Molecular Evolution of the Primary Structure of Single Chain Polymer Nanoparticles via Dynamic Covalent Chemistry

Información del proyecto

MolecularEVOLUTION

Identificador del acuerdo de subvención: 705701

DOI

10.3030/705701

Proyecto cerrado

Fecha de la firma de la CE 24 Febrero 2016

Fecha de inicio 1 Marzo 2016

Fecha de finalización 28 Febrero 2018

Financiado con arreglo a

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Coste total

€ 165 598,80

Aportación de la UE

€ 165 598,80

Coordinado por

TECHNISCHE UNIVERSITEIT EINDHOVEN
Netherlands

Objetivo

Cancer continues to be a major cause of death worldwide. While many drugs can reduce the size of tumors, their side effects commonly outweigh their benefits when the drug is administered by conventional methods. One promising approach to overcome this issue is to employ a synthetic enzyme to activate a prodrug into an active drug via a bioorthogonal reaction at the site of a tumor within a patient. Recent work by the group of Prof. E. W. Meijer at the Eindhoven University of Technology on amphiphilic polymers with pendant “sticky” hydrogen bonding moieties and pendant catalytic centers represents state-of-the-art synthetic enzymes. When these polymers are dissolved in dilute aqueous solutions, individual chains fold to form single chain polymer nanoparticles (SCPNs). The folding is thermodynamically driven by hydrophobic interactions and the dynamic aggregation of the “sticky” moieties. Although current SCPNs catalyze bioorthogonal reactions in water, they do not have well-defined high order structure like natural enzymes, instead exhibiting non-cooperative folding and open, elliptical structures. This lack of well-defined high order structure is due to a lack of polymer sequence control, as SCPNs reported to date are random or block copolymers. As a postdoctoral fellow in the Meijer group, I propose to make a new generation of biocompatible SCPNs that feature an aliphatic polycarbonate backbone that undergoes dynamic covalent chemistry in conjunction with the dynamic aggregation of the “sticky” pendant units to “molecularly evolve” the SCPN’s primary structure. This strategy will allow SCPNs to “correct” non-optimal sequences by giving each polymer chain the ability to reshuffle its primary structure, ultimately allowing the SCPNs to achieve lower energy folding states. I predict that the hydrophobic cores of the resulting evolved SCPNs will be more enzyme-like and thus better suited for catalysis and targeted drug therapy applications.

Ámbito científico (EuroSciVoc)

• ciencias naturales › ciencias químicas › ciencia de polímeros
• ciencias naturales › ciencias biológicas › biología molecular › evolución molecular
• ciencias naturales › ciencias biológicas › bioquímica › biomoléculas › proteínas › enzima

Programa(s)

• H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions PROGRAMA PRINCIPAL
  
• H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
  

Tema(s)

• MSCA-IF-2015-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF)
  

Régimen de financiación

MSCA-IF-EF-ST - Standard EF

Convocatoria de propuestas

H2020-MSCA-IF-2015

Coordinador

TECHNISCHE UNIVERSITEIT EINDHOVEN

• Aportación neta de la UEn: € 165 598,80
• Dirección: GROENE LOPER 3 ; 5612 AE Eindhoven ; Países Bajos
• Región: Zuid-Nederland › Noord-Brabant › Zuidoost-Noord-Brabant
• Tipo de actividad: Higher or Secondary Education Establishments
• Coste total: € 165 598,80