Cel
CHASSY will unlock the full potential of the yeasts Saccharomyces cerevisiae, Yarrowia lipolytica and Kluyveromyces marxianus as cell factories for production of high value compounds which have applications in the cosmetic, nutraceutical and white biotechnology sectors. Current cell factory strains for these classes of product are restricted to proof-of-principle levels because of limited precursor supply, poor product tolerance and lack of versatility. CHASSY addresses these challenges by redesigning metabolic circuits and expanding the host range to include the oleaginous yeast, Y. lipolytica and the thermotolerant yeast, K. marxianus. The systems biology approach will integrate model-based design, construction and analysis of yeast strains, resulting in reconfigured metabolic networks optimised for the production of lipid and aromatic molecules. Construction of the chassis strains, using new and existing synthetic biology tools, will be directed by knowledge derived from a thorough systems biology comparison of the three yeast species, conducted using integrative data analysis and genome scale metabolic models. The chassis strains will be used to build cell factories to produce three specific high value products: the oleochemicals, Docosanol and Octanoic acid; and the aromatic molecule, Amorfrutin 1. These new cell factory strains will be evaluated under industrial conditions to produce data that will further improve the chassis platforms. The major outcomes of this project will be (1) a new set of chassis yeast strains that are widely applicable for development of industrial cell factories; (2) the knowledge and technology to readily build and evaluate new chassis tailored to specific applications; (3) prototype cell factory strains producing three high value metabolites for commercial exploitation; (4) a dissemination and exploitation strategy to ensure that European SMEs benefit from the knowledge base, platform chassis and resources generated in CHASSY.
Dziedzina nauki
- nauki przyrodniczenauki biologicznegenetyka i dziedzicznośćgenom
- nauki przyrodniczenauki chemicznechemia organicznazwiązki aromatyczne
- nauki przyrodniczeinformatykanauka o danychanaliza danych
- nauki przyrodniczenauki biologicznebiologia ewolucyjna
- nauki przyrodniczenauki chemicznechemia organicznaaminy
- nauki przyrodniczenauki biologicznebiochemiabiocząsteczkibiałkaproteomika
- nauki przyrodniczenauki biologicznebiologia syntetyczna
- inżynieria i technologiabiotechnologia przemysłowainżynieria metaboliczna
- nauki przyrodniczenauki biologicznebiochemiabiocząsteczkilipidy
Program(-y)
Zaproszenie do składania wniosków
H2020-NMBP-BIO-2016
Zobacz inne projekty w ramach tego zaproszenia
System finansowania
RIA - Research and Innovation action
Koordynator
T12 YN60 Cork
Irlandia
Uczestnicy (9)
2628 CN Delft
41296 Goeteborg
75007 Paris Cedex 07
60323 Frankfurt Am Main
Zakończenie uczestnictwa
4153 Reinach
412 96 Goteborg
50354 Huerth
T12 Cork
65929 Frankfurt Am Main
