylic Acid Derivatives via Non-Oxidative Enzymatic Decarboxylation by Ferulic Acid Decarboxylases

Author(s): Godwin A. Aleku, Christoph Prause, Ruth T. Bradshaw-Allen, Katharina Plasch, Silvia M. Glueck, Samuel S. Bailey, Karl A. P. Payne, David A. Parker, Kurt Faber, David Leys
Published in: ChemCatChem, Issue 10/17, 2018, Page(s) 3736-3745, ISSN 1867-3880
Publisher: Wiley - VCH Verlag GmbH & CO. KGaA
DOI: 10.1002/cctc.201800643

Enzymatic control of cycloadduct conformation ensures reversible 1,3-dipolar cycloaddition in a prFMN-dependent decarboxylase

Author(s): Samuel S. Bailey, Karl A. P. Payne, Annica Saaret, Stephen A. Marshall, Irina Gostimskaya, Iaroslav Kosov, Karl Fisher, Sam Hay, David Leys
Published in: Nature Chemistry, Issue 11/11, 2019, Page(s) 1049-1057, ISSN 1755-4330
Publisher: Nature Publishing Group
DOI: 10.1038/s41557-019-0324-8

Structural and biochemical characterization of the prenylated flavin mononucleotide-dependent indole-3-carboxylic acid decarboxylase

Author(s): Deepankar Gahloth; Karl Fisher; Karl A.P. Payne; Matthew Cliff; Colin Levy; David Leys
Published in: Journal of Biological Chemistry, Issue 4, 2022, ISSN 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1016/j.jbc.2022.101771

Structural insights into UbiD reversible decarboxylation

Author(s): George W. Roberts; David Leys
Published in: Current Opinion in Structural Biology, Issue 8, 2022, ISSN 0959-440X
Publisher: Elsevier BV
DOI: 10.1016/j.sbi.2022.102432

Synthetic Enzyme‐Catalyzed CO 2 Fixation Reactions

Author(s): Godwin A. Aleku, George W. Roberts, Gabriel R. Titchiner, David Leys
Published in: ChemSusChem, Issue 14/8, 2021, Page(s) 1781-1804, ISSN 1864-5631
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/cssc.202100159

Structure and Mechanism of Pseudomonas aeruginosa PA0254/HudA, a prFMN-Dependent Pyrrole-2-carboxylic Acid Decarboxylase Linked to Virulence

Author(s): Karl A. P. Payne, Stephen A. Marshall, Karl Fisher, Stephen E. J. Rigby, Matthew J. Cliff, Reynard Spiess, Diego M. Cannas, Igor Larrosa, Sam Hay, David Leys
Published in: ACS Catalysis, Issue 11/5, 2021, Page(s) 2865-2878, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.0c05042

Biocatalytic reduction of α,β-unsaturated carboxylic acids to allylic alcohols

Author(s): Godwin A. Aleku, George W. Roberts, David Leys
Published in: Green Chemistry, Issue 22/12, 2020, Page(s) 3927-3939, ISSN 1463-9262
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0gc00867b

Biosynthesis of Pyrrole-2-carbaldehyde via Enzymatic CO2 Fixation

Author(s): Gabriel R. Titchiner; Stephen A. Marshall; Herkus Miscikas; David Leys
Published in: Catalysts, 2022, Page(s) 12, 538, ISSN 2073-4344
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/catal12050538