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Model-Based Construction And Optimisation Of Versatile Chassis Yeast Strains For Production Of Valuable Lipid And Aromatic Compounds

Project description

Rational design of novel yeast strains producing lipid and aromatic compounds

Biotechnology harnesses biological processes for industrial and other purposes. Yeast are akin to tiny factories which are widely utilised for their metabolites, enzymes and proteins. The yeast Saccharomyces cerevisiae is perhaps the oldest and most used. The EU-funded CHASSY project plans to unlock the full potential of yeast by redesigning metabolic circuits and expanding the host range to include the oleaginous yeast Yarrowia lipolytica (able to accumulate significant amounts of lipids or triglycerides) and the thermotolerant yeast Kluyveromyces marxianus. The team will use synthetic biology tools to construct new strains for cell factories producing three high-value products: the oleochemicals docosanol and octanoic acid, and the aromatic molecule amorfrutin 1.

Objective

CHASSY will unlock the full potential of the yeasts Saccharomyces cerevisiae, Yarrowia lipolytica and Kluyveromyces marxianus as cell factories for production of high value compounds which have applications in the cosmetic, nutraceutical and white biotechnology sectors. Current cell factory strains for these classes of product are restricted to proof-of-principle levels because of limited precursor supply, poor product tolerance and lack of versatility. CHASSY addresses these challenges by redesigning metabolic circuits and expanding the host range to include the oleaginous yeast, Y. lipolytica and the thermotolerant yeast, K. marxianus. The systems biology approach will integrate model-based design, construction and analysis of yeast strains, resulting in reconfigured metabolic networks optimised for the production of lipid and aromatic molecules. Construction of the chassis strains, using new and existing synthetic biology tools, will be directed by knowledge derived from a thorough systems biology comparison of the three yeast species, conducted using integrative data analysis and genome scale metabolic models. The chassis strains will be used to build cell factories to produce three specific high value products: the oleochemicals, Docosanol and Octanoic acid; and the aromatic molecule, Amorfrutin 1. These new cell factory strains will be evaluated under industrial conditions to produce data that will further improve the chassis platforms. The major outcomes of this project will be (1) a new set of chassis yeast strains that are widely applicable for development of industrial cell factories; (2) the knowledge and technology to readily build and evaluate new chassis tailored to specific applications; (3) prototype cell factory strains producing three high value metabolites for commercial exploitation; (4) a dissemination and exploitation strategy to ensure that European SMEs benefit from the knowledge base, platform chassis and resources generated in CHASSY.

Call for proposal

H2020-NMBP-2016-2017

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Sub call

H2020-NMBP-BIO-2016

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Coordinator

UNIVERSITY COLLEGE CORK - NATIONAL UNIVERSITY OF IRELAND, CORK
Net EU contribution
€ 1 228 732,49
Address
Western road
T12 YN60 Cork
Ireland

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Region
Ireland Southern South-East
Activity type
Higher or Secondary Education Establishments
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Other funding
€ 0,00

Participants (9)