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Model-Based Construction And Optimisation Of Versatile Chassis Yeast Strains For Production Of Valuable Lipid And Aromatic Compounds

Description du projet

Conception rationnelle de nouvelles souches de levure produisant des composés lipidiques et aromatiques

La biotechnologie exploite notamment les processus biologiques à des fins industrielles. Les levures s’apparentent à de minuscules usines qui sont largement utilisées pour leurs métabolites, leurs enzymes et leurs protéines. Saccharomyces cerevisiae est peut-être la plus ancienne et la plus utilisée. Le projet CHASSY, financé par l’UE, prévoit de libérer tout le potentiel de la levure en repensant les circuits métaboliques et en élargissant la gamme d’hôtes pour ajouter la levure oléagineuse Yarrowia lipolytica (capable d’accumuler des quantités importantes de lipides ou de triglycérides) et la levure thermotolérante Kluyveromyces marxianus. L’équipe s’appuiera sur des outils de biologie synthétique pour créer de nouvelles souches destinées à des usines cellulaires fabriquant trois éléments de grande valeur: les produits oléochimiques docosanol et acide octanoïque, et la molécule aromatique amorfrutine 1.

Objectif

CHASSY will unlock the full potential of the yeasts Saccharomyces cerevisiae, Yarrowia lipolytica and Kluyveromyces marxianus as cell factories for production of high value compounds which have applications in the cosmetic, nutraceutical and white biotechnology sectors. Current cell factory strains for these classes of product are restricted to proof-of-principle levels because of limited precursor supply, poor product tolerance and lack of versatility. CHASSY addresses these challenges by redesigning metabolic circuits and expanding the host range to include the oleaginous yeast, Y. lipolytica and the thermotolerant yeast, K. marxianus. The systems biology approach will integrate model-based design, construction and analysis of yeast strains, resulting in reconfigured metabolic networks optimised for the production of lipid and aromatic molecules. Construction of the chassis strains, using new and existing synthetic biology tools, will be directed by knowledge derived from a thorough systems biology comparison of the three yeast species, conducted using integrative data analysis and genome scale metabolic models. The chassis strains will be used to build cell factories to produce three specific high value products: the oleochemicals, Docosanol and Octanoic acid; and the aromatic molecule, Amorfrutin 1. These new cell factory strains will be evaluated under industrial conditions to produce data that will further improve the chassis platforms. The major outcomes of this project will be (1) a new set of chassis yeast strains that are widely applicable for development of industrial cell factories; (2) the knowledge and technology to readily build and evaluate new chassis tailored to specific applications; (3) prototype cell factory strains producing three high value metabolites for commercial exploitation; (4) a dissemination and exploitation strategy to ensure that European SMEs benefit from the knowledge base, platform chassis and resources generated in CHASSY.

Appel à propositions

H2020-NMBP-2016-2017

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Sous appel

H2020-NMBP-BIO-2016

Coordinateur

UNIVERSITY COLLEGE CORK - NATIONAL UNIVERSITY OF IRELAND, CORK
Contribution nette de l'UE
€ 1 228 732,49
Adresse
WESTERN ROAD
T12 YN60 Cork
Irlande

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Région
Ireland Southern South-East
Type d’activité
Higher or Secondary Education Establishments
Liens
Coût total
€ 1 228 732,49

Participants (9)