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Model-Based Construction And Optimisation Of Versatile Chassis Yeast Strains For Production Of Valuable Lipid And Aromatic Compounds

Objective

CHASSY will unlock the full potential of the yeasts Saccharomyces cerevisiae, Yarrowia lipolytica and Kluyveromyces marxianus as cell factories for production of high value compounds which have applications in the cosmetic, nutraceutical and white biotechnology sectors. Current cell factory strains for these classes of product are restricted to proof-of-principle levels because of limited precursor supply, poor product tolerance and lack of versatility. CHASSY addresses these challenges by redesigning metabolic circuits and expanding the host range to include the oleaginous yeast, Y. lipolytica and the thermotolerant yeast, K. marxianus. The systems biology approach will integrate model-based design, construction and analysis of yeast strains, resulting in reconfigured metabolic networks optimised for the production of lipid and aromatic molecules. Construction of the chassis strains, using new and existing synthetic biology tools, will be directed by knowledge derived from a thorough systems biology comparison of the three yeast species, conducted using integrative data analysis and genome scale metabolic models. The chassis strains will be used to build cell factories to produce three specific high value products: the oleochemicals, Docosanol and Octanoic acid; and the aromatic molecule, Amorfrutin 1. These new cell factory strains will be evaluated under industrial conditions to produce data that will further improve the chassis platforms. The major outcomes of this project will be (1) a new set of chassis yeast strains that are widely applicable for development of industrial cell factories; (2) the knowledge and technology to readily build and evaluate new chassis tailored to specific applications; (3) prototype cell factory strains producing three high value metabolites for commercial exploitation; (4) a dissemination and exploitation strategy to ensure that European SMEs benefit from the knowledge base, platform chassis and resources generated in CHASSY.

Call for proposal

H2020-NMBP-2016-2017

See other projects for this call

Sub call

H2020-NMBP-BIO-2016

Coordinator

UNIVERSITY COLLEGE CORK - NATIONAL UNIVERSITY OF IRELAND, CORK
Net EU contribution
€ 1 228 732,49
Address
Western Road
T12 YN60 Cork
Ireland
Activity type
Higher or Secondary Education Establishments
Non-EU contribution
€ 0,00

Participants (9)

TECHNISCHE UNIVERSITEIT DELFT
Netherlands
Net EU contribution
€ 975 091,25
Address
Stevinweg 1
2628 CN Delft
Activity type
Higher or Secondary Education Establishments
Non-EU contribution
€ 0,00
Region
West-Nederland Zuid-Holland Delft en Westland
CHALMERS TEKNISKA HOGSKOLA AB
Sweden
Net EU contribution
€ 652 135,00
Activity type
Higher or Secondary Education Establishments
Non-EU contribution
€ 0,00
Region
Södra Sverige Västsverige Västra Götalands län
INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT
France
Net EU contribution
€ 747 595,00
Address
147 Rue De L'universite
75007 Paris Cedex 07
Activity type
Research Organisations
Non-EU contribution
€ 0,00
JOHANN WOLFGANG GOETHE-UNIVERSITAET FRANKFURT AM MAIN
Germany
Net EU contribution
€ 535 500,00
Address
Theodor W Adorno Platz 1
60323 Frankfurt Am Main
Activity type
Higher or Secondary Education Establishments
Non-EU contribution
€ 0,00
Region
Baden-Württemberg Stuttgart Stuttgart, Stadtkreis
EVOLVA AG

Participation ended

Switzerland
Net EU contribution
€ 0,00
SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Address
Duggingerstrasse 23
4153 Reinach
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Non-EU contribution
€ 0,00
Region
Schweiz/Suisse/Svizzera Nordwestschweiz Basel-Landschaft
BIOPETROLIA AB
Sweden
Net EU contribution
€ 702 200,00
SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Address
Chalmers Tekniska Hogskola
412 96 Goteborg
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Non-EU contribution
€ 0,00
Region
Södra Sverige Västsverige Västra Götalands län
 
NOVA-INSTITUT FUR POLITISCHE UND OKOLOGISCHE INNOVATION GMBH
Germany
Net EU contribution
€ 251 000,00
SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Address
Chemiepark Knapsack Industriestrasse
50354 Huerth
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Non-EU contribution
€ 0,00
Region
Nordrhein-Westfalen Köln Rhein-Erft-Kreis
 
UCC ACADEMY DESIGNATED ACTIVITY COMPANY
Ireland
Net EU contribution
€ 212 500,00
Address
University College Cork National University Of Ireland Western Road
T12 Cork
Activity type
Other
Non-EU contribution
€ 0,00
Region
Ireland Eastern and Midland Dublin
 
CLARIANT PRODUKTE (DEUTSCHLAND) GMBH
Germany
Net EU contribution
€ 329 856,26
Address
Bruningstrasse 50
65929 Frankfurt Am Main
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Non-EU contribution
€ 0,00
Region
Darmstadt Frankfurt am Main, Kreisfreie Stadt